We narrowed to 143,011 results for: ins

1. CelTag Plasmid

   Plasmid

   #66562

   Purpose

   Encodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.

   Depositor

   David Engelke

   Article

   Carrick et al G3 (Bethesda). 2015 Dec 29. pii: g3.115.

   Insert

   CelTag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. PKC delta dNPS

   Plasmid

   #16387

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC delta (Prkcd Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N-terminal pseudosubstrate domain deletion: aa 15…

   Promoter

   Available since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

3. dSaCas9-KRAB

   Plasmid

   #205250

   Purpose

   In vitro transcription plasmid for production of dSaCas9-KOX1 KRAB mRNA for transcriptional activation (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #106219.

   Depositor

   Rasmus Bak

   Insert

   dSaCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPICZA_CeTRIC-B1_Del

   Plasmid

   #87344

   Purpose

   Inducible expression of CeTRIC-B1 in P.Pichia strains

   Depositor

   Zhenfeng Liu

   Article

   Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

   Insert

   TRIC-B1
   

   Use

   Tags

   polyhistidine

   Expression

   Yeast

   Mutation

   Δ248-297

   Promoter

   AOX1

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. UIMLx2

   Plasmid

   #174888

   Purpose

   UIMLx2 ubiquitin probe for K48 ubiquitylated proteins

   Depositor

   Peter Kaiser

   Article

   Villamil et al Mol Cell Proteomics. 2022 Jan;21(1):1001

   Insert

   Met4 UIML
   

   Use

   Tags

   Avi-6xHis-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PKC gamma CAT

   Plasmid

   #21238

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC gamma (Prkcg Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta 1-337

   Promoter

   Available since

   Aug. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Filamin-M-stFRET

   Plasmid

   #61105

   Purpose

   Expression of Filamin with internal stFRET inserted to measure mechanical stress in real time

   Depositor

   Fred Sachs

   Article

   Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

   Insert

   filamin-stFRET
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Actinin-M-stFRET

   Plasmid

   #61103

   Purpose

   Expression of α-actinin with internal stFRET inserted to measure mechanical stress in real time

   Depositor

   Fred Sachs

   Article

   Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

   Insert

   α-actinin-stFRET
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAGGS_2paLINX

   Plasmid

   #129655

   Purpose

   2P-activatable light-inducible nuclear exporter

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

   Insert

   2paLINX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Actinin-C-stFRET

    Plasmid

    #61104

    Purpose

    Expression of α-actinin with C-term stFRET inserted to measure mechanical stress in real time

    Depositor

    Fred Sachs

    Article

    Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

    Insert

    α-actinin-stFRET
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. JAF99.4

    Plasmid

    #45560

    Depositor

    Gregory Pazour

    Article

    Follit et al J Cell Biol. 2010 Jan 11;188(1):21-8. do

    Insert

    Pkhd1 (Pkhd1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Contains only the cytoplasmic tail (last 193 amin…

    Promoter

    CMV

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

12. PKC gamma dNPS

    Plasmid

    #21237

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC gamma (Prkcg Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta 1-28

    Promoter

    Available since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pAAH6

    Plasmid

    #41830

    Purpose

    Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for ClonNAT resistance

    Depositor

    Melissa Moore

    Article

    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

    Type

    Empty backbone

    Use

    Pcr template

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    T7,TEF

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC-EsaR-D91G-EsaI

    Plasmid

    #47662

    Purpose

    esaI gene downstream of esaR-a272g in pAC-EsaR-D91G (CmR)

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Synth Biol. 2013 Jul 23.

    Insert

    esaI-term
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pAC-EsaR-I70V-EsaI

    Plasmid

    #47661

    Purpose

    esaI gene downstream of esaR-a208g in pAC-EsaR-I70V (CmR)

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Synth Biol. 2013 Jul 23.

    Insert

    esaI-term
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pAc5 dOrai

    Plasmid

    #16368

    Depositor

    Anjana Rao

    Article

    Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

    Insert

    Orai (olf186-F Fly)
    

    Use

    Tags

    V5

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

17. Vh-HA-DHFR

    Plasmid

    #20217

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    DHFR (folA E. coli)
    

    Use

    Tags

    HA and Vh

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pAAH2

    Plasmid

    #41826

    Purpose

    Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for hygromycin resistance.

    Depositor

    Melissa Moore

    Article

    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

    Type

    Empty backbone

    Use

    Pcr template

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    T7, TEF

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVY-3 sfGFP-tag3

    Plasmid

    #194618

    Purpose

    fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

    Insert

    sfGFP-K(GGSKKRKKR)3
    

    Use

    Tags

    KGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTricHis-ILY-WT-Y336W

    Plasmid

    #160515

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-WT-Y336W
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Y336W

    Promoter

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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