We narrowed to 4,307 results for: ARA-2

1. pMXS-IRES-Blast POLE1 C-ISCmut

   Plasmid

   #160804

   Purpose

   Express C-terminal ISC mutant POLE1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLE1 C-ISCmut (POLE Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mutation

   shPOLE_1 and shPOLE2 resistant, C2221S, C2224S, C…

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXS-IRES-Blast POLD1 ISCmut

   Plasmid

   #160806

   Purpose

   Express ISC mutant POLD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLD1 ISCmut (POLD1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Codon optimized C-terminal 230 amino acids, C1058…

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXS-IRES-Blast POLA1 ISCmut

   Plasmid

   #160808

   Purpose

   Express ISC mutant POLA1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLA1 ISCmut (POLA1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Codon optimized, C1354S, C1359S, C1377S, C1380S

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. GST-PS-Gads 1-155 GB1 Gads 261-330-Cys (XSB365)

   Plasmid

   #78507

   Purpose

   Construct for expressing Gads SH3, SH2, and SH3 domain in E. Coli, with a C-terminal Cys for labeling

   Depositor

   Ron Vale

   Article

   Su et al Science. 2016 Apr 29;352(6285):595-9. do

   Insert

   Gads SH3 SH2 SH3 domain (GRAP2 Human)
   

   Use

   Tags

   GST followed by a prescission protease claevage s…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFB-EPCP1

   Plasmid

   #226783

   Purpose

   Expression of Z-tagged LRRK2 RCKW using Baculoviruses

   Depositor

   Sebastian Mathea

   Article

   Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

   Insert

   Leucine-rich repeat kinase 2 (LRRK2 Human)
   

   Use

   Tags

   His-Z-TEV

   Expression

   Insect

   Mutation

   Amino acids deleted 1-1326

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AICSDP-7:SEC61B-mEGFP

   Plasmid

   #87426

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61B

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   SEC61B Homology Arms with mEGFP-linker (SEC61B Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. AICSDP-10:LMNB1-mEGFP

   Plasmid

   #87422

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   LMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. AICSDP-8:TOMM20-mEGFP

   Plasmid

   #87423

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. AICSDP-4:TUBA1B-mEGFP

   Plasmid

   #87421

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1B

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   TUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. AICSDP-9:DSP-mEGFP

    Plasmid

    #87424

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human DSP

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    DSP Homology Arms with linker-mEGFP (DSP Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pF1-Hs Cdk1/cyclin B1

    Plasmid

    #177330

    Purpose

    Co-expression of the human active Cdk1/cyclin B1 kinase complex

    Depositor

    Andreas Boland

    Article

    Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

    Inserts

    Cyclin-dependent kinase 1 (CDK1 Human)
    G2/mitotic-specific cyclin-B1 (CCNB1 Human)
    

    Use

    Tags

    2 x Strep-tag and 8 x His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AICSDP-49: MYL2-mEGFP

    Plasmid

    #114414

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL2, via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AICSDP-13:FBL-mEGFP

    Plasmid

    #87427

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human FBL

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    FBL Homology Arms with linker-mEGFP (FBL Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pMXS-IRES-BLAST flag-CAD hCPS1 AD

    Plasmid

    #188130

    Purpose

    Expresses C-terminal flag-tagged human CAD hCPS1 AD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVZ_PcoaT_SQE1

    Plasmid

    #103860

    Purpose

    Heterologous, cobalt-inducible expression of SQE1 from A. thaliana in Synechocystis sp. PCC6803

    Depositor

    Ilka Axmann

    Article

    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d

    Insert

    squalene epoxidase 1 (XF1 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    inserted gene is codon optimized. 2nd amino acid …

    Promoter

    PcoaT (from Synechocystis sp. PCC 6803)

    Available since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. MKC89_CCR5(UbC-tEPOR-2A-YFP)

    Plasmid

    #232404

    Purpose

    AAV production plasmid for UbC-tEPOR-2A-YFP vector from Fig. 2 that mediates HDR at CCR5 locus using CCR5-sg3 gRNA. YFP is followed by a BGH polyA. AAV genome is flanked by AAV2 ITRs.

    Depositor

    Kyle Cromer, Matthew Porteus

    Article

    Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552

    Insert

    Truncated Erythropoietin Receptor (EPOR Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC368: pAAV.CMV-Cas13e-VEGFA sgRNA2

    Plasmid

    #227800

    Purpose

    Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA2

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-VEGFA sgRNA 2 (Vegfa Synthetic)
    Cas13e
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMXS-IRES-BLAST flag-CAD delta Loop1 and Loop2

    Plasmid

    #169833

    Purpose

    Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 and F1366 - P1376 in allosteric domain

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Deletions of amino acids R1398 - S1407 and F1366 …

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXS-IRES-BLAST flag-CAD delta Allosteric Domain

    Plasmid

    #169835

    Purpose

    Expresses C-terminal flag-tagged CAD with substitution of allosteric domain F1308 - C1455 with a (GGGS)X3 linker

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Substitution of amino acids F1308 - C1455 with a …

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXS-IRES-BLAST flag-CAD 3R-3E

    Plasmid

    #188148

    Purpose

    Expresses C-terminal flag-tagged human CAD 3R-3E in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge