We narrowed to 13,583 results for: TIM

1. pfTRAP(VWA)

   Plasmid

   #50049

   Purpose

   Expresses plasmodium falciparum TRAP VWA domain (41-240) in mammalian cells

   Depositor

   Timothy Springer

   Article

   Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

   Insert

   pfTRAP(vwa) (TRAP Synthetic)
   

   Use

   Tags

   6Xhis tag

   Expression

   Mammalian

   Mutation

   C55G, N132S

   Promoter

   CMV

   Available since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

2. MSP2266

   Plasmid

   #70705

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMC-6-RLuc-9

   Plasmid

   #197739

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   Renilla luciferase (tomato optimized)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBS-L1PA4CHmneo

   Plasmid

   #69607

   Purpose

   Expresses a neomycin tagged codon optimized L1PA4

   Depositor

   Astrid Roy-Engel

   Article

   Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

   Insert

   L1PA4
   

   Use

   Tags

   mneo retrotransposition cassette

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Ilmol

   Plasmid

   #120310

   Purpose

   A red-shifted GEVI consists of triple mutant ciona based voltage sensing domain with optimized linker and dTomato K159R.

   Depositor

   Bradley Baker

   Article

   Yi et al Sci Rep. 2018 Oct 12;8(1):15199. doi: 10

   Insert

   Ilmol
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCXLE-dCas9GFP-shP53

   Plasmid

   #102906

   Purpose

   EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   dCas9GFP-shP53
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pXWJ109As-gfp

   Plasmid

   #123143

   Purpose

   An arsenic sensor with optimized intracellular receptor density. pSB3K3 carrying J109-30arsR-t-ParsR-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-30arsR-t-ParsR-30gfp-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J109, ParsR

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSR47

   Plasmid

   #69258

   Purpose

   pmyo-3::CRE for integration on ttTi14024, Chr X

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Insert

   Cre recombinase
   

   Use

   Cre/Lox; Mossci

   Tags

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0

   Promoter

   myo-3

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BAT-lux

   Plasmid

   #20890

   Depositor

   Stefano Piccolo

   Article

   Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

   Insert

   7xTCF binding sites, Siamois minimal promoter
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   7 multimerized TCF-binding elements fused upstrea…

   Promoter

   Available since

   April 22, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pET28a-HsSENP7

    Plasmid

    #71470

    Purpose

    Encodes human full length SENP7. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 7 (SENP7 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pCSN074

    Plasmid

    #166731

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-NLS
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCEP4-25711gp160

    Plasmid

    #111841

    Purpose

    Mammalian expression plasmid for Env from the 25711 HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (25711.2) Env
    

    Use

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCEP4-AD8gp160

    Plasmid

    #123260

    Purpose

    Mammalian expression plasmid for Env from the AD8 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (AD8) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCEP4-CH115gp160

    Plasmid

    #123280

    Purpose

    Mammalian expression plasmid for Env from the CH115 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (CH115) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pALPS_3'LTR GFP@gag start SFFV rev

    Plasmid

    #101339

    Purpose

    LTR drives GFP and internal SFFV promoter drives codon optimized rev

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    GFP, rev
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEG BacMam N term myc eGFP 3C

    Plasmid

    #160682

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Use

    Tags

    myc eGFP 3C

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUPD2 SV40-AcrIIA4 (GB3333)

    Plasmid

    #160605

    Purpose

    SV40-AcrIIA4 codon optimized for N.benthamiana

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

    Insert

    AcrIIA4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-parkin W403A

    Plasmid

    #45974

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin W403A (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    tac

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEBB-HA-hILP del RING

    Plasmid

    #25675

    Depositor

    Colin Duckett

    Article

    Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Encodes aa 1-449 only. aa 450-497 have been dele…

    Promoter

    Available since

    Aug. 11, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pCDNA3_AKAR5Δ

    Plasmid

    #119912

    Purpose

    Mammalian expression of the AKAR5Δ sensor with N-terminal His tag for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    AKAR5Δ
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pZyxin-mMaple2

    Plasmid

    #101150

    Purpose

    Fluorescent protein for superresolution imaging and a focal adhesion protein for binding substrate

    Depositor

    Xiaowei Zhuang

    Article

    Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

    Insert

    human codon optimized mMaple2 fused to zyxin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEX-parkin C431S

    Plasmid

    #45976

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin C431S (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    tac

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pNC1137

    Plasmid

    #41563

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    Ura3-FUS1-UBIMdkGFP*-SpHis5-Tim9
    

    Use

    Tags

    Expression

    Mutation

    GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGK415-­phlTA2-­1E

    Plasmid

    #165970

    Purpose

    Expresses phlTA(2-­1E) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    phlF
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal and three copies of V…

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

    Promoter

    pPGK1 mutant

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRAN334

    Plasmid

    #120814

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

    Insert

    cyan fluorescent protein, codon-optimized for low GC organisms
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYPQ292-D570A

    Plasmid

    #129679

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b
    

    Use

    CRISPR; Gateway compatible aacas12b entry clone

    Tags

    Expression

    Plant

    Mutation

    D570A; AaCas12b is rice codon optimized

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRK5_tAKARγ

    Plasmid

    #119915

    Purpose

    Mammalian expression of the tAKARγ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARγ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTL472

    Plasmid

    #69882

    Purpose

    dCirl promoter region was replaced with an optimized 20xUAS-IVS promoter cassette

    Depositor

    Robert Kittel, Tobias Langenhan

    Article

    Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

    Insert

    CIRL (Cirl Fly)
    

    Use

    Transformation vector

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKT-mCitrineV163A-HIS

    Plasmid

    #64685

    Purpose

    pKT vector encoding yeast-codon optimized yellow fluorescent protein mCitrine V163A. The V163A mutation confers fast protein maturation.

    Depositor

    Erin O'Shea

    Article

    Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

    Insert

    mCitrine V163A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Valine 163 changed to alanine

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMM532

    Plasmid

    #112529

    Purpose

    pZA1D-hrtR - HrtR expressed consitutively from promoter ProD with RBS2, p15a origin, AmpR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Insert

    HrtR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ProD

    Available since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCEP4-SERTG56A -myc

    Plasmid

    #107459

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G56A conferring wildtype phenotype

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G56A

    Promoter

    CMV

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pALPS_3'LTR GFP@gag start SFFV tat

    Plasmid

    #101338

    Purpose

    LTR drives GFP and internal SFFV promoter drives codon optimized tat

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    GFP, tat
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro

    Plasmid

    #89994

    Purpose

    Donor template for generation of NGN3-ntdTomato reporter cell lines

    Depositor

    Timo Otonkoski

    Insert

    NGN3-T2A-NLS-TdTomato-ires-puro-BGHpA-PGK-puro (NEUROG3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCEP4-001428gp160

    Plasmid

    #123255

    Purpose

    Mammalian expression plasmid for Env from the 001428 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (001428) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCSN069

    Plasmid

    #166728

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-Mxi1
    

    Use

    CRISPR

    Tags

    Mxi1

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSR35

    Plasmid

    #69256

    Purpose

    phsp16.41::CRE for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Mossci

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    hsp16.41

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJAID5VY

    Plasmid

    #165048

    Purpose

    Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    OsTIR1 is codon-optimised for expression in S. ce…

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGEX-parkin F463A

    Plasmid

    #45975

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin F463A (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    tac

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET29NK-LGK/mGFPmut3

    Plasmid

    #81168

    Purpose

    GFP fusion to levoglucosan kinase

    Depositor

    Timothy Whitehead

    Article

    Wrenbeck et al ACS Synth Biol. 2019 Mar 15;8(3):474-481

    Insert

    Levoglucosan kinase wild-type
    

    Use

    Tags

    His6x and mGFPmut3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSR28

    Plasmid

    #69253

    Purpose

    phlh-8::CRE for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Mossci

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    hlh-8

    Available since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pK3FS-PYK1

    Plasmid

    #85777

    Purpose

    N-ICE plasmid pK3FS-PYK1

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-KanMX-loxP-PYK1p-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pYPQ291

    Plasmid

    #129671

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    BthCas12b
    

    Use

    CRISPR; Gateway compatible bthcas12b entry clone

    Tags

    Expression

    Plant

    Mutation

    BthCas12b is rice codon optimized

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAAV-EF1a-DIO-C1V1(t/t)-mRuby2-KV2.1-WPRE-SV40

    Plasmid

    #124604

    Purpose

    Cre-dependent soma-localized C1V1(t/t) for optogenetic stimulation

    Depositor

    Christopher Harvey

    Article

    Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

    Insert

    Soma-localized C1V1(t/t)
    

    Use

    AAV

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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44. JH05

    Plasmid

    #73516

    Purpose

    mYPet_PH pCFJ150 - contain C. elegans codon optimized mYPet

    Depositor

    Bob Goldstein

    Article

    Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

    Insert

    pmex-5::mPYet::PH::tbb-2
    

    Use

    Tags

    mYPet

    Expression

    Mutation

    Promoter

    mex-5

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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45. pcDNA5/FRT/TO-Flag-Tipin

    Plasmid

    #23117

    Depositor

    Aziz Sancar

    Article

    Kemp et al J Biol Chem. 2010 Mar 15. ():.

    Insert

    Tipin (TIPIN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

46. pYPQ290-D570A

    Plasmid

    #129673

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AacCas12b
    

    Use

    CRISPR; Gateway compatible aaccas12b-d570a entry …

    Tags

    Expression

    Plant

    Mutation

    D570A; AacCas12b is rice codon optimized

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCEP4-H031gp160

    Plasmid

    #123272

    Purpose

    Mammalian expression plasmid for Env from the H031 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (H031) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. pEG584

    Plasmid

    #40191

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (RNAi resistant) (mex-5 Nematode)
    

    Use

    Tags

    Dendra2 PAFP (sequence optimized for worm express…

    Expression

    Worm

    Mutation

    Exon 2 recoded to be RNAi-resistant

    Promoter

    4.4 kb mex-5 promoter fragment

    Available since

    Sept. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

49. pSB4C5_J23101-CocE

    Plasmid

    #128129

    Purpose

    Expressing CocE enzyme (cocaine to benzoate) gene under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    CocE
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. pRK5_tAKARζ

    Plasmid

    #119918

    Purpose

    Mammalian expression of the tAKARζ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARζ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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