We narrowed to 58,468 results for: tra

1. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

   Plasmid

   #171090

   Purpose

   CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Sth1 dCas9
   Chloramphenicol acetyltransferase
   TagBFP
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

   Mutation

   D9A, H599A

   Promoter

   TUB1 (Toxoplasma gondii), transcriptionally linke…

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SS80 pEGFPN1 anti-SAPAP2 nanobody

   Plasmid

   #135228

   Purpose

   Mammalian expression of anti-SAPAP (Rat) recombinant llama nanobody for use as an intrabody

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-SAPAP2 (Rat) recombinant llama nanobody SS80 (Dlgap2 L. glama (llama))
   

   Tags

   6xHis-HA-EGFP

   Expression

   Mammalian

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-Bcl2-Cb5

   Plasmid

   #18004

   Depositor

   Clark Distelhorst

   Article

   Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

   Insert

   ER targeted Bcl-2 (BCL2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C terminal of Bcl-2 replaced with Cytochrome b5. …

   Promoter

   CMV

   Available Since

   June 4, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-EF1a-FLuc-WPRE-HGHpA

   Plasmid

   #87951

   Purpose

   Recombinant single-stranded AAV transfer vector expressing Firefly Luciferase under the EF1a promoter

   Depositor

   Mark Kay

   Article

   Paulk et al Mol Ther. 2017 Sep 25. pii: S1525-0016(1

   Insert

   Firefly Luciferase
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag-Bcl2-Maob

   Plasmid

   #18005

   Depositor

   Clark Distelhorst

   Article

   Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

   Insert

   Mitochondria-targeted Bcl-2 (BCL2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C terminal of Bcl-2 replaced with Maob. Targets B…

   Promoter

   CMV

   Available Since

   June 4, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pCI-Puro_mouse_Adar2_Tv1

   Plasmid

   #158111

   Purpose

   expresses mADAR2 shortest transcript, puromycin selection

   Depositor

   Emanuela Felley-Bosco

   Article

   Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

   Insert

   adar2 (Adarb1 Mouse)
   

   Expression

   Mammalian

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSB63 - pL0_fLUC-I (CDS1)

   Plasmid

   #123180

   Purpose

   Golden Gate (MoClo; CDS1) compatible luciferase gene from Photinuspyralis with an intron for transient and stable expression in plants

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   Luciferase gene from Photinuspyralis with intron from U5 small nuclear ribonucleoprotein component (Arabidopsis thaliana)
   

   Use

   Luciferase; Part for plant expression

   Expression

   Plant

   Mutation

   No BpiI and BsaI sites

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer

   Plasmid

   #176589

   Purpose

   Endogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacer

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associate IscB (Chesapeake Bay) locus
   

   Expression

   Bacterial

   Mutation

   Truncated CRISPR array to two direct repeats, rep…

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Flt3-Y591F/Y919F

   Plasmid

   #74497

   Purpose

   Overexpression of FLT3 Y591F/Y919F mutant in mammalian cells

   Depositor

   Lars Rönnstrand

   Article

   Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

   Insert

   FLT3 (FLT3 Human)
   

   Expression

   Mammalian

   Mutation

   Y591F/Y919F, T227M

   Promoter

   CMV

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pTwist-SARS-CoV-2 Δ18 K417N+E484K+N501Y

    Plasmid

    #169469

    Purpose

    Encodes SARS-CoV-2 K417N+E484K+N501Y Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 K417N+E484K+N501Y Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. phBG-P53DD

    Plasmid

    #149707

    Purpose

    In vitro transcription of dominant negative form of P53 mRNA for RNA transfection into mammalian cells

    Depositor

    Erika Sasaki

    Article

    Watanabe et al Genes Cells. 2019 Jul;24(7):473-484. doi

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    Rna transcription

    Mutation

    deleted amino acid 14-301

    Promoter

    T7

    Available Since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-Flt3-Y591F/Y919F

    Plasmid

    #74498

    Purpose

    Retroviral expression of FLT3 Y591F/Y919F

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Y591F/Y919F, T227M

    Promoter

    unknown

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pTwist-SARS-CoV-2 Δ18 B.1.351v2/wtRBD

    Plasmid

    #169467

    Purpose

    Encodes SARS-CoV-2 B.1.351v2/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v2/wtRBD Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221_SLC36A2

    Plasmid

    #132072

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC36A2 (SLC36A2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pUAS-spatzle-HA

    Plasmid

    #240235

    Purpose

    Expression of spatzle-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    spatzle (spz Fly)
    

    Tags

    HA-spGFP11

    Expression

    Insect

    Promoter

    UAS

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. AP2u2-CMV-S_protein

    Plasmid

    #226456

    Purpose

    Mammalian expression of S protein of SARS-CoV2 under CMV promoter, tagged with 6His and released extracellular environment after expression for protein purification

    Depositor

    Urartu Seker

    Article

    Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(

    Insert

    Spike Protein
    

    Tags

    6xHis Tag, Foldon Domain, and Thrombin Cleavage S…

    Expression

    Mammalian

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF0916

    Plasmid

    #142238

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NCOA3 (NCOA3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid

    #177360

    Purpose

    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoter

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Inserts

    tdTomato
    Chimeric guide for SaCas9
    

    Use

    AAV

    Tags

    P2A and a tetracycline-controlled transactivator …

    Expression

    Mammalian

    Promoter

    Synapsine promoter and U6 promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTwist-SARS-CoV-2 Δ18 B.1.351v3/wtRBD

    Plasmid

    #169468

    Purpose

    Encodes SARS-CoV-2 B.1.351v3/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v3/wtRBD Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTwist-SARS-CoV-2 Δ18 B.1.351v1/wtRBD

    Plasmid

    #169466

    Purpose

    Encodes SARS-CoV-2 B.1.351v1/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v1/wtRBD Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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