We narrowed to 11,993 results for: pcdna

1. pcDNA3.1 IGF1R-BirA(R118G)-HA

   Plasmid

   #109232

   Purpose

   This construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).

   Depositor

   Victor Dzau

   Article

   Bareja et al J Biol Chem. 2018 Mar 12. pii: RA118.002

   Insert

   IGF1R (IGF1R Human)
   

   Use

   Tags

   BirA(R118G) (highly promiscuous form) and HA

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA 3.2/V5-DEST CDC42EP1 WT

   Plasmid

   #69819

   Purpose

   Expresses CDC42EP1-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   CDC42 effector protein (CDC42EP1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-hAsCpf1(TYCV) (pY210)

   Plasmid

   #89351

   Purpose

   Expresses humanized AsCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K607R

   Promoter

   CMV

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO HIS HSPA1A

   Plasmid

   #19537

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   heat shock 70kDa protein 1A (HSPA1A Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.4 human alpha5 full

   Plasmid

   #221396

   Purpose

   Mammalian expression of human integrin alpha5 full length

   Depositor

   Timothy Springer

   Article

   Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

   Insert

   integrin alpha5 full length (ITGA5 Human)
   

   Use

   Tags

   CD33 secretion peptide (MPLLLLLPLLWAGALA) and St…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-CMV-mCherry-hPMCA2w/b

   Plasmid

   #111570

   Purpose

   Expresses a plasma membrane calcium pump hPMCA2w/b

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Insert

   Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3-HA-HA-human CMYC S62A

   Plasmid

   #203436

   Purpose

   Mammalian expression of MYC

   Depositor

   Yoshiaki Ito

   Article

   Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

   Insert

   MYC (MYC Human)
   

   Use

   Tags

   2 x HA

   Expression

   Mammalian

   Mutation

   S62A

   Promoter

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1+ hnRNPK myc HIS

   Plasmid

   #71662

   Purpose

   Mammalian expression of hnRNPK

   Depositor

   Gideon Dreyfuss

   Insert

   hnRNPK (HNRNPK Human)
   

   Use

   Tags

   HIS and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA5/FRT_CMV_NES-Kinprola_JNK-mEGFP

   Plasmid

   #233359

   Purpose

   CMV driven expression of the JNK activity recorder Kinprola_JNK in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

   Insert

   NES-Kinprola_JNK-mEGFP
   

   Use

   Tags

   NES and mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA5/FRT_CMV_NES-Kinprola_AMPK-mEGFP

    Plasmid

    #233361

    Purpose

    CMV driven expression of the AMPK activity recorder Kinprola_AMPK in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    NES-Kinprola_AMPK-mEGFP
    

    Use

    Tags

    NES and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA5/FRT_CMV_NES-Kinprola_PKC-mEGFP

    Plasmid

    #233357

    Purpose

    CMV driven expression of the PKC activity recorder Kinprola_PKC in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    NES-Kinprola_PKC-mEGFP
    

    Use

    Tags

    NES and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO HIS HSPA8

    Plasmid

    #19541

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 8 (HSPA8 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA4/TO/Strep-HA-ZAK alpha

    Plasmid

    #141193

    Purpose

    Expresses Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 (MAP3K20 Human)
    

    Use

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-TFEBS122D-MYC (#777)

    Plasmid

    #99956

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

    Insert

    TFEB (TFEB Human)
    

    Use

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    S122D

    Promoter

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4TO-mito-mCherry-10xGCN4_v4

    Plasmid

    #60914

    Purpose

    A fusion protein of the SunTag10xGCN4 v4 peptide array to a mitochondrial targeting domain and mCherry

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    mito-mCherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 2xFLAG-2xSTREP E2F7

    Plasmid

    #236442

    Purpose

    transient overexpression of E2F7 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    FLAG-FLAG-STREP-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO GFP HSPH1

    Plasmid

    #19480

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPH1 (HSPH1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)

    Plasmid

    #49077

    Purpose

    Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x HA and mCerulean

    Expression

    Mammalian

    Mutation

    262 silent mutations from native hNKCC1

    Promoter

    CMV

    Available Since

    Nov. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-6Xhis-human SUMO 3

    Plasmid

    #133772

    Purpose

    express 6Xhis- SUMO 3 protein

    Depositor

    Martine Roussel

    Article

    Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

    Insert

    SUMO 3 (SUMO3 Human)
    

    Use

    Tags

    6XHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. 1481 pcDNA3 flag HA 14-3-3 zeta

    Plasmid

    #9002

    Depositor

    William Sellers

    Insert

    14-3-3 zeta (YWHAZ Bovine)
    

    Use

    Tags

    HA and flag

    Expression

    Mammalian

    Mutation

    T226A

    Promoter

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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