We narrowed to 9,318 results for: Coli

1. B0032m_BC

   Plasmid

   #66020

   Purpose

   MoClo Basic Part: RBS - Weiss RBS, medium strength. Modified from Bba_B0032 to adjust spacing in MC system. [B:B0032m:C]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   RBS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJ02B2Gm_EF

   Plasmid

   #66064

   Purpose

   MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   FACS Controls
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM6_Ptrc_BsMdh_BmHps_BmPhi

   Plasmid

   #129104

   Purpose

   Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus

   Depositor

   Eleftherios Terry Papoutsakis

   Article

   Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

   Insert

   BsMDH BmHPS BmPHI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 11, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pFA-sfGFP

   Plasmid

   #162689

   Purpose

   pFA vector control expressing sfGFP

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   sfGFP
   

   Use

   Tags

   C terminal 6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   PL tet promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAL950

   Plasmid

   #119748

   Purpose

   precursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine

   Depositor

   Linda Randall

   Article

   Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

   Insert

   precusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Internal NdeI site removed and mutations Alanine …

   Promoter

   T7

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. SLIRP.HMG6.wt.fl

   Plasmid

   #49821

   Purpose

   expression vector for MBP-SLIRP fusion

   Depositor

   Thomas Cech

   Article

   McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

   Insert

   SLIRP SRA stem-loop interacting RNA binding protein (SLIRP Human)
   

   Use

   Tags

   E. coli Maltose Binding Protein

   Expression

   Bacterial

   Mutation

   wildtype codon-optimized for E. coli expression

   Promoter

   T7

   Available since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTTR210K

   Plasmid

   #41750

   Depositor

   Jeff Gelles

   Article

   Thoresen et al Biochemistry. 2008 Sep 9;47(36):9514-21.

   Insert

   kinesin heavy chain (Khc Fly, E. coli)
   

   Use

   Tags

   His6 and bccp

   Expression

   Bacterial

   Mutation

   residues 1-401; R210K

   Promoter

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pBEST_OR2-OR1-Pr_UTR1-deGFP-T500 BsaI, BbsI-safe (105p)

   Plasmid

   #70194

   Purpose

   Derived from 21 with silent mutations to make BsaI, BbsI compatible

   Depositor

   Richard Murray

   Article

   Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

   Insert

   deGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. 6his R.3-AR GUSpET28a+/pLysS

   Plasmid

   #61157

   Purpose

   N-terminal His tagged round 3 aldehyde-resistant GUS expression vector

   Depositor

   Ichiro Matsumura, Andrew Ellington

   Article

   Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

   Insert

   GUSAR
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   N66D, D151N, A219V, I396T, T480A, Q498R, D508E an…

   Promoter

   T7

   Available since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGE76

    Plasmid

    #80578

    Purpose

    recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:nptII-tmos
    p2x35S:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLaNGo11-3

    Plasmid

    #126054

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMiNGo11-1

    Plasmid

    #126056

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM6-Pc4CL-PhCHS-CmCHI

    Plasmid

    #73397

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    Pc4CL
    PhCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEM-Cas9noSsrA

    Plasmid

    #102285

    Purpose

    Modified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    removed ssrA tag

    Promoter

    pTet

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGE77

    Plasmid

    #80579

    Purpose

    recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:PAT-tnos
    p2x35S:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGE78

    Plasmid

    #80580

    Purpose

    recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:nptII-tmos
    pPcUbi:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGE79

    Plasmid

    #80581

    Purpose

    recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:PAT-tnos
    pPcUbi:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHL 1032

    Plasmid

    #52997

    Purpose

    pLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    RhyB sRNA
    ompC
    sodB
    

    Use

    Synthetic Biology

    Tags

    gfp and mCherry

    Expression

    Bacterial

    Mutation

    contains −56 to +141 bp relative to start codon a…

    Promoter

    pCon, pLlacO-1, and pLtetO-1

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pFP121

    Plasmid

    #21670

    Depositor

    James Haber

    Article

    Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

    Insert

    Lacz (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Tags

    Expression

    Mutation

    The second lacZ sequence has been modified, the E…

    Promoter

    Available since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pBN415

    Plasmid

    #217920

    Purpose

    express E. coli E69 (O9a:K30) Wzc with N711Y mutation

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2025 Apr 11;16(1):3437. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc with N711Y mutation
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    N711Y

    Promoter

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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