We narrowed to 58,468 results for: tra

1. pKIF1C-GFP

   Plasmid

   #130977

   Purpose

   Expression of KIF1C-GFP in mammalian cells.

   Depositor

   Anne Straube

   Article

   Efimova et al J Cell Sci. 2014 Dec 15;127(Pt 24):5179-

   Insert

   KIF1C-GFP (KIF1C Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   5 silent mutations that make this construct RNAi …

   Promoter

   CMV

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKGmDUET-NgBR/hCIT

   Plasmid

   #203173

   Purpose

   Expression of human cis-prenyltransferase complex for purification

   Depositor

   William Sessa

   Article

   Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

   Inserts

   NUS1 (NUS1 Human)
   DHDDS (DHDDS Human)
   

   Tags

   Strep-Tag II-TEV and internally 6HIS tagged

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF0917

   Plasmid

   #142198

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NCOA3 (NCOA3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-mTagBFP2-rMPC1 shRNA

   Plasmid

   #229016

   Purpose

   Expression of an shRNA construct that knocks down rat MPC1. This plasmid also encodes a blue fluorescent protein tag to verify transfection

   Depositor

   Ghazaleh Ashrafi

   Article

   Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

   Insert

   MPC1 (Mpc1 Rat)
   

   Use

   Lentiviral

   Tags

   mTagBFP2

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFAST-BAC BAF155/SMARCC1-His

   Plasmid

   #177863

   Purpose

   Transfer vector to generate recombinant baculovirus expressing BAF155/SMARCC1-His

   Depositor

   Yoshihiro Izumiya

   Article

   Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

   Insert

   SMARCC1 (SMARCC1 Human)
   

   Tags

   His and His tag at C-terminus with GGGGS linker

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF3052

   Plasmid

   #144528

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NCOA3 (NCOA3 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-hARΔ142–337

   Plasmid

   #89106

   Purpose

   Mammalian expression of human androgen receptor with deletion of amino acids 142-337 N-terminal transactivation function 1

   Depositor

   Elizabeth Wilson

   Article

   Titus et al PLoS One. 2012;7(1):e30192. doi: 10.1371

   Insert

   hAR delta 142-337 (AR Human)
   

   Expression

   Mammalian

   Mutation

   hAR delta 142-337

   Promoter

   CMV

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

8. IC65 pEGFPN1 anti-IRSp53 nanobody

   Plasmid

   #135227

   Purpose

   Mammalian expression of anti-IRSp53 (Human) recombinant llama nanobody for use as an intrabody

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-IRSp53 (Human) recombinant llama nanobody IC65 (BAIAP2 L. glama (llama))
   

   Tags

   6xHis-HA-EGFP

   Expression

   Mammalian

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-Flt3-ITD-Y591F/Y919F

   Plasmid

   #74499

   Purpose

   Retroviral expression of FLT3 ITD-Y591F/Y919F

   Depositor

   Lars Rönnstrand

   Article

   Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

   Insert

   FLT3 (FLT3 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Y591F/Y919F, T227M

   Promoter

   unknown

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTwist-SARS-CoV-2 Δ18

    Plasmid

    #164436

    Purpose

    Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    SARS-CoV-2 Spike (S )
    

    Expression

    Mammalian

    Available Since

    Feb. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. DENV4 Rluc Reporter GND mutant Replicon

    Plasmid

    #118586

    Purpose

    For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.

    Depositor

    Radhakrishnan Padmanabhan

    Article

    Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.

    Insert

    DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
    

    Use

    Yeast-e. col shuttle vector prs424

    Mutation

    The conserved GDD in the NS5 polymerase gene muta…

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMega-MaFRSA

    Plasmid

    #200226

    Purpose

    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..

    Depositor

    Alanna Schepartz

    Article

    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

    Inserts

    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    mutated Asparagine 166 to Alanine and Valine 168 …

    Promoter

    proK-lacO and tacI

    Available Since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSB123 - pL0_rLUC-I (CDS1)

    Plasmid

    #123181

    Purpose

    Golden Gate (MoClo; CDS1) compatible luciferase gene from Renilla reniformis with an intron for transient and stable expression in plants

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    luciferase gene from Renilla reniformis with an plant intron and codon optimized for plants
    

    Use

    Luciferase; Part for plant expression

    Expression

    Plant

    Mutation

    No BpiI and BsaI sites; codon-optimised for plants

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Gfp-zf-vasa

    Plasmid

    #167323

    Purpose

    Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.

    Depositor

    Goro Yoshizaki

    Article

    Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

    Insert

    zf vasa 3'UTRs (ddx4 Zebrafish)
    

    Expression

    Bacterial

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHDM-SARS-CoV-2 Δ18

    Plasmid

    #164434

    Purpose

    Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    SARS-CoV-2 Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. TAPBPL pmRFP-N2 (721)

    Plasmid

    #62000

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    TAPBPL (TAPBPL Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-UbC-tdTomato

    Plasmid

    #62516

    Purpose

    Expresses tdTomato under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures

    Depositor

    Jae Lee, Pantelis Tsoulfas

    Article

    Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

    Insert

    tdTomato
    

    Use

    AAV and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-UbC-eGFP

    Plasmid

    #62518

    Purpose

    Expresses GFP under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures.

    Depositor

    Jae Lee, Pantelis Tsoulfas

    Article

    Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

    Insert

    eGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

    Plasmid

    #176534

    Purpose

    Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    CRISPR-associated IscB (Delaware Bay metagenome)
    

    Tags

    14xHis-MBP-bdSUMO and TwinStrep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEXP5-aHL_spytag_his

    Plasmid

    #107334

    Purpose

    Monomeric SpyTag-6×-His alpha-hemolysin

    Depositor

    George Church

    Article

    Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    alpha-hemolysin-SpyTag-6xHis
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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