We narrowed to 20,245 results for: ACE;

1. pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC

   Plasmid

   #22931

   Purpose

   Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PDZ binding motif (delta C)

   Depositor

   Alexandra Newton

   Article

   Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

   Insert

   PHLPP1-deltaC (PHLPP1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PHLPP-deltaC, deletion of PDZ binding domain--the…

   Promoter

   CMV

   Available Since

   Feb. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

2. MTK7_014

   Plasmid

   #123881

   Purpose

   Encodes the extended 3rd gen. 3' HIV LTR cassette as a type 7 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   3' HIV LTR (3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MTK3b_031

   Plasmid

   #123809

   Purpose

   Encodes Kruppel Associated Box repression domain fused to methyl CpG binding protein 2 as a Type 3b part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   KRAB:meCP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. GW1-mito-mRuby2-roGFP1

   Plasmid

   #107219

   Purpose

   Mammalian expression plasmid to target the rmRuby1-roGFP1 sensor to the mitochondrial matrix.

   Depositor

   Mathew Tantama

   Article

   Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

   Insert

   mRuby2-roGFP1
   

   Use

   Tags

   4xCox8

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOBCol3.6-GFPtpz

   Plasmid

   #110211

   Purpose

   Labels immature osteoblasts and connective tissue cells expressing type 1 collagen.

   Depositor

   David Rowe

   Article

   Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

   Insert

   eGFPtopaz
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   None

   Promoter

   3.6 kb rat type 1 collagen + first intron

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBTK615

   Plasmid

   #110609

   Purpose

   BTK assembled plasmid - used in Stage 2 assembly to target Cas9 or dCas9

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   sgRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TetO-FUW-Hes5

   Plasmid

   #61536

   Purpose

   Expresses Hes5 in mammalian cells

   Depositor

   Rudolf Jaenisch

   Article

   Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

   Insert

   Hes5 (Hes5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST

   Plasmid

   #127315

   Purpose

   Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and CL1/PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCXLE-hS + eGFP

   Plasmid

   #74945

   Purpose

   Dosage control and tracing of reprogramming episomes

   Depositor

   Ann Zovein

   Article

   Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

   Insert

   Sox2 (SOX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

10. mADAM17deltaMP in pQCXIP cFLAG

    Plasmid

    #19144

    Depositor

    Zena Werb

    Article

    Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

    Insert

    ADAM17 (Adam17 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    delta 189-378

    Promoter

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST

    Plasmid

    #127312

    Purpose

    Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAV105

    Plasmid

    #63195

    Purpose

    pRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAV104

    Plasmid

    #63194

    Purpose

    pRS404 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Sept. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ROZA-XL

    Plasmid

    #64194

    Purpose

    Fluorescent reporter for ZAP-70 tyrosine kinase activity

    Depositor

    Annemarie Lellouch

    Article

    Cadra et al Biochem Biophys Res Commun. 2015 Feb 28.

    Insert

    ROZA-XL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ROZA-XL

    Promoter

    CMV

    Available Since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

15. MTK234_032

    Plasmid

    #123995

    Purpose

    Encodes the spCas9 sgRNA1 hAAVS1 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA1 -hAAVS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAV123

    Plasmid

    #63189

    Purpose

    pRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CBA-DIO-GCaMP6s-P2A-mBeRFP-WPRE-pA

    Plasmid

    #175177

    Purpose

    Cre-dependent GCaMP6s and mBeRFP expression

    Depositor

    Bernardo Sabatini

    Article

    Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    P2A-mBeRFP

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAH18

    Plasmid

    #154397

    Purpose

    Derived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Fused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    NA

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAV106

    Plasmid

    #63196

    Purpose

    pRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds

    Plasmid

    #119074

    Purpose

    Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    ubb:MCP-p65-HSF1-2a-Cerulean
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish ubiquitin promoter

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds

    Plasmid

    #119075

    Purpose

    Zebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    ubb:Com-KRAB-2a-Cerulean
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish ubiquitin promoter

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pVC-Ds-DrU6a:sgRNA-com-Ds

    Plasmid

    #119077

    Purpose

    To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:2xCom-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pVC-Ds-DrU6a:sgRNA-MS2-Ds

    Plasmid

    #119076

    Purpose

    To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:2xMS2-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLEX_970_puro_DEST_V5_BFP

    Plasmid

    #45297

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    BFP
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

25. AAVS1-Puro Xlone SOX17

    Plasmid

    #179838

    Purpose

    Doxycycline-inducible expression of human SOX17

    Depositor

    Xiaoping Bao

    Article

    Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

    Insert

    SOX17 (SOX17 Human)
    

    Use

    AAV, CRISPR, Synthetic Biology, and TALEN ; Donor…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAV124

    Plasmid

    #63190

    Purpose

    pRS424 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pT2ADW-lox-mCherryNLS-pA-lox-4493NES

    Plasmid

    #138374

    Purpose

    Booster-PKA for the generation of mice

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    lox-mCherry-lox-Booster-PKA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MTK234_033

    Plasmid

    #123996

    Purpose

    Encodes the spCas9 sgRNA1 hAAVS1 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA2 -hAAVS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MTK234_034

    Plasmid

    #123997

    Purpose

    Encodes the spCas9 sgRNA1 hAAVS1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA3 -hAAVS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MTK0_031

    Plasmid

    #123970

    Purpose

    Encodes the BxBI-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    BxBI-hygro-mRuby2PEST landing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge