We narrowed to 143,011 results for: ins

1. Sirius/pRSETB

   Plasmid

   #51956

   Purpose

   bacterial expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent protein

   Depositor

   Takeharu Nagai

   Article

   Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

   Insert

   Sirius
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRSETA-PAGFP

   Plasmid

   #11911

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

   Insert

   pRSETA-PAGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The PAGFP has four mutations (L64F/T65S/V163A/T20…

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDonor_Tn[sfGFP]

   Plasmid

   #213902

   Purpose

   Mariner transposon expressing sfGFP with kan selection casette

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with kan resistance and superfolder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. V5-BS2-NLS (Nucleoplasmin)

   Plasmid

   #220342

   Purpose

   Mammalian expression vector BS2 in nucleus

   Depositor

   Bryan Dickinson

   Article

   Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

   Insert

   BS2
   

   Use

   Tags

   NLS (Nucleus localization sequence) and V5 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. V5-BS2-NES (Cytosol)

   Plasmid

   #220341

   Purpose

   Mammalian expression vector BS2 in cytoplasm

   Depositor

   Bryan Dickinson

   Article

   Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

   Insert

   BS2
   

   Use

   Tags

   NES (Nuclear exclusion sequence) and V5 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. PKC delta DN

   Plasmid

   #16389

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC delta isoform 2 (Prkcd Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Dominant negative (kinase inactive): K376R

   Promoter

   Available since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AICSDP-82:SON-mEGFP

   Plasmid

   #133964

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human SON

   Depositor

   Allen Institute for Cell Science

   Insert

   SON Homology Arms with mEGFP-linker (SON Human)
   

   Use

   CRISPR

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Nov. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. KW78_HART_ODC1_PuroR

   Plasmid

   #207401

   Purpose

   Lentiviral plasmid containing DDX3X reporter HART for ODC1 5UTR - PuroR

   Depositor

   Stephen Floor

   Article

   Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 1

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mini CMV

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. V5-BS2-NIK3x (Nucleolus)

   Plasmid

   #220346

   Purpose

   Mammalian expression vector BS2 in nucleolus

   Depositor

   Bryan Dickinson

   Article

   Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

   Insert

   BS2
   

   Use

   Tags

   Nik3x (Nucleolus localization tag) and V5 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. PKC delta CAT

    Plasmid

    #16388

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC delta (Prkcd Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    CAT: catalytic domain, aa 334-674

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVITRO1-SS-338

    Plasmid

    #68795

    Purpose

    Single construct Sensor pair 1, ZF9 Op x6 -- GFP, with mCherry marker

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Inserts

    C-Terminal Sensor 1 V1
    mCherry
    ZF9 Op x6
    EGFP
    N-Terminal Sensor 1 V2
    

    Use

    Tags

    IRES, NLS, flag, and nls

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 3xF-NHP2

    Plasmid

    #126871

    Purpose

    Expresses 3xFlag NHP2

    Depositor

    Kathleen Collins

    Article

    Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

    Insert

    NHP2 (NHP2 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PKC beta II DN

    Plasmid

    #16385

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC beta II (Prkcb Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Dominant negative (kinase inactive): K371R

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PKC alpha dNPS

    Plasmid

    #21233

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC alpha (PRKCA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta 1-29

    Promoter

    Available since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

15. Mito-V5-BS2 (Mitochondrial matrix)

    Plasmid

    #220344

    Purpose

    Mammalian expression vector BS2 in mitocondria

    Depositor

    Bryan Dickinson

    Article

    Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

    Insert

    BS2
    

    Use

    Tags

    MTS-matrix and V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. AICSDP-116:PCNA-mEGFP

    Plasmid

    #170818

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human PCNA

    Depositor

    Allen Institute for Cell Science

    Insert

    PCNA Homology Arms with mEGFP-linker (PCNA Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    June 11, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. Filamin-C-stFRET

    Plasmid

    #61107

    Purpose

    Expression of Filamin with C-term stFRET inserted to measure mechanical stress in real time

    Depositor

    Fred Sachs

    Article

    Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

    Insert

    filamin-stFRET
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCIS-krPAM1

    Plasmid

    #25456

    Depositor

    Richard Mains

    Article

    Milgram et al J Cell Biol. 1992 May . 117(4):717-28.

    Insert

    PAM1 (Pam Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pET28b-miniR-DBCO

    Plasmid

    #181984

    Purpose

    Used to label proteins with the MiniE genetically encoded tag

    Depositor

    Kimberly Beatty

    Article

    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

    Insert

    MiniR-DBCO
    

    Use

    Tags

    DBCO-Tag, His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PSUPER-344(hPot1)

    Plasmid

    #11133

    Depositor

    Christopher Counter

    Article

    Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

    Insert

    RNAi against human Protection of Telomeres 1 (POT1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only