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Addgene

We narrowed to 13,228 results for: 109

Showing: 10251 - 10300 of 13228 results
  1. pCSN071

    Plasmid
    #166729
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Insert
    dCas12a-Mxi1-NLS
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pCSN072

    Plasmid
    #166730
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Insert
    dCas12a-NLS-Mxi1-NLS
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pJK528

    Plasmid
    #155388
    Purpose
    dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)
    Depositor
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Promoter
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pJK434

    Plasmid
    #155378
    Purpose
    PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)
    Depositor
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pJK422

    Plasmid
    #155373
    Purpose
    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)
    Depositor
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    Available since
    Dec. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. pJK423

    Plasmid
    #155374
    Purpose
    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)
    Depositor
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    Available since
    Dec. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. AlkB-D135G

    Plasmid
    #160434
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly
    Depositor
    Insert
    E. coli AlkB
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Glycine; deleted the…
    Promoter
    T7
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. SMT215

    Plasmid
    #134818
    Purpose
    SMT205 with DHFR-FLAG-tag, western blot
    Depositor
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Use
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. pZA16

    Plasmid
    #158494
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V/L17E without STOP codon
    Depositor
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, D481V, no STOP codon
    Promoter
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. pZA7

    Plasmid
    #158485
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E with STOP codon
    Depositor
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, STOP codon
    Promoter
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. pZA15

    Plasmid
    #158493
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E without STOP codon
    Depositor
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, no STOP codon
    Promoter
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. pZA6

    Plasmid
    #158484
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V with STOP codon
    Depositor
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    D481V, STOP codon
    Promoter
    Available since
    Aug. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
  13. pZA14

    Plasmid
    #158492
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V without STOP codon
    Depositor
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    D481V, no STOP codon
    Promoter
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
  14. pZA28

    Plasmid
    #158506
    Purpose
    In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-4xMyc. Km plant selection marker.
    Depositor
    Insert
    ZAR1-4xMyc (ZAR1 Mustard Weed)
    Use
    Tags
    4xMyc
    Expression
    Plant
    Mutation
    L17E, D489V
    Promoter
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
  15. SMT201

    Plasmid
    #134816
    Purpose
    SMT101 with TET promoter for TYMS, in vivo assays
    Depositor
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
  16. MT2a_K30A

    Plasmid
    #105694
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 2a K30A mutant
    Depositor
    Insert
    Metallothionein 2a (MT2A Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Lysine 30 to Alanine
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
  17. MT2a_K31A

    Plasmid
    #105695
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 2a K31A mutant
    Depositor
    Insert
    Metallothionein 2a (MT2A Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Lysine 31 to Alanine
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
  18. MT2a_C21A

    Plasmid
    #105697
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 2a C21A mutant
    Depositor
    Insert
    Metallothionein 2a (MT2A Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Cysteine 21 to Alanine
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
  19. MT2a_K20A

    Plasmid
    #105698
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 2a K20A mutant
    Depositor
    Insert
    Metallothionein 2a (MT2A Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Lysine 20 to Alanine
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
  20. MT2a_S6A

    Plasmid
    #105699
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 2a S6A mutant
    Depositor
    Insert
    Metallothionein 2a (MT2A Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Serine 6 to Alanine
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 10251 - 10300 of 13228 results