We narrowed to 2,512 results for: organization Addgene

1. pEF-pIgH-ESCargo(FTV)

   Plasmid

   #140160

   Purpose

   Expresses a regulatable secretory cargo for mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. piggybac-EF1-H2AZ-halo

   Plasmid

   #197907

   Purpose

   single-particle tracking for H2AZ

   Depositor

   James Zhe Liu

   Article

   Dong et al bioRxiv 2022.10.26.513917

   Insert

   H2az2 (H2az2 Mouse)
   

   Use

   Tags

   Halotag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSecTag-ATX

   Plasmid

   #17839

   Depositor

   Steven Rosen

   Article

   Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

   Insert

   Autotaxin (ENPP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1(-)-EGFP-FP4-mito

   Plasmid

   #229692

   Purpose

   Transient expression of EGFP FP4-mito in mammalian cells

   Depositor

   Alpha Yap

   Article

   Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

   Insert

   EGFP; Mitochondira FP4 motifs from Listeria ActA
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1(-)-EGFP-AP4-mito

   Plasmid

   #229693

   Purpose

   Transient expression of EGFP AP4-mito in mammalian cells

   Depositor

   Alpha Yap

   Article

   Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

   Insert

   EGFP; Control construct with tandem AP4 motifs (mitochondria)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5-FRT/TO-Flag-RNF169

   Plasmid

   #74243

   Purpose

   Expresses RNF169 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Panier et al Mol Cell. 2012 Aug 10;47(3):383-95. doi:

   Insert

   RNF169 (RNF169 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pEGFP-C1-hFGD5

   Plasmid

   #50760

   Purpose

   expression of EGFP-tagged human FGD5

   Depositor

   Naoki Mochizuki

   Article

   Ando et al J Cell Biol. 2013 Sep 16;202(6):901-16.

   Insert

   FGD5 (FGD5 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEF-pIgH-ESCargo(FTV,Crimson)

   Plasmid

   #140162

   Purpose

   Expresses a regulatable secretory cargo for mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   piGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
   

   Use

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-His-MinD_MinE

   Plasmid

   #133621

   Purpose

   Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

   Depositor

   Petra Schwille

   Article

   Loose et al Science. 2008 May 9;320(5877):789-92. do

   Insert

   His-MinD_MinE (minD, minE E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCSIIbsr-4082NES(ForAMPK)

    Plasmid

    #170310

    Purpose

    The lentiviral vector for AMPK FRET-biosensor

    Depositor

    Michiyuki Matsuda

    Article

    Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. d

    Insert

    4082NES (FRET biosensor for AMPK activity)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mCherry-miniSOG-C1

    Plasmid

    #54801

    Purpose

    Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Type

    Empty backbone

    Use

    Tags

    mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA5/FRT/TO APEX2-eIF4E1

    Plasmid

    #129644

    Purpose

    Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells

    Depositor

    Nicholas Ingolia

    Article

    Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

    Insert

    EIF4E1 (EIF4E Human)
    

    Use

    Tags

    APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV6M-PAK1 H83L H86L K299R

    Plasmid

    #26592

    Depositor

    Jonathan Chernoff

    Article

    Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    H83L H86L K299R Dominant negative

    Promoter

    Available since

    March 9, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pEF-pIgH-ESCargo*

    Plasmid

    #140159

    Purpose

    Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. px459 sgAtg5

    Plasmid

    #175023

    Purpose

    CRISPR-Cas9 plasmid targeting exon 2 of human Atg5.

    Depositor

    David Tumbarello

    Article

    Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

    Insert

    ATG5 (ATG5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSecTag-T210A-ATX

    Plasmid

    #17840

    Depositor

    Steven Rosen

    Article

    Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

    Insert

    T210A-ATX (ENPP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

17. miniSOG-RatLC3

    Plasmid

    #57775

    Purpose

    Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    LC3
    

    Use

    Tags

    miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pEF-pIgH-ESCargo(FTV,Orange)

    Plasmid

    #140161

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUBC_V5_MS2-mVenus

    Plasmid

    #107606

    Purpose

    Transient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tag

    Depositor

    John Rinn

    Article

    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

    Insert

    MS2-mVenus
    

    Use

    Synthetic Biology

    Tags

    V5 and mVenus

    Expression

    Mutation

    MS2: V75E/A81G

    Promoter

    human UBC

    Available since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCherry-miniSOG-N1

    Plasmid

    #54803

    Purpose

    Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Type

    Empty backbone

    Use

    Tags

    mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only