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pS23.U6<ActB.mus_C-term-KI_gRNA

Plasmid

#207408

Purpose

gRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]

Depositor

Alexandros Poulopoulos

Article

Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

Insert

ActB

Use

CRISPR and Mouse Targeting

Promoter

U6

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago2_4

Plasmid

#73532

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago2

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO2

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-NLS

Plasmid

#103820

Purpose

Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-NLS (CRY2 Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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HSF1 KO

Plasmid

#200207

Purpose

gRNA for HSF1 KO

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

HSF1 KO (Hsf1 Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_PRKCI

Plasmid

#45263

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PKCI (PRKCI Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

June 14, 2013

Availability

Academic Institutions and Nonprofits only
ama1CD4d3+4-blac

Plasmid

#36146

Purpose

EXPRESs plasmid for P. falciuparum merozoite surface proteins encoding P. falciparum ama1 with rat CD4d3+4-blac

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

ama1 (PF3D7_1133400 Plasmodium falciparum)

Tags

beta-lactamase, pentamerization domain of rat car…

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX SH2A-SH2

Plasmid

#46481

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2A (SH2D4A Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 338-445

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pEY61

Plasmid

#191058

Purpose

acr-2(3.7k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acr-2(3.7k) (acr-2 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shStk16-1

Plasmid

#180391

Purpose

Producing AAV that encodes mouse Stk16 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shStk16-1 (Stk16 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX Lnk-SH2

Plasmid

#46451

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Lnk (Sh2b3 Mouse)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 325-466

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Slap2-SH2

Plasmid

#46501

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Slap2 (SLA2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 84-197

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCDB181

Plasmid

#80692

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHEE_06

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEHEE_06

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pNAS1b split mCherry NEDD4-2 WW2

Plasmid

#111883

Purpose

Mode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Type

Empty backbone

Tags

Split mCherry system: NEDD4-2 WW2 fused to C-term…

Expression

Bacterial

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.mTagBFP2.let-7c

Plasmid

#169310

Purpose

Constitutive overexpression of let-7c with mTagBFP2 as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

let-7c (MIRLET7C Human)

Use

Lentiviral

Tags

TagBFP2

Promoter

SFFV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX p85b(NC)-SH2

Plasmid

#46468

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

p85b(NC) (PIK3R2 Bovine)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 311-717

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Nck2-SH2

Plasmid

#46458

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

NM_001004720.2 (NCK2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 274-380

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pSECB sgMTHFD2L_1

Plasmid

#106306

Purpose

Express Cas9 and sgRNA targeting MTHFD2L

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD2L (MTHFD2L Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pSECB sgMTHFD2L_2

Plasmid

#106307

Purpose

Express Cas9 and sgRNA targeting MTHFD2L

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD2L (MTHFD2L Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX Tem6-SH2

Plasmid

#46523

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Tem6 (TNS3 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1171-1291

Promoter

Tac

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Vrap-SH2

Plasmid

#46531

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Vrap (SH2D2A Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 93-192

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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