162,536 results

1. pCMV-PEmax-P2A-GFP

   Plasmid

   #180020

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax-P2A-EGFP
   

   Use

   Tags

   P2A-EGFP, SV40 bpNLS, and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 FLAG FBXW7alpha

   Plasmid

   #236470

   Purpose

   transient overexpression of FBXW7alpha in mammalian cells

   Depositor

   Michele Pagano

   Article

   Busino et al Nat Cell Biol. 2012 Mar 4;14(4):375-85.

   Insert

   FBXW7alpha (FBXW7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBMN(CMV-copGFP-Puro-SMAR)

   Plasmid

   #80391

   Purpose

   expression copGFP and Puromycine, with an S/MAR element for episomal maintaince

   Depositor

   Magnus Essand

   Article

   Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

   Insert

   copGFP-T2A-Puromycine
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. CRISPR-StAR4B (Lenti)

   Plasmid

   #222691

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   PGK without BsmBI cutsite

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCX419

   Plasmid

   #229216

   Purpose

   All-in-one plasmid for labeling of XIST RNA via Csm complex

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   Csm1; Csm2, Csm3(RNase mut)-sfGFP; Csm4; Csm5; Cas6; XIST crRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+) mCherry

   Plasmid

   #176301

   Purpose

   Expresses mCherry mRNA from CMV promoter

   Depositor

   Jeremy Wilusz

   Article

   Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. ABE8e(TadA-8e V106W)

   Plasmid

   #138495

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e V106W)-nSpCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   TadA-8e V106W

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIRES Centrin1 GFP Pur

   Plasmid

   #64341

   Purpose

   Bicistronic expression of Centrin-GFP fusion and Puro resistance gene

   Depositor

   Matthieu Piel

   Insert

   Centrin1 (CETN1 Human)
   

   Use

   Tags

   GFP and IRES-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pT7-PE7 for IVT

   Plasmid

   #214813

   Purpose

   Template for in vitro transcription of PE7

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Template for in vitro transcription

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mutation

   Promoter

   T7 (inactivated)

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV R-CEPIA1er

    Plasmid

    #58216

    Purpose

    Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    R-CEPIA1er
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

    Promoter

    CMV

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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