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pGAPTrap-GFP

Plasmid

#82506

Purpose

Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH.

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc

Plasmid

#223559

Purpose

Alias: Gαi bONE-GO. Lentiviral vector expressing GINIP-Nluc after a low efficiency IRES downstream of YFP-CAAX placed after the promotor

Depositor

Mikel Garcia-Marcos

Article

Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457

Insert

YFP(Venus) - KRas4b - IRES* - human GINIP - linker(GGGS) - Nluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1_T6B -mCherry

Plasmid

#205600

Purpose

expresses human T6B peptide fused with mChery in mammalian cells

Depositor

Marc Friedlander

Article

Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

Insert

T6B peptide (fragment of human TRNC6B 599-683aa) (TNRC6B Human)

Tags

mCherry

Expression

Mammalian

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS1925

Plasmid

#105869

Purpose

High copy number plasmid containing iCreERT2 synthesized fragment for constructing the HPRT plasmid

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

iCreERT2

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Str-Endo-KKXX_SBP-EGFP-LIMP2

Plasmid

#222323

Purpose

Synchronize the trafficking of LIMP2 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LIMP2 fused to SBP-EGFP (SCARB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-CD28-mCherry

Plasmid

#223593

Purpose

Lentiviral expression of human CD28 with mCherry tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

mCherry

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-Neo-GFP/empty

Plasmid

#105505

Purpose

Empty backbone of MSCV-driven retroviral cDNA expression

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Type

Empty backbone

Use

Retroviral

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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p6XHis_NLS-SaCas9

Plasmid

#101086

Purpose

Express SaCas9 in bacteria with a 6xHis tag for purification

Depositor

Rick Tarleton

Article

Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

Insert

CRISPR-associated protein Cas9/Csn1

Use

CRISPR

Tags

TrxA-6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-PRDX1

Plasmid

#38086

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

PRDX1 peroxiredoxin 1 (PRDX1 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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PtPuc3_diaCas9_sgRNA

Plasmid

#109219

Purpose

Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum

Depositor

Per Winge

Article

Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10

Inserts

LHCF2 promoter
diaCas9
LHCF1 terminator
U6 promoter
sgRNA
U6 3' region

Use

CRISPR and Synthetic Biology

Promoter

LHCF1 terminator, LHCF2 promoter, U6 3' regi…

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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XLone-Puro Cas13d-eGFP U6 BbsI

Plasmid

#155184

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and U6 gRNA backbone

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Type

Empty backbone

Use

Unspecified; Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTCB-nivoHCLALAPGknobHA

Plasmid

#237377

Purpose

pTwist CMV BetaGlobin encoding nivolumab heavy chain with LALA P329G knob Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

nivolumab heavy chain (silent Fc)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-nivoLC

Plasmid

#237378

Purpose

pTwist CMV BetaGlobin encoding nivolumab light chain

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

nivolumab light chain

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.PHF19

Plasmid

#125168

Purpose

Expresses human PHF19 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

PHF19 (PHF19 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLX304-IRES-Td

Plasmid

#176296

Purpose

pLX304 backbone with an IRES-Tdtomato cassette subcloned into the NheI-AgeI sites

Depositor

William Kaelin

Article

Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

Type

Empty backbone

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX4T2 RANK-L

Plasmid

#108571

Purpose

Encodes C-terminal part of RANKL (aa158-aa316) with a GST N-terminal tag

Depositor

Jean-Claude Scimeca

Article

Beranger et al J Bone Miner Res. 2007 Jul;22(7):975-83.

Insert

RANKL (receptor activator of nuclear factor kappa-B ligand) (Tnfsf11 Mouse)

Tags

GST

Expression

Mammalian

Mutation

aa158-aa316

Promoter

Ptac (trp/lac)

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM2-Flag

Plasmid

#180407

Purpose

lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM2 (TGM2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTCB-avelHCknob6xHis

Plasmid

#237383

Purpose

pTwist CMV BetaGlobin encoding avelumab heavy chain with knob Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

avelumab heavy chain

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-avelLC

Plasmid

#237384

Purpose

pTwist CMV BetaGlobin encoding avelumab light chain

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

avelumab light chain

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_Ef1a:PE2-P2A-hMLH1dn

Plasmid

#184444

Purpose

Single EF1a-driven PE2-P2A-hMLH1dn (Addgene #174828) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

Depositor

Bobby Koeleman

Article

Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and Ef1A

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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