We narrowed to 1,017 results for: hug;

1. pR-mKate2

   Plasmid

   #228610

   Purpose

   Mammalian expression of mKate2, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

   Insert

   mKate2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CIB81-pmGFP

   Plasmid

   #75366

   Purpose

   Expresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on end

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

   Insert

   CIB1 (CIB1 Mustard Weed)
   

   Use

   Tags

   prenylated EGFP

   Expression

   Mammalian

   Mutation

   residues 1-81 of CIB1

   Promoter

   CMV

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-ExRai-AktAR

   Plasmid

   #118462

   Purpose

   cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AktAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-sapphireCKAR(T/A)

   Plasmid

   #118469

   Purpose

   Negative-control mutant for sapphireCKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireCKAR(T/A)
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1(+)-blueAKAR(T/A)

   Plasmid

   #118471

   Purpose

   Negative-control mutant for blueAKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   blueAKAR(T/A)
   

   Use

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3 Paf1

   Plasmid

   #11059

   Depositor

   Matthew Meyerson

   Article

   Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

   Insert

   Paf1 (PAF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMXs-RAP2A-GFP

   Plasmid

   #86124

   Purpose

   Retroviral vector expressing RAP2A-GFP-IRES-Bsd

   Depositor

   David Sabatini

   Article

   Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Retroviral

   Tags

   3xFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CIB1-CreC(N1)

   Plasmid

   #75367

   Purpose

   Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinase

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

   Insert

   CIB1 (CIB1 Mustard Weed)
   

   Use

   Tags

   Cre DNA recombinase, residues 106-343

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pREDIT_MCP-RecT

   Plasmid

   #164803

   Purpose

   mammalian-optimized expression of SSAP RecT fused to MCP (for MS2 aptamer), pLenti-EF1A-MCP-EXTEN-RecT-NLS

   Depositor

   Le Cong

   Article

   Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

   Insert

   RecT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pR-CyOFP1

    Plasmid

    #228604

    Purpose

    Mammalian expression of CyOFP1, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    CyOFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCIB1(deltaNLS)-pmGFP

    Plasmid

    #28240

    Purpose

    Expresses CIB1 fused to plasma membrane-localized EGFP

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CIB1(deltaNLS)-pmGFP (CIB1 Mustard Weed)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    NLS sequences mutated

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEF5/FRT-DEST EVC2-Flag

    Plasmid

    #41005

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pGal4BD-CRY2PHR

    Plasmid

    #28244

    Purpose

    Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    cry2 (CRY2 Mustard Weed)
    

    Use

    Tags

    Gal4BD

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJS646

    Plasmid

    #183175

    Purpose

    Y2H vector compatible with pJS647 PYR1 insert

    Depositor

    Timothy Whitehead

    Article

    Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXs-NDUFAF5-HA

    Plasmid

    #86125

    Purpose

    Retroviral vector expressing NDUFAF5-HA-IRES-Bsd

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    NDUFAF5 (NDUFAF5 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-blueCKAR

    Plasmid

    #118472

    Purpose

    cpBFP-based single-color biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueCKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENTR-PKAKTRClover

    Plasmid

    #59146

    Purpose

    ORF encoding PKA KTR mClover flanked by gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    PKA Kinase Translocation Reporter (PRKACA Mouse, Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

    Plasmid

    #70658

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pREDIT_Cas9-MS2-BB_BbsI

    Plasmid

    #164802

    Purpose

    REDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFP

    Depositor

    Le Cong

    Article

    Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pREDIT_Cas9n-MS2-BB_BbsI

    Plasmid

    #164804

    Purpose

    REDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFP

    Depositor

    Le Cong

    Article

    Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge