We narrowed to 32,638 results for: CMV

1. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

   Plasmid

   #174137

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…

   Promoter

   CMV and T7

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-AAAAA-nCas9(D10A)-UGI

   Plasmid

   #187409

   Purpose

   DddA AAAAA variant

   Depositor

   Jin-Soo Kim

   Article

   Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

   Insert

   DddA AAAAA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

   Plasmid

   #185678

   Purpose

   Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERmembrane-HaloTag-KKMP
   

   Expression

   Mammalian

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p7137 pHAGE-P-CMVt-N-HA-GAW-EIF3M

   Plasmid

   #100149

   Purpose

   Expresses EIF3M

   Depositor

   Peter Howley

   Article

   Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

   Insert

   EIF3M (EIF3M Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2T-cmv-SpCas9-TadCBEa-BlastR

   Plasmid

   #193847

   Purpose

   Express TadCBEa in mammalian cells with BlastR selection, Tol2 integration

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadCBEa
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST-CMV 3xFLAG-LC3A-GFP

   Plasmid

   #123106

   Purpose

   Expresses 3xFLAG-LC3A-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3A

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
   

   Tags

   3xFLAG and EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-FLEX-SaCas9-U6-sgNts

   Plasmid

   #159909

   Purpose

   Mutagenesis of Nts

   Depositor

   Larry Zweifel

   Insert

   Nts (Nts Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. p7133 pHAGE-P-CMVt-N-HA-GAW-PRKAR2B

   Plasmid

   #100160

   Purpose

   Expresses PRKAR2B

   Depositor

   Peter Howley

   Article

   Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

   Insert

   PRKAR2B (PRKAR2B Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti CMV/TO V5-LUC Puro (w549-1)

   Plasmid

   #19785

   Purpose

   3rd gen lentiviral V5-Luciferase expression vector, CMV/TO promoter, Puro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   Firefly Luciferase
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-CMV 3xFLAG-GABARAP-GFP

    Plasmid

    #123111

    Purpose

    Expresses 3xFLAG-GABARAP-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAP

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
    

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV HA hRB delta CDK + T373

    Plasmid

    #58912

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with T373 restored

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pDEST-Tol2-PA2-CMV-AB-mCh

    Plasmid

    #160435

    Purpose

    Expresses human Amyloid Beta-mCherry in Zebrafish

    Depositor

    Nicholas Tolwinski

    Article

    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

    Insert

    Human Amyloid Beta peptide (1-42) (APP Human, Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    mCherry

    Promoter

    CMV

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

    Plasmid

    #159912

    Purpose

    Mutagenesis of Kcnd3

    Depositor

    Larry Zweifel

    Insert

    Kcnd3 (Kcnd3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-MCS-eDHFR(69K6)-EGFP

    Plasmid

    #172101

    Purpose

    Mammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    22aa-eDHFR(69K6)-EGFP
    

    Expression

    Mammalian

    Mutation

    eDHFR(69K6): Hexalysine (K6) sequence is inserted…

    Promoter

    CMV

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZac2.1 SV40-CMV-SaCas9-3xNLS

    Plasmid

    #78601

    Purpose

    AAV vector containing SaCas9

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    SV40-CMV-SaCas9-3xNLS
    

    Use

    AAV and CRISPR

    Tags

    HA tag

    Expression

    Mammalian

    Promoter

    SV40, CMV promoters

    Available Since

    Dec. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pLV-REPORT(PGK/CMV)-GtwyA

    Plasmid

    #208574

    Purpose

    LV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    mnucTomato
    HSVtk
    Neomycin
    d2nucEGFP
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    PGK/CMV and minP

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CMV-GFP-miRE_shSCR-Puro

    Plasmid

    #135689

    Purpose

    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Puromycin selection

    Depositor

    Florian Karreth

    Insert

    shScrambled
    

    Use

    Lentiviral

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PCMV-intron myc Rab11 S25N

    Plasmid

    #46786

    Purpose

    expression of S25N Rab11a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB11A S25N (RAB11A Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S25N dominant negative

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pLenti-CMV-tdTomato-miRE_shRen-Puro

    Plasmid

    #135687

    Purpose

    Constitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection

    Depositor

    Florian Karreth

    Insert

    shRenilla
    

    Use

    Lentiviral

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST-CMV 3xFLAG-GABARAPL1-GFP

    Plasmid

    #123112

    Purpose

    Expresses 3xFLAG-GABARAPL1-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL1

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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