162,717 results

1. pDNR-gRNA

   Plasmid

   #206990

   Purpose

   A plasmid for expression of SaCas9-sgRNA targeting donor plasmids

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Insert

   Donor plasmid-targeting SaCas9-gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDNR-mCherry-T2A-3xPuroR-Frame 1

   Plasmid

   #206992

   Purpose

   Donor plasmid for Frame 1 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDNR-mCherry-T2A-3xPuroR-Frame 2

   Plasmid

   #206993

   Purpose

   Donor plasmid for Frame 2 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT3TS-spCas9-ABE-Umax-rest1

   Plasmid

   #222144

   Purpose

   Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-Kif2A

   Plasmid

   #52401

   Purpose

   Expresses GFP-tagged Kif2A in mammalian cells under the CMV promoter

   Depositor

   Ryota Uehara, Gohta Goshima

   Article

   Uehara et al J Cell Biol. 2013 Aug 19;202(4):623-36.

   Insert

   Kif2A (KIF2A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT3TS-spCas9 ABE-Umax-ex2-rest1

   Plasmid

   #222143

   Purpose

   Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1_nV5-AMOTL1

   Plasmid

   #172998

   Purpose

   constitutive expression of V5-tagged AMOTL1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   V5-AMOTL1 (AMOTL1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221-NPC1_STOP

   Plasmid

   #161461

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   NPC1 (NPC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCMV_SS-HA-V5-LaccID-CD4 TM_pDisplay

   Plasmid

   #234046

   Purpose

   expresses LaccID on the mammalian cell surface

   Depositor

   Alice Ting

   Article

   Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

   Insert

   LaccID-CD4TM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PE6g

    Plasmid

    #207857

    Purpose

    Mammalian expression of PE6g prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6g
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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