We narrowed to 8,809 results for: CAG

1. L4440_BioBrick-hif-1-sgRNA-B

   Plasmid

   #177785

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   hif-1 (hif-1 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKDsgRNA-rssA

   Plasmid

   #89957

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   rssA gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXAT2

   Plasmid

   #80494

   Purpose

   AAVS1 sgRNA expression vector

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   AAVS1 sgRNA-T2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP shRNA tet pLKO puro

   Plasmid

   #163017

   Purpose

   Control tet-inducible shRNA targeting eGFP

   Depositor

   Mark Rubin

   Article

   Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVP91A-pcaHG

   Plasmid

   #113766

   Purpose

   Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.

   Depositor

   John Lipscomb

   Article

   Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11

   Inserts

   β subunit of Protocatechuate 3,4- Dioxygenase
   α subunit of Protocatechuate 3,4- Dioxygenase
   

   Use

   Tags

   Met-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T5 promoter

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPPC019.AAV

   Plasmid

   #171147

   Purpose

   Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   hAAVS1 scRNA
   J1-BBa_J23117-mRFP
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119 and J1-BBa_J23117

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIS1-Eef25UTR-TOPmut-renilla

   Plasmid

   #38236

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   Eef2_TOPm_5UTR/Renilla (Eef2 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   The first nucleotides of the 5' UTR were cha…

   Promoter

   Endogenous Eef2

   Available since

   Nov. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pU6-Sp-pegRNA-HEK3_CTT_ins

   Plasmid

   #132778

   Purpose

   S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   HEK3_CTT_ins pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   U6

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDECKO_GFP

   Plasmid

   #72619

   Purpose

   Expresses two gRNAs targeting GFP

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

   Insert

   gRNAs toward GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (gRNA1) and H1 (gRNA2)

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459-HypaCas9-mR2-AAVS1_sgRNA

    Plasmid

    #183890

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. T/brachyury(2A-Dre)

    Plasmid

    #140761

    Purpose

    Targeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.

    Depositor

    Hideyuki Okano

    Article

    Serizawa et al Development. 2019 Nov 4;146(21). pii: de

    Insert

    Dre
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Endogenous T/brachyury

    Available since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHL-avitag3

    Plasmid

    #99847

    Purpose

    mammalian expression with secretive signal and avi tag for biotinylation

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EPHB2 Human)
    

    Use

    Tags

    Avi

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. LLP791_PB_dCas9-SSSavi_BirA

    Plasmid

    #211786

    Purpose

    PiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    dCas9, Spy, Snoop, aGCN4, AviTags
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    dCas9 D10A and H840A

    Promoter

    pCAGG abd pEF1a

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAPM-D4 miR30-TRIM28 ts1

    Plasmid

    #115862

    Purpose

    TRIM28 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    TRIM28 shRNA (TRIM28 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE

    Plasmid

    #176273

    Purpose

    Expression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI sgRNA (sgTet) and BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPRv2-sgAAVS1-HepmTurquoise2

    Plasmid

    #192828

    Purpose

    Expresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mTurquoise2 from hepatocyte-specific promoter

    Depositor

    Kristin Knouse

    Article

    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

    Insert

    mTurquoise2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for sgRNA and hepatocyte-specific promoter (HS…

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pv6_CBX1_2xChromo

    Plasmid

    #179393

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX1_2xChromo
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. dCas9-10xSunTag(22aa)

    Plasmid

    #215745

    Purpose

    Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    dCas9-10xSunTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta-actin promoter

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHL-FcHis

    Plasmid

    #99846

    Purpose

    mammalian expression with secretive signal and FC tag

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EPHB2 Human)
    

    Use

    Tags

    Fc_His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TDP43 NOTAG1

    Plasmid

    #28206

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Full length

    Promoter

    Available since

    July 1, 2011

    Availability

    Academic Institutions and Nonprofits only

21. pAV116

    Plasmid

    #63183

    Purpose

    pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAV126

    Plasmid

    #63192

    Purpose

    pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AICSDP-63: ACTN2-mEGFP

    Plasmid

    #124607

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Insert

    ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. D. vulgaris sp2 CRISPR/pACYCDuet-1

    Plasmid

    #81186

    Purpose

    Expresses CRISPR array containing 3 copies of Desulfovibrio vulgaris spacer 2 and flanking repeats in E. coli

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Synthetic CRISPR array (3 copies of D. vulgaris spacer #2 flanked by repeats)
    

    Use

    CRISPR

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pSEM376 - sgRNA - spc4 - array - insertion - unc-119 - locus

    Plasmid

    #182344

    Purpose

    sgRNA4 for insertion of extrachromosomal arrays into the ce-unc-119 genomic locus. Use with pSEM371 fragment included in Ex array.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Spacer 4
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGCP123-EbpA_g1

    Plasmid

    #153517

    Purpose

    sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

    Depositor

    Kimberly Kline

    Article

    Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

    Insert

    pnisA-sgRNA(EbpA_g1)-dCas9 scaffold
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MBP-TSF-TEVcs-ULK1

    Plasmid

    #171416

    Purpose

    For the purifcation of human ULK1 complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ULK1 (ULK1 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. L4440_BioBrick-SCR-sgRNA-B

    Plasmid

    #177812

    Purpose

    Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    Scrambled sgRNA targeting sequences
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PX459-sgRNA048_Grb10

    Plasmid

    #232934

    Purpose

    Expression vector for a sgRNA against the mouse Grb10 locus and SpCas9 with T2A-Puromycin resistance gene.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    Cas9-T2A-PuroR (Grb10 S. pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pXPR_502_sgCD45

    Plasmid

    #158705

    Purpose

    CD45 CRISPRa control

    Depositor

    David Root, John Doench

    Article

    Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

    Insert

    CD45 (PTPRC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge