We narrowed to 13,228 results for: 109
-
Plasmid#171546PurposeEncoding part of the Drosophila Staufen protein from M571 to P656DepositorInsertStaufen (stau Fly)
UseTagsExpressionBacterialMutationa six amino acid_TRLTEG_insertion in the L1 regionPromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Staufen
Plasmid#171545PurposeEncoding part of the Drosophila Staufen protein from M571 to P656DepositorInsertStaufen (stau Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
4LNDD-pCDNA3.1(+)
Plasmid#172227PurposeExpresses human ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) in mammalian cellsDepositorInsertLambda N peptide - human ADAR2 Deaminase Domain
UseTags6xHis and FlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSN071
Plasmid#166729PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-Mxi1-NLS
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSN072
Plasmid#166730PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS-Mxi1-NLS
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK528
Plasmid#155388PurposedCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)DepositorInsertsdCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK434
Plasmid#155378PurposePphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)DepositorInsertsdCas9 (bacteria)
PA4-mVenus
PphlF_A4NT in dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)
Plasmid#149350PurposeMammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNADepositorInsertSpyCas9
UseCRISPRTagsFlag tagExpressionMammalianMutationPromoterCbhAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ad-HisFlag-USP19ER
Plasmid#155244Purposefor generating adenoviruses expressing the ER isoform of rat USP19DepositorInsertUSP19 (ER isoform) (Usp19 Rat)
UseAdenoviralTags6XHis-3XFlagExpressionMutationH118R- please see dep. commentsPromoterCMVAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEM323 - array co-injection fragment mosTI Pmlc-2_GFP
Plasmid#159836PurposeSingle copy or array insertion by MosTI using split Pmlc-2 GFP selectionDepositorInsertarray co-injection fragment mosTI Pmlc-2_GFP
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK422
Plasmid#155373PurposedCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)DepositorInsertsdCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK423
Plasmid#155374PurposedCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)DepositorInsertsdCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
AlkB-D135G
Plasmid#160434PurposeA plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to GlyDepositorInsertE. coli AlkB
UseTagsHis-tagExpressionBacterialMutationChanged Aspartic acid 135 to Glycine; deleted the…PromoterT7Available sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKH-1699
Plasmid#124227PurposeMammalian SpCas9-HF1 expressionDepositorInsertSpCas9-HF1
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-P1-N-mCherry-C
Plasmid#159437PurposeCRISPR knock-in donor construct with fluorescent reporterDepositorInsertmCherry
UseCRISPRTags3' linker pair 1, 5' linker pair 1, C t…ExpressionMutationPromoterCMVAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT215
Plasmid#134818PurposeSMT205 with DHFR-FLAG-tag, western blotDepositorInsertsDihydrofolate reductase
Thymidylate synthase
UseTagsFLAGExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT101
Plasmid#134815PurposeDual expression of DHFR and TYMS, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
UseTagsExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7Available sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA44
Plasmid#158522PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP (ZAR1 Mustard Weed)
UseTagseGFPExpressionPlantMutationL17E/D489VPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA16
Plasmid#158494PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V/L17E without STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationL17E, D481V, no STOP codonPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCVbsd-PPARD-K421R-K422R
Plasmid#130778PurposeVector encoding PPARD cDNA for retroviral transductionDepositorInsertPPARD K421R-K422R (PPARD Human)
UseRetroviralTagsExpressionMutationK421R-K422RPromoterAvailable sinceSept. 16, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCVbsd-PPARD-T423D-T427A
Plasmid#130792PurposeVector encoding PPARD cDNA for retroviral transductionDepositorInsertPPARD T423D-T427A (PPARD Human)
UseRetroviralTagsExpressionMutationT423D-T427APromoterAvailable sinceSept. 16, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZA7
Plasmid#158485PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E with STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationL17E, STOP codonPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA15
Plasmid#158493PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E without STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationL17E, no STOP codonPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA18
Plasmid#158496PurposeIn planta gene expression of Golden gate compatibleA. thaliana ZAR1 D489V. Km plant selection marker.DepositorInsertZAR1 (ZAR1 Mustard Weed)
UseTagsExpressionPlantMutationD489VPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA36
Plasmid#158514PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-6xHA. Km plant selection marker.DepositorInsertZAR1-6xHA (ZAR1 Mustard Weed)
UseTags6xHAExpressionPlantMutationL17E, D489VPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
IDA3::HA exon2 (WS13)
Plasmid#126874Purposep3xHA inserted within second exon of IDA3. Includes ampicilin and hygromycin resistance.DepositorInsertIDA3
UseOtherTagsExpressionMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
IDA3::NeonGreen N-term (WS14)
Plasmid#126875PurposeNeonGreen-tag attached to the N-terminus of IDA3. Includes ampicilin and hygromycin resistance.DepositorInsertIDA3
UseOtherTagsNeonGreenExpressionMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
IDA3::NeonGreen exon2 (WS15)
Plasmid#126876PurposeNeonGreen-tag embedded in the second exon of IDA3. Includes ampicilin and hygromycin resistance.DepositorInsertIDA3
UseOtherTagsExpressionMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA6
Plasmid#158484PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V with STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationD481V, STOP codonPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA14
Plasmid#158492PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V without STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationD481V, no STOP codonPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA19
Plasmid#158497PurposeIn planta gene expression of Golden gate compatlible A. thaliana ZAR1 L17E. Km plant selection marker.DepositorInsertZAR1 (ZAR1 Mustard Weed)
UseTagsExpressionPlantMutationL17EPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA35
Plasmid#158513PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-6xHA. Km plant selection marker.DepositorInsertZAR1-6xHA (ZAR1 Mustard Weed)
UseTags6xHAExpressionPlantMutationL17EPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA33
Plasmid#158511PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1-6xHA. Km plant selection marker.DepositorInsertZAR1-6xHA (ZAR1 Mustard Weed)
UseTags6xHAExpressionPlantMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA20
Plasmid#158498PurposeIn planta gene expression of Golden gate complatible A. thaliana ZAR1 D498V/L17E. Km plant selection marker.DepositorInsertZAR1 (ZAR1 Mustard Weed)
UseTagsExpressionPlantMutationL17E, D489VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA41
Plasmid#158519PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP (ZAR1 Mustard Weed)
UseTagseGFPExpressionPlantMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA43
Plasmid#158521PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP (ZAR1 Mustard Weed)
UseTagseGFPExpressionPlantMutationL17EPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA42
Plasmid#158520PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP (ZAR1 Mustard Weed)
UseTagseGFPExpressionPlantMutationD489VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA34
Plasmid#158512PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-6xHA. Km plant selection marker.DepositorInsertZAR1-6xHA (ZAR1 Mustard Weed)
UseTags6xHAExpressionPlantMutationD489VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA28
Plasmid#158506PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-4xMyc. Km plant selection marker.DepositorInsertZAR1-4xMyc (ZAR1 Mustard Weed)
UseTags4xMycExpressionPlantMutationL17E, D489VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA25
Plasmid#158503PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1-4xMyc. Km plant selection marker.DepositorInsertZAR1-4xMyc (ZAR1 Mustard Weed)
UseTags4xMycExpressionPlantMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT201
Plasmid#134816PurposeSMT101 with TET promoter for TYMS, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
UseTagsExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT2a_K30A
Plasmid#105694PurposeBacterial expression plasmids for production of recombinant metallothionein 2a K30A mutantDepositorInsertMetallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationLysine 30 to AlaninePromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT2a_K31A
Plasmid#105695PurposeBacterial expression plasmids for production of recombinant metallothionein 2a K31A mutantDepositorInsertMetallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationLysine 31 to AlaninePromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT2a_K30A K31A
Plasmid#105696PurposeBacterial expression plasmids for production of recombinant metallothionein 2a K30/31A mutantDepositorInsertMetallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationLysine 30 and 31 to AlaninePromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT2a_C21A
Plasmid#105697PurposeBacterial expression plasmids for production of recombinant metallothionein 2a C21A mutantDepositorInsertMetallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationCysteine 21 to AlaninePromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT2a_K20A
Plasmid#105698PurposeBacterial expression plasmids for production of recombinant metallothionein 2a K20A mutantDepositorInsertMetallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationLysine 20 to AlaninePromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT2a_S6A
Plasmid#105699PurposeBacterial expression plasmids for production of recombinant metallothionein 2a S6A mutantDepositorInsertMetallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationSerine 6 to AlaninePromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
pLenti-FNLSHF1-P2A-Puro
Plasmid#136901PurposeLentivirus for constitutive expression of FNLSHF1 in mammalian cells (codon optimized)DepositorInsertFNLSHF1
UseLentiviralTagsFLAG-NLS and NLSExpressionMutationR661A,Q695A,Q926APromoterEF1sAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCVbsd-PPARD-K421A-K422A
Plasmid#130777PurposeVector encoding PPARD cDNA for retroviral transductionDepositorInsertPPARD K421A-K422A (PPARD Human)
UseRetroviralTagsExpressionMutationK421A-K422APromoterAvailable sinceOct. 18, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits