We narrowed to 29,541 results for: REP

1. pgRNA_CV2

   Plasmid

   #167165

   Purpose

   pgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.

   Depositor

   Alexander Loewer

   Article

   Sheng et al Cell Rep. 2019 Apr 2;27(1):48-58.e7. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Donor-PIGAshort

   Plasmid

   #153547

   Purpose

   Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   PIGA (a 603-bp fragment from intron 5 and exon 6)
   

   Use

   Donor plasmid

   Tags

   N/A

   Expression

   Mutation

   No mutation

   Promoter

   No promoter

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-Miro1 (184-618)

   Plasmid

   #127626

   Purpose

   Human Miro1 lacking the first GTPase domain

   Depositor

   Josef Kittler

   Article

   Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

   Insert

   Miro1 (RHOT1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   184-618 only

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-Miro1 (412-618)

   Plasmid

   #127628

   Purpose

   GTPase2 domain of human Miro1 including transmembrane domain and C-terminal amino acids

   Depositor

   Josef Kittler

   Article

   Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

   Insert

   Miro1 (RHOT1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   412-618 only

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAA 442TAG

   Plasmid

   #154778

   Purpose

   plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAA 277TAG, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   LaG17-SynNotch
   

   Use

   Tags

   LaG17

   Expression

   Mammalian

   Mutation

   204TAA 442TAG in LaG17-SynNotch, hybrid PylT with…

   Promoter

   EF1

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST40-2XHA-Src-Y530EEI

   Plasmid

   #140322

   Purpose

   Expresses HA-tagged human Src-Y530EEI in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Use

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   Y530EEI

   Promoter

   CMV

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPRIME-CMV-GFP-shNts2

   Plasmid

   #132714

   Purpose

   Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Nts

   Depositor

   Robert Messing

   Article

   Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

   Insert

   shNts(2) (Nts Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPRIME-CMV-GFP-shNts1

   Plasmid

   #132713

   Purpose

   Encodes short hairpin RNA (shRNA) #1 that targets the the 3’-untranslated region of the rat Nts gene

   Depositor

   Robert Messing

   Article

   Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

   Insert

   shNts(1) (Nts Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKI-cjPLP1e5-S253T

   Plasmid

   #129755

   Purpose

   Gene targeting in marmoset cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

   Insert

   PLP1 (PLP1 C. jacchus (common marmost))
   

   Use

   Marmoset targeting

   Tags

   Expression

   Mutation

   S253T

   Promoter

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKI-cjPLP1e5-P216S

    Plasmid

    #129754

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    PLP1 (PLP1 C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    Expression

    Mutation

    P216S

    Promoter

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKI-cjPLP1e2-P15L-_lox

    Plasmid

    #129756

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    PLP1 (PLP1 C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    Expression

    Mutation

    P15L

    Promoter

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKI-cjPLP1e1-EGFP

    Plasmid

    #129751

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    PLP1 (PLP1 C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKI-cjPLP1e2-A39T

    Plasmid

    #129752

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    PLP1 (PLP1 C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    Expression

    Mutation

    A39T

    Promoter

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKI-cjPLP1e2-P15L

    Plasmid

    #129753

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    PLP1 (PLP1 C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    Expression

    Mutation

    P15L

    Promoter

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. A3H HapII R175/6E-Cas9n-UGI

    Plasmid

    #119142

    Purpose

    Base editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EE

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3H Haplotype II RR175/6EE-Cas9 nickase-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    A3H Hap II RR175/6EE

    Promoter

    CMV

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCHR-mClover-smAID-P2A-CD5Flag2-bglpA

    Plasmid

    #110654

    Purpose

    Lentiviral vector expressing cell surface Flag-tag and monomeric GFP C-terminaly fused with auxin inducible degron

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    mClover-AID-P2A-CD5Flag2-bglpA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUCHR-mClover-smAID-P2A-CD5cmyc2-bglpA

    Plasmid

    #110656

    Purpose

    Lentiviral vector expressing cell surface c-Myc-tag and monomeric GFP C-terminaly fused with auxin inducible degron

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    mClover-AID-P2A-CD5cmyc2-bglpA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUCHR-CD5HA2-P2A-smAID-mClover

    Plasmid

    #110658

    Purpose

    Lentiviral vector expressing cell surface HA-tag and monomeric GFP N-terminaly fused with auxin inducible degron

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    CD5HA2-P2A-AID-mClover
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3.1(-) + SLNCR1 _1336-1496

    Plasmid

    #86823

    Purpose

    mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    LINC00673 (LINC00673 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3.1(-) + SLNCR1 _1521-2092

    Plasmid

    #86824

    Purpose

    mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    LINC00673 (LINC00673 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge