Addgene

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pRSFDuet-1_StefinA

Plasmid

#182402

Purpose

Bacterial expression of stefinA

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

stefin (CSTA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_hGH

Plasmid

#182410

Purpose

Bacterial expression of human growth hormone

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

hGH (GH1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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p6874 pHAGE-N-V5-MAML1-ADDAA

Plasmid

#37050

Depositor

Peter Howley

Article

Tan et al Proc Natl Acad Sci U S A. 2012 Apr 30.

Insert

MAML1-ADDAA (MAML1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMVt

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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Dll1ICD-HPC4 pBacPAK8-3

Plasmid

#17333

Depositor

Ronald Conaway, Joan Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Delta-like 1 (Dll1 Mouse)

Use

Tags

HPC4

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pRSFDuet-1_VNp-DARPinOFF7

Plasmid

#182397

Purpose

Bacterial expression of Vesicle Nucleating peptide-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-DARPinOFF7

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Jag2ICD-HA pBacPAK8-3

Plasmid

#17332

Depositor

Ronald Conaway, Joan Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Jagged2 (Jag2 Rat)

Use

Tags

HA

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
Jag1ICD-HA pBacPAK8-3

Plasmid

#17331

Depositor

Ronald Conaway, Joan Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Jagged1 (Jag1 Rat)

Use

Tags

HA

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
Dll3ICD-HPC4 pBacPAK8-3

Plasmid

#17334

Depositor

Ronald Conaway, Joan Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Delta-like 3 (Dll3 Mouse)

Use

Tags

HPC4

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
Bambi NGFR

Plasmid

#44628

Depositor

Ellen Rothenberg

Article

Del Real et al Development. 2013 Mar;140(6):1207-19. do

Insert

Bambi (Bambi Mouse)

Use

Retroviral

Tags

IRES and truncated NGFR

Expression

Mammalian

Mutation

Promoter

Available since

April 17, 2013

Availability

Academic Institutions and Nonprofits only
Neurl1-FLAG/Jag1-HA pIRES

Plasmid

#17337

Depositor

Ronald Conaway, Joan Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Neuralized-like (Neurl1a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
Neurl1G2A-Flag pcDNA3.1

Plasmid

#17320

Depositor

Ronald Conaway, Joan Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Neuralized-like (Neurl1a Mouse)

Use

RNAi

Tags

FLAG

Expression

Mammalian

Mutation

Gly 2 changed to Ala

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pRSFDuet-1_FGF21

Plasmid

#182406

Purpose

Bacterial expression of fgf21

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

fgf21 (FGF21 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-LZ_CydAB-mNeongreen

Plasmid

#182393

Purpose

Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and cydAB--mNeongreen fusions

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Inserts

VNp-LZ
CydAB-mNeongreen

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pNCMV HPV8 E6 del132-136

Plasmid

#87658

Purpose

Flag/HA tagged HPV8 E6 del132-136

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

HPV 8E6

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

Deletion of aa132-136

Promoter

CMV

Available since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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Neurl1Rm-Flag pBacPAK8-3

Plasmid

#17326

Depositor

Ronald Conaway, Joan Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Neuralized-like (Neurl1a Mouse)

Use

Tags

FLAG

Expression

Insect

Mutation

Cys 521 changed to Ser Cys 524 changed to Ser

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pRSFDuet-1_mNeongreen-Etanercept

Plasmid

#182420

Purpose

Bacterial expression of mNeongreen-Etanercept fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-Etanercept (TNFRSF1B Human)

Use

Tags

mNeongreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR-Cre stuffer v4

Plasmid

#158033

Purpose

lenti-viral construct with Cas9, Cre recombinase and U6 driven sgRNA casette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pmROSA26-attP50-Neo-mKate2-3xNLS-attP50

Plasmid

#183609

Purpose

Targeting vector for mouse ROSA26 locus to create landing pad cell line. Introduces attP50 sites flanking a Neo gene driven by endogenous locus (confers G418 resistance) and a CAG-mKate2-3xNLS.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Inserts

Neo
mKate2-3xNLS

Use

Mouse Targeting

Tags

3xNLS

Expression

Mutation

Promoter

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-Cre sgAmotl2 v3

Plasmid

#158602

Purpose

lenti-viral construct with Cas9, Cre recombinbase and U6 driven sgRNA against mouse Amotl2

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Amotl2 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-CreERT2 stuffer v3

Plasmid

#158046

Purpose

lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge