We narrowed to 11,805 results for: NSI;
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Plasmid#159364PurposeMammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at C-terminus.DepositorInsertSARS-CoV-2 S protein (S SARS-CoV-2)
TagsFlagExpressionMammalianMutationFurin-cleavage site mutationPromoterCMVAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBacI-KDAC8 H143A
Plasmid#224344PurposeExpresses human KDAC8 (HDAC8) H143A in insect cellsDepositorInsertKDAC8 (HDAC8 Human)
TagsTEV-cleavable His6ExpressionInsectMutationHistidine 143 mutated to alaninePromoterPolyhedrinAvailable SinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA8-Flag-LARS(1-976aa)
Plasmid#139689PurposeExpresses N-teminal Flag tagged aa 1-976 of LARS1DepositorAvailable SinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
anti-VGluT1 scFv [N28/9] with sortase tag
Plasmid#204424PurposeMammalian Expression of anti-VGluT1 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N28/9.DepositorInsertRecombinant mouse scFv targeting VGluT1 (Rattus norvegicus)
Tags6xHis tag and Sortase tagExpressionMammalianAvailable SinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
anti-PSD-95 scFv [K28/43] with sortase tag
Plasmid#204422PurposeMammalian Expression of anti-PSD-95 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma K28/43.DepositorInsertRecombinant mouse scFv targeting PSD-95 (Homo sapiens)
Tags6xHis tag and Sortase tagExpressionMammalianAvailable SinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-oScarlet
Plasmid#137138PurposeIntersectional viral expression of oScarlet in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationE95DPromoterEF1aAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Myc-PGK-Puro-IRES-GFP
Plasmid#75124PurposeRetroviral expression plasmid encoding wild type murine MycDepositorAvailable SinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-D594N
Plasmid#131713PurposeMammalian Expression of Flag tagged BRAFDepositorAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_CRFmut
Plasmid#208663PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in a cre-dependent mannerDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
UseAAVPromoterEFSAvailable SinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
VBS mutant of alpha catenin sensor
Plasmid#71710PurposeThis form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.DepositorInsertalpha-catenin ΔVBS FRET Sensor
TagsECFP and YPETExpressionMammalianMutationDeleted amino acid residues 316-405 in α -catenin…PromoterCMVAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 NSP3 Cys857Ala_nostop
Plasmid#154029PurposeGateway-compatible Entry vectorDepositorInsertNSP3
UseGateway-compatible entry vector, with mutagenized…MutationMany synonymous changes due to codon optimizationAvailable SinceJune 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21a-Med25-VBD-6His
Plasmid#64773PurposeBacterial expression of His-tagged Med25-VBDDepositorAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mTurquoise2-Puro
Plasmid#169224PurposeTargeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locusDepositorInsertDouble SapI flanked mTurquoise2-P2A-Puro
UseTargeting vector backboneAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL2-Mcl-1 mut
Plasmid#19133DepositorInsertMcl-1 promoter (MCL1 Human)
UseLuciferaseExpressionMammalianMutationNFkB binding-site mutant, the sequence GGGGTCTTCC…Available SinceOct. 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 sgKEAP1-1
Plasmid#74985PurposesgKEAP1-1, puromycin selectionDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF692
Plasmid#88701PurposeDonor Vector containing ZNF692 transcription factor, part of the Human TFome CollectionDepositorInsertZNF692 (ZNF692 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Gata3
Plasmid#128824Purposedox-dependent expression of mouse Gata3DepositorInsertGata3 (Gata3 Mouse)
UseLentiviralAvailable SinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXD13-IDR-mCherry-CRY2-NLS
Plasmid#145260PurposeMammalian Expression of Nuclear Opto HOXD13 IDR with SV40 NLSDepositorInsertHOXD13wt-IDR (HOXA13 Human)
ExpressionMammalianAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-FKO (NC-flag)
Plasmid#159363PurposeMammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.DepositorInsertSARS-CoV-2 S protein (S SARS-CoV-2)
TagsFlag and FlagExpressionMammalianMutationFurin-cleavage site mutationPromoterCMVAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Eomes
Plasmid#128825Purposedox-dependent expression of mouse EomesDepositorAvailable SinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-G614 (NC-flag)
Plasmid#156419PurposeMammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorInsertSARS-CoV-2 S protein (S SARS CoV2)
TagsFlagExpressionMammalianMutationD614GPromoterCMVAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV2 TEV-E
Plasmid#154418PurposeGateway-compatible Entry vectorDepositorInsertE (E SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of e…MutationMany synonymous changes due to codon optimizationAvailable SinceJuly 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAGE2-TetO-hSOX2
Plasmid#206388PurposeExpresses human SOX2 in mammalian cells, for lentivirus generation.DepositorInsertSOX2 (SOX2 Human)
UseLentiviralAvailable SinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF770
Plasmid#88562PurposeDonor Vector containing ZNF770 transcription factor, part of the Human TFome CollectionDepositorInsertZNF770 (ZNF770 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV2 TEV-M
Plasmid#154419PurposeGateway-compatible Entry vectorDepositorInsertM (M SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of m…MutationMany synonymous changes due to codon optimizationAvailable SinceJuly 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-Ef1a-Coff/Fon-sRGECO
Plasmid#137128PurposeIntersectional viral expression of sRGECO in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-sRGECO
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationE217DPromoterEF1aAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dCas9_sgRNA NC
Plasmid#176259PurposepNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI andDepositorInsertdead spCas9
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseMutationH840A and D10A mutations on spCas9 to inactivate …Available SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Myc-Nix-delta TM
Plasmid#100755PurposeMammalian expression of Nix dominant negativeDepositorAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF768
Plasmid#88524PurposeDonor Vector containing ZNF768 transcription factor, part of the Human TFome CollectionDepositorInsertZNF768 (ZNF768 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HOXD13(+10A)-IDR-mCherry
Plasmid#145282PurposeBacterial Expression of 6xHis HOXD13 (+10A) IDR mCherry Fusion ProteinDepositorAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon/Von GCaMP 6m
Plasmid#137165PurposeIntersectional viral expression of GCaMP6M in cells expressing Cre AND Flp AND VcreDepositorInsert3x-GCaMP6M
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationRemoved RSET tagPromoterEf1aAvailable SinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon-bREACHes-EYFP
Plasmid#137145PurposeIntersectional viral expression of bREACHes-EYFP in cells expressing both Cre AND FlpDepositorInsertCon/Fon-bREACHes-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationn/aPromoternEFAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG-CLTX CD32a ITAM CAR
Plasmid#171964PurposeDonor plasmid for CLTX CD32a-ITAM CAR expression in human cellsDepositorInsertSynthetic CLTX CD32a ITAM CAR
UseCRISPR, Synthetic Biology, and TALENExpressionMammalianPromoterCAGAvailable SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG-CLTX CD32a ITAM CD3z CAR
Plasmid#171965PurposeDonor plasmid for CLTX CD32a-ITAM CD3z CAR expression in human cellsDepositorInsertSynthetic CLTX CD32a ITAM CD3z CAR
UseCRISPR, Synthetic Biology, and TALENExpressionMammalianPromoterCAGAvailable SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DST-1/1
Plasmid#91288PurposeProtein expression and purification of human SH3 domain construct DST-1/1DepositorInsertDST-1/1 (DST Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)
Plasmid#36881DepositorInsertsGFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
Tags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available SinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STINGHAQ V155M
Plasmid#102592PurposeLentivector to express STING V155M HAQ and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralExpressionMammalianMutationV155M R71H-G230A-R293QPromoterSFFVAvailable SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Myc-Nix-MaoB
Plasmid#100757PurposeMammalian expression of mitochondria targetted NixDepositorInsertNix-MaoB (BNIP3L Human)
TagsMycExpressionMammalianMutationTM domain replaced by MaoBPromoterCMVAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_CRF1.0
Plasmid#208662PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent mannerDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
UseAAVPromoterEFSAvailable SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free GFP-NES
Plasmid#182491PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
GFP-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10 and GAL7Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only