We narrowed to 5,495 results for: pcdna3.1

Showing: 4101 - 4120 of 5495 results

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pcDNA_RGG-GFP-RGG

Plasmid

#124930

Purpose

Expression of RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAB1679 CMV-HIVNES-GS-Cas13bt3

Plasmid

#176317

Purpose

CMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

human codon optimized Cas13bt3

Use

CRISPR

Tags

HIV NES

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pC3.1_CAG_oROS-Gr_LF(C199S)

Plasmid

#216114

Purpose

Expresses the loss-of-function version of the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.01.31.578117

Insert

oROS-Gr_LF(C199S)

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD1

Plasmid

#195839

Purpose

Expresses human FZD1 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD1 (FZD1 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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cyt-GafD-mTurboID-V5

Plasmid

#184640

Purpose

Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging

Depositor

Charlie Fehl

Article

Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

Insert

cyt-GlycoID-V5

Tags

Nuclear export sequence and V5 tag

Expression

Mammalian

Mutation

GafD -> expressed aa22-178 ; BirA* -> expre…

Promoter

CMV

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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HA-MCPIP1

Plasmid

#127459

Purpose

Expressing human MCPIP1 in mammalian cells

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTOPO-MCL1

Plasmid

#21605

Depositor

Ulrich Maurer

Article

Maurer et al Mol Cell. 2006 Mar 17. 21(6):749-60.

Insert

MCL-1 (MCL1 Human)

Expression

Mammalian

Mutation

Insert ends with a STOP codon, no V5-His-tag.

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA SF-HERC2 F4

Plasmid

#55787

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)

Tags

3xFLAG and STREP

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2014

Availability

Academic Institutions and Nonprofits only
pAL113

Plasmid

#22471

Purpose

Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PhyB NT (PHYB Mustard Weed, Mouse)

Use

Synthetic Biology

Tags

10aaLinker-mCFP-HrasCT

Expression

Mammalian

Mutation

Y276H

Promoter

CMV

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3-DCX-TurboID-V5

Plasmid

#190740

Purpose

Mammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

Depositor

Jeremy Baskin

Article

Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

Insert

doublecortin (DCX Human)

Tags

TurboID and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xFLAG-NLS-TPP1

Plasmid

#53585

Purpose

Mammalian expression of 3xFLAG tagged, nuclear localized TPP1, N-terminal.

Depositor

Steven Artandi

Article

Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

Insert

TPP1 (ACD Human)

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Silent mutation to eliminate PacI site

Promoter

CMV

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
mKO3-TEAD4

Plasmid

#221217

Purpose

RFP fused TEAD4

Depositor

Xiaokun Shu

Article

Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

Insert

mKO3-TEAD4 (TEAD4 )

Expression

Mammalian

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG HDAC5 WT

Plasmid

#32213

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA-p160MBP

Plasmid

#41

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Available Since

July 21, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA SF-HERC2 F5

Plasmid

#55788

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2 (3550-4450aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (3550-4450aa) (HERC2 Human)

Tags

3xFLAG and STREP

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2014

Availability

Academic Institutions and Nonprofits only
mSIRT3-L

Plasmid

#33309

Depositor

Michael Sack

Article

Bao et al J Cell Biochem. 2010 May;110(1):238-47.

Insert

Sirt3 (Sirt3 Mouse)

Tags

3XFlag

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

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nuc-GafD-mTurboID-HA

Plasmid

#184641

Purpose

Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging

Depositor

Charlie Fehl

Article

Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

Insert

nuc-GlycoID-HA

Tags

3xHA tag and Nuclear localization sequence

Expression

Mammalian

Mutation

GafD -> expressed 22-178 ; BirA* -> express…

Promoter

CMV

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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L2125-FRB-GPA-NLuc-CyOFP1

Plasmid

#222885

Purpose

Positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc-10GS-CyOFP1

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

Plasmid

#137827

Purpose

MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factor

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-CD28TMD-NTEVp(H75E)

Plasmid

#137829

Purpose

MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-CD28TMD-NTEVp(H75E)

Tags

3x FLAG

Expression

Mammalian

Mutation

Mutated Histidine 75 to Glutamic Acid in NTEVp fr…

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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