Addgene

We narrowed to 1,532 results for: Tef;

Showing: 601 - 630 of 1532 results

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-2

Plasmid

#128763

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #2 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1

Plasmid

#128762

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #1 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-shLARP7

Plasmid

#113537

Purpose

Retroviral vector designed to knock down LARP7.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shLARP7 (LARP7 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1-MePCE_aa470-689

Plasmid

#113544

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 470 - 689.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1_MePCE_aa432-689

Plasmid

#113543

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 432 - 689.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1_MePCE_aa400-689

Plasmid

#113542

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 400 - 689.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1_MePCE_aa1-400

Plasmid

#113541

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 1 - 400.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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SP65U1-135a-1

Plasmid

#105819

Purpose

plasmid for the overexpression of miR-135a under the U1 expression cassette inserted in the pSP65 plasmid

Depositor

Cecilia Mannironi

Article

Mannironi et al PLoS One. 2013 Sep 4;8(9):e73385. doi: 1

Insert

MIRNA135A1 (Mir135a-1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U1

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1-LARP7

Plasmid

#113545

Purpose

Produces an N-terminal GST-tagged full length LARP7 protein.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

LARP7 (LARP7 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Abi1

Plasmid

#74905

Purpose

Expresses human Abi1 fused to GFP

Depositor

Giorgio Scita

Article

Innocenti et al Nat Cell Biol. 2004 Apr;6(4):319-27. Epu

Insert

Abi1 (ABL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pEF myc Gal cyclin T1 (1-726) (P#1026)

Plasmid

#14626

Depositor

Matija Peterlin

Article

Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

Insert

cyclin T1 (CCNT1 Human)

Use

Tags

Gal and myc

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pBABE-Flag-Cdk9-IRES-eGFP

Plasmid

#28096

Depositor

Andrew Rice

Article

Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

Insert

Cdk9 (CDK9 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
p6345 MSCV-CMV-Flag-HA-Brd4 1-722

Plasmid

#31352

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 1-722 (BRD4 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

*see comments

Promoter

Available since

Oct. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pG2J

Plasmid

#102882

Purpose

Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Inserts

OsTIR1
mGFP-AID

Use

Tags

AID(71-114)

Expression

Yeast

Mutation

Promoter

ADH1 and TEF1

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-Intron-CterFlag-MePCE

Plasmid

#113549

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

Plasmid

#89873

Purpose

expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

bArrestin2-TevC-P2A-TdTomato

Use

AAV

Tags

TdTomato

Expression

Mammalian

Mutation

R173H in TdTomato (Please see depositor comments …

Promoter

Human synapsin

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYN-DRD2-V2tail-TevN-BLITz1-TetR-VP16-bGHpA

Plasmid

#89874

Purpose

expresses DRD2-iTango component in mammalian cells for AAV production

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

DRD2-V2tail-TevN-BLITz1-TetR-VP16

Use

AAV

Tags

FLAG

Expression

Mammalian

Mutation

short isoform of DRD2 (deletion of 242-270)

Promoter

Human synapsin

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-Flag-Cdk9-D167N-IRES-eGFP

Plasmid

#28098

Depositor

Andrew Rice

Article

Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

Insert

Cdk9 (CDK9 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Contains D167N mutation in Cdk9

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
pBABE-Flag-Cdk9-T186A-IRES-eGFP

Plasmid

#28097

Depositor

Andrew Rice

Article

Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

Insert

Cdk9 (CDK9 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Contains T186A mutation in Cdk9

Promoter

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
p6348 MSCV-CMV-Flag-HA-Brd4 1047-1362

Plasmid

#31354

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 1047-1362 (BRD4 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
p6347 MSCV-CMV-Flag-HA-Brd4-444-722

Plasmid

#31353

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 444-722 (BRD4 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
p6349 MSCV-CMV-Flag-HA-Brd4 1224-1362

Plasmid

#31355

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 1224-1362 (BRD4 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF myc Gal CDK9 (P#1475)

Plasmid

#14631

Depositor

Matija Peterlin

Article

Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

Insert

cdk9 (CDK9 Human)

Use

Tags

Gal and myc

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pIRGAL43C

Plasmid

#218278

Purpose

Introduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter induction

Depositor

Claudia Vickers

Article

Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

Insert

fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)

Use

Tags

Expression

Yeast

Mutation

GAL3 (S509P)

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRGAL4A

Plasmid

#218277

Purpose

Introduction of a LowTempGAL Turbo module (GAL4) to accelerate induction of GAL promoter induction

Depositor

Claudia Vickers

Article

Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

Insert

fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-fcy1(454-778)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRGAL3C

Plasmid

#218275

Purpose

Introduction of a LowTempGAL Turbo module (GAL3C) to accelerate induction of GAL promoter induction

Depositor

Claudia Vickers

Article

Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

Insert

fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)

Use

Tags

Expression

Yeast

Mutation

GAL3 (S509P)

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW57.1_BN006A-P_V5_FLAG_C

Plasmid

#218062

Purpose

RESOLUTE SLC binder lentiviral plasmid

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN006A-P

Use

Lentiviral

Tags

V5-FLAG

Expression

Mutation

Promoter

Available since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW57.1_BN001M-T_V5_FLAG_C

Plasmid

#218063

Purpose

RESOLUTE SLC binder lentiviral plasmid

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN001M-T

Use

Lentiviral

Tags

V5-FLAG

Expression

Mutation

Promoter

Available since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW57.1_BN006A-P_V5_mNG_FLAG_C

Plasmid

#218076

Purpose

RESOLUTE SLC binder lentiviral plasmid

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN006A-P

Use

Lentiviral

Tags

FLAG-mNeonGreen-V5

Expression

Mutation

Promoter

Available since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXNPFLAGH_BN001M-T

Plasmid

#218078

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN001M-T

Use

Tags

FLAG-His and PelB

Expression

Bacterial

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge