We narrowed to 29,388 results for: des.2

1. pKDR-EF1a.2

   Plasmid

   #183569

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 2
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. SI4-Jamc.2

   Plasmid

   #28216

   Depositor

   Gavin Wright

   Article

   Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

   Insert

   Jamc.2 mouse monoclonal antibody (jam3a Zebrafish)
   

   Expression

   Mammalian

   Available Since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRG764-SoxB2-2

   Plasmid

   #99088

   Purpose

   dsRNA and riboprobe synthesis for Schmidtea mediterranea SoxB2-2

   Depositor

   Phillip Newmark

   Article

   Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

   Insert

   SoxB2-2 in situ probe
   

   Use

   T/a cloning vector for dsrna generation and the g…

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pconst #2-RFP

   Plasmid

   #81076

   Purpose

   constitutive promoter expressing RFP, biobrick construction

   Depositor

   Jay Keasling, Paul Adams

   Article

   Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

   Insert

   RFP
   

   Use

   P15a origin, constitutive promoter, kanr

   Promoter

   Constitutive Promoter- BBa_J23104

   Available Since

   Aug. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-p107 2*

   Plasmid

   #32388

   Depositor

   Julien Sage

   Article

   Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

   Insert

   p107 promoter (Rbl1 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   2nd E2F binding site mutated: GTC-->AAA

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

6. psiT-GEM(2)

   Plasmid

   #220448

   Purpose

   Split intein version of Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

   Depositor

   Benjamin White

   Article

   Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

   Insert

   CfaN-Gal4-CfaC
   

   Expression

   Insect

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXPR_003_sgTFAP4 guide 2

   Plasmid

   #193587

   Purpose

   TFAP4 knockout

   Depositor

   William Hahn

   Article

   Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

   Insert

   sgTFAP4 guide 2 (TFAP4 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330A-T#2

   Plasmid

   #171516

   Purpose

   deletion of a genomic locus in T(Brachyury) gene

   Depositor

   Hans Schöler

   Article

   Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

   Insert

   T (T Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Bax gRNA#2

   Plasmid

   #171828

   Purpose

   Cas9-mediated knockout of Bax in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bax (Bax Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiCRISPRv2bleo-Nectin-2

    Plasmid

    #170295

    Purpose

    A knock-out vector for the mouse Nectin-2

    Depositor

    Michiyuki Matsuda

    Article

    Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

    Insert

    A gRNA targeting the mouse Nectin-2 gene.
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJIT163-AFID-2

    Plasmid

    #167358

    Purpose

    For inducing predictable deletions in protoplasts and wheat plants

    Depositor

    Caixia Gao

    Article

    Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

    Insert

    A3A-Cas9-UDG
    

    Expression

    Plant

    Mutation

    WT

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_OTOR-1/2

    Plasmid

    #91328

    Purpose

    Protein expression and purification of human SH3 domain construct OTOR-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    OTOR-1/2
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRRG170-LDLRR-2

    Plasmid

    #99094

    Purpose

    dsRNA and riboprobe synthesis for Schmidtea mediterranea LDLRR-2

    Depositor

    Phillip Newmark

    Article

    Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

    Insert

    LDLRR-2 in situ probe
    

    Use

    T/a cloning vector for dsrna generation and the g…

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-(RTRG)2

    Plasmid

    #69846

    Purpose

    The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

    Depositor

    Daniel Christophe

    Article

    Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

    Insert

    2 RTRG repeats
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKD237-3a-2

    Plasmid

    #90957

    Purpose

    Expresses ThsR and mCherry constitutively and sfGFP under the PphsA promoter

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Inserts

    Thiosulfate response regulator
    Superfolder GFP
    mCherry
    

    Expression

    Bacterial

    Promoter

    Bba_J23100, Bba_J23105, and PphsA

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKD239-1g-2

    Plasmid

    #90959

    Purpose

    Expresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoter

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Inserts

    Tetrathionate response regulator
    Superfolder GFP
    mCherry
    

    Expression

    Bacterial

    Promoter

    Bba_J23100, Bba_J23109, and PttrB185-269

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. IKK-2 WT

    Plasmid

    #11103

    Depositor

    Anjana Rao

    Article

    Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

    Insert

    IKK-2 (IKBKB Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. LVDP 2P.2

    Plasmid

    #231904

    Purpose

    For the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging firefly luciferase mRNA into the VLPs

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

    Insert

    nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, E, M SARS-CoV-2)
    

    Use

    Lentiviral

    Mutation

    R203M in N protein

    Promoter

    CMV, EF-1a

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV5 mSynCAM 2

    Plasmid

    #115497

    Purpose

    expression of mouse full-length SynCAM 2

    Depositor

    Thomas Biederer

    Article

    Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

    Insert

    Cadm2 (Cadm2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. YFP Gamma 2

    Plasmid

    #36102

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 2 (GNG2 Bovine)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only