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Showing: 8321 - 8360 of 9862 results

  1. iDianiSnFR

    Plasmid
    #177741
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    iDianiSnFR
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177741-plasmid-map-sequence-id-348683

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  2. iCytSnFR

    Plasmid
    #177738
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    iCytSnFR
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    177738-plasmid-map-sequence-id-348679

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  3. iCyt_BrEt_SnFR

    Plasmid
    #177740
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    iCyt_BrEt_SnFR
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    177740-plasmid-map-sequence-id-349387

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  4. iCyt_F_SnFR

    Plasmid
    #177739
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    iCyt_F_SnFR
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    177739-plasmid-map-sequence-id-348682

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  6. pCCM’ CbbQ-

    Plasmid
    #162711
    Purpose
    Second CCM operon with inactive CbbQ (K46A, E107Q) rubisco activase
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    Second CCM operon cloned from H. neapolitanus
    Expression
    Bacterial
    Mutation
    Inactivation of CbbQ subunit of CbbOQ rubisco act…
    Promoter
    PLtet0-1 promoter
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162711-plasmid-map-sequence-id-324197

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  7. Tac(5A)-GFP

    Plasmid
    #162495
    Purpose
    Expresses C-terminally GFP tagged Tac with mutated five O-glycosylation sites in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    Tac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five threonine residues are mutated into alan…
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162495-plasmid-map-sequence-id-323475

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  8. GFP-Tac(5A)

    Plasmid
    #162493
    Purpose
    Expresses GFP tagged Tac with mutated five O-glycosylation sites in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    Tac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five threonine residues are mutated into alan…
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162493-plasmid-map-sequence-id-323471

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  9. pMinDis.X513-iNicSnFR3b-(V7)-ER

    Plasmid
    #125123
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine
    Depositor
    Henry Lester
    Article
    Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018
    Insert
    iNicSnFR3a-(V7)-ER
    Tags
    Fusion protein -Endoplasmic retention motif
    Expression
    Mammalian
    Promoter
    T7
    Available Since
    Oct. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    125123-plasmid-map-sequence-id-245436

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  14. PAK4-FL-3'UTR-MT2

    Plasmid
    #113093
    Purpose
    Luciferase reporter containing PAK4-FL- 3' UTR with the second predicted MiR-199a-3p binding site mutated
    Depositor
    James Donahue
    Article
    Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840
    Insert
    p21 Activated kinase 4 (PAK4 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Second predicted miR-199a-3p binding site mutated…
    Promoter
    PGK
    Available Since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    113093-plasmid-map-sequence-id-216990

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  16. pCIG1_UL136_26kDa_Myc

    Plasmid
    #74955
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 26kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    Strep II Tag (GGGGWSHPQFEKGGGGWSHPQFEK)
    Expression
    Mammalian
    Mutation
    delta (1-62) M78A M80A M100A M128A
    Promoter
    CMV, Lentiviral LTR
    Available Since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74955-plasmid-map-sequence-id-141778

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  17. pCIG1_UL136_33kda_Myc

    Plasmid
    #74957
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 33kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    Myc tag (EQKLISEEDL)
    Expression
    Mammalian
    Mutation
    M63A M78A M80A M100A M128A
    Promoter
    CMV, Lentiviral LTR
    Available Since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74957-plasmid-map-sequence-id-141779

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  26. LV-indLS2

    Plasmid
    #123068
    Purpose
    Lentiviral construct that delivers a tamoxifen inducible Cre. Upon recombination, luc2 and mStrawberry express. Used to image spontaneous tumorigenesis or subclonal cell populations in vivo.
    Depositor
    Kevin Brindle
    Article
    Rodriguez et al J Pathol. 2014 Mar;232(4):449-57. doi: 1
    Inserts
    CreERT2 with intron
    Firefly luciferase
    mStrawberry
    Use
    Lentiviral and Luciferase; Fluorescence
    Expression
    Mammalian
    Mutation
    synthetic intron added as indicated
    Promoter
    CAGGS (after Cre mediated inversion) and CAGGS (f…
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123068-plasmid-map-sequence-id-240523

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  29. pl-Synapsin-YFP-NaVII-III

    Plasmid
    #91246
    Purpose
    Expresses YFP-NaVII-III from the Synapsin promoter, for neuron-specific and activity-independent live label of the Axon Initial Segment (AIS)
    Depositor
    Matthew Grubb
    Article
    Dumitrescu et al Front Cell Neurosci. 2016 Nov 23;10:268.
    Insert
    Nav1.2 (Scn2a Rat)
    Use
    Lentiviral
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    The insert codes only for the intracellular loop …
    Available Since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    91246-plasmid-map-sequence-id-180624

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  35. LiOn*-CAG∞RFP

    Plasmid
    #154019
    Purpose
    Vector based on the LiOn integration-coupled translational switch and devoid of TTAA sequences, expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase
    Depositor
    Jean Livet
    Article
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    mRFP1
    Expression
    Mammalian
    Mutation
    All TTAA in vector backbone were replaced by sile…
    Promoter
    CAG
    Available Since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    154019-plasmid-map-sequence-id-308117

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  38. WT Smarca4-V5 IRES-puro

    Plasmid
    #107058
    Purpose
    Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.
    Depositor
    Jerry Crabtree, Courtney Hodges
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    IRES-puroR and V5
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CAG
    Available Since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107058-plasmid-map-sequence-id-201960

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  39. pA-CBh.TP-(inactive)CNOT6-V5H6.MCh.Puro

    Plasmid
    #209943
    Purpose
    In mammalian cells expresses TP fused to an inactive CNOT6 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.
    Depositor
    Kevin Weeks
    Article
    Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38
    Inserts
    CCR4-NOT transcription complex subunit 6 (enzymatically inactive) (CNOT6 Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    Tags
    TP (MS2 coat protein) and V5 epitope with H6 tag
    Expression
    Mammalian
    Promoter
    CBh and CMV
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    209943-plasmid-map-sequence-id-418717

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Showing: 8321 - 8360 of 9862 results