We narrowed to 17,995 results for: puro

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pLJM1-Halo-NLS

Plasmid

#250858

Purpose

lentiviral vector for expressing HaloTag protein with an SV40 NLS signal

Depositor

Britt Glaunsinger

Insert

HaloTag

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pLV V5-Mospd1

Plasmid

#175132

Purpose

Lentiviral expression of V5-tagged mouse Mospd1

Depositor

Larry Gerace

Insert

Mospd1 (Mospd1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Tyw1-V5

Plasmid

#175131

Purpose

Lentiviral expression of mouse Tyw1-V5

Depositor

Larry Gerace

Insert

Tyw1 (Tyw1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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StarShip01

Plasmid

#164505

Purpose

To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains T7-HaloTag-PDGFR_TM-T2A-Puro.

Depositor

Bo Huang

Type

Empty backbone

Use

Bxbi site specific integration

Availability

Academic Institutions and Nonprofits only

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StarShip02

Plasmid

#164506

Purpose

To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES(Myb)-T7-Halo-PDGFR_TM-T2A-Puro.

Depositor

Bo Huang

Type

Empty backbone

Use

Bxbi site specific integration

Availability

Academic Institutions and Nonprofits only

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StarShip03

Plasmid

#164507

Purpose

To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES2-T7-Halo-PDGFR_TM-T2A-Puro.

Depositor

Bo Huang

Type

Empty backbone

Use

Bxbi site specific integration

Availability

Academic Institutions and Nonprofits only

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PX459v3-eSpCas9(1.1)

Plasmid

#178800

Purpose

High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

Cbh (Cas9-2A Puro) and U6 (gRNA)

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pEN243-EN1953

Plasmid

#224546

Purpose

Cas9-2A-puro and sgRNA targeting the first coding ATG of mouse Nipbl

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

Cas9-2A-puro and sgRNA targeting the first coding ATG of mouse Nipbl (Nipbl Mouse)

Expression

Mammalian

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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CROP-seq-mTurquoise2-v2-no-scaffold

Plasmid

#228522

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using mTurquoise2 as selection marker

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Insert

mTurquoise2

Use

Lentiviral

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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CROP-seq-copGFP-v2-no-scaffold

Plasmid

#228520

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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PX459v3-SpCas9-HF1

Plasmid

#178801

Purpose

High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

Cbh (Cas9-2A Puro) and U6 (gRNA)

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-N3FLAG-TAF12(50-130)

Plasmid

#105595

Purpose

retrovirally express mouse TAF12 HFD with 3*FLAG tag at N terminal, GFP marker and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 (AA 50-130)- histone fold domain-HFD (Taf12 Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

truncation fragments containing aa (50-130), and …

Promoter

MSCV-LTR

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57.1

Plasmid

#41393

Purpose

3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro

Depositor

David Root

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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FU_tetO_dCas9-VP192-T2A-EGFP

Plasmid

#121173

Purpose

Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.

Depositor

Timo Otonkoski

Insert

dCas9-VP192-T2A-EGFP

Use

CRISPR and Lentiviral; Inducible by doxycycline

Tags

T2A-EGFP-ires-puro

Expression

Mammalian

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Hygro

Plasmid

#127763

Purpose

Used for expression of sgRNA in mammalian cells

Depositor

Ken-Ichi Takemaru

Type

Empty backbone

Expression

Mammalian

Available Since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Neo

Plasmid

#127762

Purpose

Used for expression of sgRNA in mammalian cells

Depositor

Ken-Ichi Takemaru

Type

Empty backbone

Expression

Mammalian

Available Since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLV Tmx1-V5

Plasmid

#175138

Purpose

Lentiviral expression of V5-tagged mouse Tmx1

Depositor

Larry Gerace

Insert

Tmx1 (Tmx1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLV V5-Nesprin-3

Plasmid

#175136

Purpose

Lentiviral expression of V5-tagged mouse Syne3

Depositor

Larry Gerace

Insert

Syne3 (Syne3 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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katushka-FMDV-P2A-pTRIP

Plasmid

#163522

Purpose

Used to construct lentivirus to express katushka in mammalian cells with option for a second insert

Depositor

Thomas Weber

Insert

stopless Katushka-P2A

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSB700-mouse-TTN-Cerulean

Plasmid

#128325

Purpose

SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)

Depositor

Alejandro Chavez

Insert

gRNA against mouse TTN for activation

Expression

Mammalian

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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