We narrowed to 26,940 results for: sta

1. RpH-ILV-FRB

   Plasmid

   #225137

   Purpose

   RpH-ILV probe strongly colocalises with lysosomal markers with FRB

   Depositor

   Massimiliano Stagi

   Article

   Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONOR223 R2pH-LAMP1-3xFLAG

   Plasmid

   #157941

   Purpose

   gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

   Depositor

   Massimiliano Stagi

   Article

   Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

   Insert

   R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
   

   Use

   Gateway system

   Tags

   3XFLAG, mCherry, and pHluorin

   Expression

   Mutation

   Promoter

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. DN2-Gli3 in AINGpCAGGS

   Plasmid

   #121969

   Purpose

   strong Gli3 transcriptional activator

   Depositor

   James Briscoe

   Article

   Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

   Insert

   DN2Gli3 (GLI3 Human)
   

   Use

   Chick electroporation

   Tags

   5xmyc

   Expression

   Mammalian

   Mutation

   N terminus deletion (nt1-1401)

   Promoter

   Available since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV14_NHP2L1(15.5kD)

   Plasmid

   #73065

   Purpose

   expression clone for human NHP2L1 (15.5kD) with C-terminal 3xFLAG-tag

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   NHP2L1 (15.5kD) (SNU13 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1_hE2F7mg

   Plasmid

   #73073

   Purpose

   human E2F7 minigene (exons 11,12,13/introns cassette)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   E2F7 (E2F7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partial gene, exons 11,12,13 spaced by introns

   Promoter

   CMV

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ePB-PURO-FLAG-METTL3-APPA

   Plasmid

   #160253

   Purpose

   For stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposase

   Depositor

   Alessandro Fatica

   Article

   Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changes in aa 395–398, DPPW → APPA

   Promoter

   tight TRE promoter

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPBbsr2-Necl-5-ScNeo

   Plasmid

   #170283

   Purpose

   To monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

   Insert

   a recombinant mouse Necl-5 fused to the mScarlet and to the mNeonGreen fluorescent protein (Pvr Synthetic, Mouse)
   

   Use

   Tags

   mScarlet and mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pD2sE_SC10

   Plasmid

   #219716

   Purpose

   DV2 stable E dimer with fusion loop mutation

   Depositor

   Brian Kuhlman

   Article

   Kudlacek et al Sci Adv. 2021 Oct 15;7(42):eabg4084. doi

   Insert

   DV2 sE SC10
   

   Use

   Tags

   8xHis and human serum albumin (HSA)

   Expression

   Mammalian

   Mutation

   G106D, A259W, T262R, F279W, T280P

   Promoter

   Available since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3-B-161;+80pCalb2

   Plasmid

   #66745

   Purpose

   luciferase reporter for Calb2 promoter (-161to +80, WT)

   Depositor

   Emanuela Felley-Bosco, Rolf Stahel

   Article

   Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

   Insert

   Calb2 promoter (-161bp+80) (CALB2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Calb2

   Available since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSAVED-CHAT_PCaspase_PCi_PC-σ

    Plasmid

    #214040

    Purpose

    Bacterial expression plasmid for SAVED-CHAT, PCaspase, PCi, and PC-σ from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-Pcaspase-Pci-PC-σ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lacUV5 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGL3-B-264;+80pCalb2

    Plasmid

    #66746

    Purpose

    luciferase reporter for Calb2 promoter (-264 to +80, WT)

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-264bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Calb2

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL3-B-419;+80pCalb2

    Plasmid

    #66747

    Purpose

    luciferase reporter for Calb2 promoter (-419 to +80, WT)

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-419bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Calb2

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-ALK2 K235R

    Plasmid

    #80872

    Purpose

    mammalian expression of ALK2 K235R

    Depositor

    Aristidis Moustakas

    Article

    Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

    Insert

    ALK2 (ACVR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K235R

    Promoter

    CMV

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

14. DN1-Gli3 in AINGpCAGGS

    Plasmid

    #121970

    Purpose

    medium Gli3 transcriptional activator

    Depositor

    James Briscoe

    Article

    Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

    Insert

    DN2Gli3 (GLI3 Human)
    

    Use

    Chick electroporation

    Tags

    5xmyc

    Expression

    Mammalian

    Mutation

    N terminus deletion (nt1-864)

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 3019_pETcon-SARS-CoV-2-RBD_E484K

    Plasmid

    #184404

    Purpose

    yeast surface display of the SARS-CoV-2 Eta variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    E484K

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1_ZKSCAN1_Tau7-12WT

    Plasmid

    #194166

    Purpose

    Insert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 7,9-12. Expresses the tau circRNA 12-->7 WT with 3X flag tag in exon 7.

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    Microtubule-associated protein tau 7-12 WT
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1_ZKSCAN1_Tau10-12WT

    Plasmid

    #194163

    Purpose

    Insert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 10-12. Expresses the tau circRNA 12-->10 WT with 3X flag tag in exon 10.

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    Microtubule-associated protein tau 10-12 WT ZKSCAN1 intronic alu elements
    

    Use

    Tags

    3X flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTER GAMMA-TUBULIN shRNA Human

    Plasmid

    #87955

    Purpose

    reduced the expression of gamma-tubulin in human cells

    Depositor

    Ana Carrera, Maria Alvarado-Kristensson

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    sh gamma-tubulin (TUBG1 Human)
    

    Use

    Tags

    No-tag

    Expression

    Mammalian

    Mutation

    The protein is a sh-gamma-tubulin resistant gene.…

    Promoter

    Available since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLAP-BUBR1-dKARD

    Plasmid

    #114053

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    BUBR1-dKARD (BUB1B Human)
    

    Use

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    C2823A and G2826A (siRNA-resistance): AGATACTAGCT…

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSN-A112C-GpA-G83I

    Plasmid

    #191239

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    

    Use

    Tags

    nuclease A from S. aureus fused to the TM domain …

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and Glycin…

    Promoter

    T7 promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge