1,878 results

1. pJA50

   Plasmid

   #59931

   Purpose

   gRNA for cleavage at unc-58(e665) locus in C elegans

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Insert

   unc-58(e665) gRNA
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSAH61 - [ASELp | wrmScarlet | tbb-2 UTR]

   Plasmid

   #200335

   Purpose

   mScarlet BioPart for ASEL neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.

   Depositor

   Christian Froekjaer-Jensen

   Insert

   [ASELp | wrmScarlet | tbb-2 UTR]
   

   Expression

   Worm

   Promoter

   WBGene00001534, gcy-7

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCM3.19

   Plasmid

   #19401

   Depositor

   Geraldine Seydoux

   Article

   Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

   Insert

   tbb-2 ORF+3' in pDONR201 (tbb-2 Nematode)
   

   Expression

   Worm

   Available Since

   Nov. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCFJ2300 - [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)

   Plasmid

   #159869

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. BSP608

   Plasmid

   #122253

   Purpose

   expression vector for mNeptune under EFT3 promoter

   Depositor

   Alexander Mendenhall

   Article

   Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

   Insert

   mNeptune
   

   Expression

   Worm

   Promoter

   EFT3

   Available Since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. L4440-daf-16df

   Plasmid

   #31505

   Depositor

   Heidi Tissenbaum

   Article

   Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

   Insert

   daf-16d/f specific (daf-16 Nematode)
   

   Use

   RNAi

   Available Since

   Nov. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pXF85

   Plasmid

   #139051

   Purpose

   MosSCI SapTrap assembly: Cassette 4 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   pie-1 3'UTR (pie-1 Nematode)
   

   Expression

   Worm

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBN278

   Plasmid

   #86724

   Purpose

   Tissue-specific FLP expression plasmid for C. elegans

   Depositor

   Peter Askjaer

   Article

   Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

   Insert

   Pmec-7::FLP D5
   

   Expression

   Worm

   Available Since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSEM228 - [Pmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR]

   Plasmid

   #159895

   Purpose

   Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

   Insert

   Pmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR
   

   Expression

   Worm

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. L4440-daf-16abf

    Plasmid

    #31507

    Depositor

    Heidi Tissenbaum

    Article

    Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

    Insert

    daf-16a/b/f common (daf-16 Nematode)
    

    Use

    RNAi

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pSA108

    Plasmid

    #59786

    Purpose

    drives mCherry in C. elegans ubiquitously

    Depositor

    Jeremy Nance

    Article

    Armenti et al Development. 2014 Dec;141(23):4640-7. do

    Inserts

    cdc-42 promoter
    mCherry (worm)
    unc-54 3' UTR
    

    Expression

    Bacterial

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCC336

    Plasmid

    #110167

    Purpose

    miniMos vector expresses ERKKTR(S43E,T55E,T62E)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)

    Depositor

    Iva Greenwald

    Article

    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

    Insert

    ERK-KTR(S43E,T55E,T62E)-mClover-T2A-mCherry-H2B
    

    Use

    Minimos transposon

    Tags

    mCherry and mClover

    Expression

    Worm

    Mutation

    Phospho-acceptor sites mutated: S43E, T55E, T62E.…

    Promoter

    lin-31 promoter/enhancer

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCC335

    Plasmid

    #110166

    Purpose

    miniMos vector expresses ERKKTR(S43A,T55A,T62A)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)

    Depositor

    Iva Greenwald

    Article

    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

    Insert

    ERK-KTR(S43A,T55A,T62A)-mClover-T2A-mCherry-H2B
    

    Use

    Minimos transposon

    Tags

    mCherry and mClover

    Expression

    Worm

    Mutation

    Phospho-acceptor sites mutated: S43A, T55A, T62A.…

    Promoter

    lin-31 promoter/enhancer

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pID3.01B

    Plasmid

    #22720

    Depositor

    Geraldine Seydoux

    Article

    D'Agostino et al Dev Biol. 2006 Apr 1. 292(1):244-52.

    Insert

    unc-119 rescuing fragment / pie-1promoter-GFP- gateway destination cassette B -pie-1 3’
    

    Expression

    Bacterial

    Available Since

    Jan. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ploxP unc-119

    Plasmid

    #19185

    Depositor

    Al Fisher

    Article

    Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

    Insert

    unc-119 (unc-119 Nematode)
    

    Use

    Recombineering

    Mutation

    contains loxP site just 5' to ApaI site

    Available Since

    Jan. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCFJ2259 - Intron GG2 - 900 bp

    Plasmid

    #159881

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG2 - 900 bp
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCFJ1001

    Plasmid

    #44476

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, unc-119, peel-1, [4-3] Gateway
    

    Use

    Gateway destination vector

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSEM236 - pEXP[Pmlc-2 | histamine | rpl-3 UTR]

    Plasmid

    #159797

    Purpose

    Histamine based negative selection marker to paralyze animals with arrays

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

    Insert

    pEXP[Pmlc-2 | histamine | rpl-3 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCC20

    Plasmid

    #198783

    Purpose

    Drives specific expression in the ASK neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    Psra-9-GAL4-SK(DBD)-VP64
    

    Expression

    Worm

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJW1883

    Plasmid

    #154331

    Purpose

    sgRNA Target Vector for CRISPR

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    ttTi5605 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNM3749

    Plasmid

    #154108

    Purpose

    loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Inserts

    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::his-58::FRT3
    

    Use

    Cre/Lox; Rmce

    Expression

    Worm

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCFJ496

    Plasmid

    #44488

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    500bp Mos1, unc-119, fosmid cassette
    

    Use

    Fosmid recombineering cassette

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCFJ2441 - [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159870

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAP997-1

    Plasmid

    #99500

    Purpose

    3XFlag::tagRFP::linker::TEV

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    3XFlag::tagRFP::linker::TEV
    

    Expression

    Bacterial

    Available Since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBN303

    Plasmid

    #86730

    Purpose

    Tissue-specific FLP expression plasmid for C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    Punc-47::FLP D5
    

    Expression

    Worm

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJW1947

    Plasmid

    #154339

    Purpose

    Enhancer reporter

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    pes-10delta::SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR
    

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSA107

    Plasmid

    #59785

    Purpose

    drives ZIF-1 protein in C. elegans ubiquitously

    Depositor

    Jeremy Nance

    Article

    Armenti et al Development. 2014 Dec;141(23):4640-7. do

    Inserts

    cdc-42 promoter (NEWENTRY Nematode)
    zif-1 coding sequence (zif-1 )
    unc-54 3' UTR
    

    Expression

    Bacterial

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBN260

    Plasmid

    #86722

    Purpose

    Tissue-specific FLP expression plasmid for C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    Pmyo-3::FLP D5
    

    Expression

    Worm

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJW1241

    Plasmid

    #74904

    Purpose

    Expresses 3XFLAG::GR LBD::eGFP from the hsp16.48 promoter

    Depositor

    Jordan Ward, Keith Yamamoto

    Article

    Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

    Insert

    3X FLAG::GR LBD::eGFP (NR3C1 Human, Synthetic, Nematode)
    

    Expression

    Worm

    Promoter

    hsp16.48

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCFJ1002

    Plasmid

    #44477

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, NeoR, peel-1, [4-3] Gateway
    

    Use

    Gateway destination vector

    Available Since

    May 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge