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Plasmid#26386DepositorTypeEmpty backboneUseTagsExpressionBacterial and WormMutationPromoterAvailable sinceOct. 29, 2010AvailabilityAcademic Institutions and Nonprofits only
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pSiM4
Plasmid#199383PurposeEncodes dual cistronic membrane localized jGCaMP7f and mScarlet-I optimized for C. elegans expressionDepositorInsertjGCaMP6f::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
UseTagsExpressionBacterialMutationno mutations, except added subcellular localizati…PromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS1-rab-3P::his-24::tagBFP
Plasmid#124343PurposeNuclear Localized Pan-Neuronal tagBFPDepositorInsertrab-3, tagBFP (rab-3 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIN4_tsp-6_mScarlet
Plasmid#182935PurposeExpresses tsp-6::mScarlet under its native promoter (amphid and phasmid ciliated neurons in C. elegans)DepositorInsertPtsp-6-3::tsp-6::flexible linker::mScarlet (C. elegans, genomic)
UseTagsflexible linker (GGGGS)x3 and mScarlet tagExpressionWormMutationPromotertsp-6Available sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pJH2685
Plasmid#191372PurposePunc-4-TeTx::mCherry unc-54 3' UTR C.elegans A MNs/others neurou expression of TeTx RFPDepositorInsertTetanus Toxin Light Chain
UseTagswCherryExpressionWormMutationPromoterPunc-4Available sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
SP6-hCas9-Ce-mRNA
Plasmid#47911PurposeIn vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.DepositorInserthCas9 with C. elegans 5' and 3' UTRs
UseCRISPRTagsExpressionMutationPromoterSP6Available sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-17b_Unc104-KIF5B-HALO
Plasmid#169996PurposeUltra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labelingDepositorInsertKinesin-like protein Unc-104 fused with KIF5B
UseTagsHALO-tag, 6xHISExpressionBacterialMutationPromoterT7Available sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS051
Plasmid#137025PurposeSapTrap mScarlet-I-GLO donorDepositorInsertC. elegans GLO-optimized mScarlet
UseCRISPR; Saptrap donor for cloning repair templat…TagsC. elegans GLO-optimized mScarletExpressionWormMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
punc-119cR
Plasmid#21596DepositorInsertunc-119:mCherry (unc-119 Nematode)
UseAdds unc-119 marker to amp resistant plasmidsTagsmCherryExpressionWormMutationN/APromoterAvailable sinceAug. 26, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSEM253 - sgRNA mosTI hygroR
Plasmid#159827PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertsgRNA mosTI hygroR
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1-odr-4P::his-24::mNeptune
Plasmid#124342PurposeMulticolor Worms for Neuronal IDDepositorInsertodr-4, mNeptune (odr-4 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pMLS328
Plasmid#73717PurposeC. elegans germline CRE expression vectorDepositorInsert2xNLS-CRE
UseCre/LoxTagsExpressionWormMutationPromoterPeft-3Available sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
SNF-5 pXOOM
Plasmid#48697PurposeExpressed SNF-5 in Xenopus oocytes or mammalian cellsDepositorInsertsnf-5 (snf-5 Nematode)
UseXenopus oocyte expressionTagsExpressionMammalianMutationK42E (when compared to NP_496735.1)PromoterT7Available sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7)
Plasmid#164040PurposeExpression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2DepositorInsertsgTS5-sgTS6-sgTS7
UseCRISPRTagsExpressionMutationPromotermouse U6Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJW6.01
Plasmid#62882PurposeTransgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTRDepositorInsertGFP::MEG-3
UseTagsGFPExpressionWormMutationPromotermeg-3Available sinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMLS791
Plasmid#188889PurposeTargeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFPDepositorInsertfloxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
UseTagsExpressionWormMutationC. elegans codom optimization and intron additionPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW2088
Plasmid#154336PurposeCrispr repair template: add insertion site specific homology arms by PCR before insertionDepositorInsert30aa linker::GFP::3xFLAG::10aa linker
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2359 - intron GG2 - 250bp
Plasmid#159878PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertintron GG2 - 250bp
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only