We narrowed to 5,097 results for: puc

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AA198

Plasmid

#215985

Purpose

Fragmid fragment: (N' terminus) activator from p65 subunit of mouse Nfkb

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1; p65_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA277

Plasmid

#215999

Purpose

Fragmid fragment: (Selection) neomycin resistance

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; NeoR_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA081

Plasmid

#215977

Purpose

Fragmid fragment: (N' terminus) HIV Nuclear Export Sequence

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; HIV-NES_v1.2; HIV-NES_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA124

Plasmid

#215981

Purpose

Fragmid fragment: (N' terminus) catalytic core of human acetyltransferase p300

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; p300_v1.2; Linker_v3.1; SV40NLS_v1.6

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA141

Plasmid

#216045

Purpose

Fragmid fragment: (C' terminus) SV40NLS; Linker; p300

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

SV40NLS_v1.7; Linker_v4.1; p300_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA122

Plasmid

#215980

Purpose

Fragmid fragment: (N' terminus) 4xVP16 transcritional activators

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; VP64_v1.1; Linker_v3.1; SV40NLS_v1.6

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA091

Plasmid

#215979

Purpose

Fragmid fragment: (N' terminus) ZNF10 KRAB

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1; KRAB_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AA088

Plasmid

#215978

Purpose

Fragmid fragment: (N' terminus) ZIM3 KRAB

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1; KRAB_v2.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AA095

Plasmid

#216043

Purpose

Fragmid fragment: (C' terminus) ZNF10 KRAB

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

NpNLS_v1.1; KRAB_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA428

Plasmid

#216055

Purpose

Fragmid fragment: (C' terminus) NpNLS; 3xHA; c-mycNLS; c-mycNLS

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

NpNLS_v1.1; 3xHA_v1.1; c-mycNLS_v1.2; c-mycNLS_v1.3

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA050

Plasmid

#216059

Purpose

Fragmid fragment: (2A Selection) violet-excited GFP

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

P2A_v1.1; VexGFP_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA076

Plasmid

#216021

Purpose

Fragmid fragment: (Cas protein) Cas13d

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas13d_v1.1 [Rfx]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA370

Plasmid

#215988

Purpose

Fragmid fragment: (N' terminus) attenuate expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; u(S)ORF_v1.1; Stop; Start; BPNLS_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMamCol1

Plasmid

#215184

Purpose

Allows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coli

Depositor

Krishna Kishore Inampudi

Insert

tsPurple

Use

AAV, CRISPR, and Synthetic Biology ; Recombinant …

Tags

SNAC-StrepII tag

Expression

Bacterial and Mammalian

Promoter

TetON 3G Bidirectional for Mammalian and Trc with…

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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WT_IFL_A28C_R

Plasmid

#200184

Purpose

WT PA14 I-F Leader and A28C Repeat

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and a mutant I-F Locus (A28C)

Expression

Bacterial

Mutation

A28C in the I-F repeat

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b(Q119F/E475R/E758R)-T2A-EGFP

Plasmid

#188272

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

AaCas12b(Q119F/E475R/E758R)

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCA19-HSPB1 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#199450

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP harbors 12XMS2 in its intron) into the C-terminus of human HSPB1 gene

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCA22-SEC61B 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#199453

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP) into the C-terminus of human SEC61B gene

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCA20-HSPB1 5'HA-mTagBFP-3'-UTR 12XMS2V5-3'HA (HDR donor)

Plasmid

#199451

Purpose

CRISPR donor plasmid to insert [mTagBFP-stop-12XMS2] into the C-terminus of human HSPB1 gene; 12XMS2 locates in the 3' UTR region

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA21-HSPB1 5'HA-NarTag (12XPP7)-3'HA (HDR donor)

Plasmid

#199452

Purpose

CRISPR donor plasmid to insert NarTag (mTagBFP harbors 12XPP7 in its intron) into the C-terminus of human HSPB1 gene

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge