We narrowed to 13,477 results for: crispr cas9

1. pEasyCas9

   Plasmid

   #184909

   Purpose

   Expresses the Streptococcus pyogenes Cas9 protein

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. eSpCas9-EF

   Plasmid

   #177267

   Purpose

   CRISPR vector

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDe_Cas9_KANA_R160a

   Plasmid

   #173756

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160a

   Depositor

   Kristina Gruden

   Article

   Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

   Insert

   Cas9_sgRNA1/R160a_sgRNA2/R160a
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   AtU6 &StU6

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDe_Cas9_KANA_R160b

   Plasmid

   #173757

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160b

   Depositor

   Kristina Gruden

   Article

   Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

   Insert

   Cas9_sgRNA1/R160b_sgRNA2/160b
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   AtU6 &StU6

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDe_Cas9_KANA_R390a

   Plasmid

   #173758

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA390a

   Depositor

   Kristina Gruden

   Article

   Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

   Insert

   Cas9_sgRNA1/390a_sgRNA2/390a
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   AtU6 &StU6

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. EC2_2_dCas9_VP64_sgRNA

   Plasmid

   #163707

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   VP64+SV40 NLS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pM-Cas9

   Plasmid

   #196325

   Purpose

   35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV M antigenome encoding SpCas9 in place of viral GP
   

   Use

   CRISPR

   Tags

   3×flag

   Expression

   Plant

   Mutation

   See depositor comments below

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Sniper-Cas9

   Plasmid

   #138559

   Purpose

   Expresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   Sniper-Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   F539S, M763I, K890N

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW-Cas9-2A-EGFP

   Plasmid

   #167928

   Purpose

   Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Insert

   T2A-EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tTRE, hPGK

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-TO-dCas9-GFP

    Plasmid

    #64106

    Purpose

    Expression of Sp dCas9-GFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCVpuro-Cas9-EGFP

    Plasmid

    #159126

    Purpose

    Expresses active Cas9 fused with EGFP for fluorescence microscopy

    Depositor

    Taekjip Ha

    Article

    Liu et al Science. 2020 Jun 12;368(6496):1265-1269

    Insert

    Cas9 fused to EGFP
    

    Use

    CRISPR and Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR promoter

    Available Since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-TO-dCas9-3XmCherry

    Plasmid

    #64108

    Purpose

    Expression of Sp dCas9-3XmCherry in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRS-XylS-dCas9

    Plasmid

    #220196

    Purpose

    mini-Tn7 dCas9 expression vector - XylS/pM promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    XylS/pM

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRS-LacI-dCas9

    Plasmid

    #220194

    Purpose

    mini-Tn7 dCas9 expression vector - LacI/pLac promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    LacI/pLac

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-SMVP-Cas9C

    Plasmid

    #80931

    Purpose

    Expresses Cas9C in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-TO-nmdCas9-3XmCherry

    Plasmid

    #64110

    Purpose

    Expression of Nm dCas9-3XmCherry in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Nm dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-TO-nmdCas9-3XGFP

    Plasmid

    #64109

    Purpose

    Expression of Nm dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Nm dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113343

    Purpose

    Expresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    p300core-dCas9-VP64-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas9–mKate2ps–CgRNA

    Plasmid

    #64955

    Purpose

    Control gRNA (GTCAAGGCACTCTTGCCTA)

    Depositor

    Leonidas Bleris

    Article

    Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

    Insert

    Cas9–mKate2ps
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCas9–mKate2ps–T1gRNA

    Plasmid

    #62717

    Purpose

    t1 gRNA (ATGAGAATCAAGGCGGTCGA)

    Depositor

    Leonidas Bleris

    Article

    Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

    Insert

    Cas9–mKate2ps
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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