We narrowed to 9,400 results for: Coli
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Plasmid#78671PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [E:36INS1-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS1-pBAD_AB
Plasmid#78670PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_I13453 to adjust spacing in MoClo system. [A:36INS1-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK136
Plasmid#71122PurposeExpression of Acetobacter aceti 1023 citrate synthase (AarA) in E. coliDepositorInsertcitrate synthase
UseTagsExpressionBacterialMutationPromoterT7Available SinceJan. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFLIP34
Plasmid#65501PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7TFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP35
Plasmid#65502PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP36
Plasmid#65504PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-Cer-cMycExpressionMutationPromoterT7 promoterAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP42
Plasmid#65617PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-Cit-attR1 and attR2-mCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-A
Plasmid#64979PurposeExpresses the E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E108APromoterAvailable SinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-K
Plasmid#64978PurposeExpresses the E105K mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E105KPromoterAvailable SinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Cow 1-82)
Plasmid#62062PurposeExpression of Cow p53 (residues 1-82) in e. coliDepositorInsertp53
UseTagsHisExpressionBacterialMutationcontains TAD residues 1-82PromoterT7Available SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Rabbit 1-87)
Plasmid#62058PurposeExpression of Rabbit p53 (residues 1-87) in e. coliDepositorInsertp53
UseTagsHisExpressionBacterialMutationcontains TAD residues 1-87PromoterT7Available SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1506
Plasmid#53026PurposePLlacO-1:RBS(st7):csrADepositorInsertcsrA
UseSynthetic BiologyTagsRBS(st7)ExpressionBacterialMutationPromoterpLlac0-1Available SinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-CylI
Plasmid#48200PurposeExpresses CylI (type III PKS) in E. coliDepositorInsertCylI
UseTags6x His tagExpressionBacterialMutationPromoterT7Available SinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-29b-CylB
Plasmid#48199PurposeExpresses CylB (acyl carrier protein) in E. coliDepositorInsertCylB
UseTags6x His tagExpressionBacterialMutationPromoterT7Available SinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
p70rg 11
Plasmid#17830DepositorInsertE. coli optimized red flourescent protein (RFPec), GFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET His10-Draper delta ITAM
Plasmid#126671PurposeExpression of His10-Draper delta ITAM mutant for purification from E. coliDepositorInsertDraper cytoplasmic ITAM residues mutated to Phe
UseTagsExpressionBacterialMutationCytoplasmic ITAM residues mutated to PhePromoterAvailable SinceAvailabilityAcademic Institutions and Nonprofits only -
pAKW.114A-PKA
Plasmid#154051PurposeExpresses PKA in E. coliDepositorInsertProtein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)
UseTagsExpressionBacterialMutationPromoterpCinAvailable SinceAvailabilityAcademic Institutions and Nonprofits only -
piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)
Plasmid#209086PurposeNgn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expressionDepositorUseTagsAviTagExpressionMammalianMutationhuman codon optimizedPromoterTRE4G and hSynAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)
Plasmid#209085PurposeNgn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expressionDepositorUseTagsExpressionMammalianMutationhuman codon optimizedPromoterTRE4G and hSynAvailable SinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDB327
Plasmid#113671Purposeexpresses Ulp1_R3 in Escherichia coliDepositorInsertUlp1_R3
UseTags6-HisExpressionBacterialMutationcontains solubility enhancing mutationsPromoterT7Available SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRE4-Bpa
Plasmid#155342PurposePlasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.DepositorInserthumanized EBpaRS/BstYam
UseTagsExpressionMammalianMutationPromoterPGK/U6Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet1-tglB
Plasmid#134676PurposeExpresses His6-TglBin E. ColiDepositorInsertTglB
UseTagsHis6ExpressionBacterialMutationPromoterT7Available SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-EK_C122S_His5
Plasmid#49048PurposeExpresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coliDepositorInsertEnteropeptidase, peptidase domain (TMPRSS15 Bovine)
UseTags5xHisExpressionBacterialMutationP92R, E185D, C122SPromoterT7Available SinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac2.0
Plasmid#119872PurposeCumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPveg promoter with CuO sequence and PxylR promoterAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac1.8
Plasmid#119871PurposeConstitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR'
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationSNP in 592 bpPromoterPserA promoter and Pveg promoter with CuO sequenceAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
His SUMO HNRNPA1 dHexa
Plasmid#197013PurposeE. coli expression vectorDepositorInsertHNRNPA1
UseTags6His-SUMOExpressionBacterialMutationPromoterAvailable SinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC-LYCipi
Plasmid#53279PurposeContains crtE, crtB, crtI, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces lycopene in Escherichia coliDepositorInsertidi
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
BPK2014-AaCas12b
Plasmid#121949PurposeE. coli expression, protein purificationDepositorInsertAaCas12b
UseTags10xHis tagExpressionBacterialMutationPromoterAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCN34e-ktrA-yfp
Plasmid#227640Purposec-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.DepositorInsertktrA-yfp
UseTagsExpressionBacterialMutationPromoterAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI3M-TBD
Plasmid#113077PurposeExpresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI3M-TBD
UseTagsExpressionBacterialMutationPromoterAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only