Addgene

Showing: 401 - 420 of 14720 results

Results per page:

Sort results by:

pAc5.1C-Fluc-nanos-SRE1,2mut_C

Plasmid

#146019

Purpose

Insect Expression of nanos3UTR-SRE1,2mut

Depositor

Elisa Izaurralde

Insert

nanos3UTR-SRE1,2mut (nos Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1C-Fluc-nanos-SRE1mut_C

Plasmid

#146020

Purpose

Insect Expression of nanos-3UTR-SRE1mut

Depositor

Elisa Izaurralde

Insert

nanos-3UTR-SRE1mut (nos Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTV-mNanog-tdiRFP670-PEST

Plasmid

#122347

Purpose

Targeting vector for mouse Nanog to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for mouse Nanog

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Mouse Nanog locus 3' probe

Plasmid

#60037

Purpose

Cloned genomic fragment of murine Nanog locus used as probe for Southern Blot analysis

Depositor

Jacob Hanna

Article

Maza et al Nat Biotechnol. 2015 Jun 22. doi: 10.103

Insert

Nanog (Nanog Mouse)

Use

Unspecified; Cloning

Tags

Expression

Mutation

Promoter

Available since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
pQE-80L MBP-SspB Nano

Plasmid

#60409

Purpose

E. coli expression of SspB Nano

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

SspB nano

Use

Tags

His-MBP-TEV

Expression

Bacterial

Mutation

Y11K and A15E

Promoter

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
HS69 pComb3xss anti-Homer1 nanobody

Plasmid

#134716

Purpose

Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody (Homer1 L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GC1 pComb3xss anti-Gephyrin nanobody

Plasmid

#134720

Purpose

Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Gephyrin (Human) recombinant llama nanobody GC1 (GPHN L. glama (llama))

Use

Tags

6xHis-HA

Expression

Bacterial

Mutation

Promoter

lac

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29-bdSUMO-Anti-ALFA nanobody

Plasmid

#212311

Purpose

Expression of anti-ALFA nanobody

Depositor

Arthur Laganowsky

Article

Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

Insert

anti-ALFA nanobody

Use

Tags

bdSUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64

Plasmid

#203350

Purpose

Template for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

Depositor

Molly Stevens, Christopher Grigsby

Insert

Nano Luciferase

Use

Template for t7-mediated in vitro transcription o…

Tags

Flanked by 5' and 3' UTRs from Xenopus …

Expression

Mutation

Promoter

T7

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBK1340-LV-DUAL-dGFP-NanoLuc-Puro

Plasmid

#223160

Purpose

Expression of destabilized GFP (dGFP) and NanoLuc for shortened in vivo half-life

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

dGFP-P2A-NanoLuc

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSYN-miRFP670nano3-WPRE-hGHpA

Plasmid

#184688

Purpose

Mammalian expression, miRFP670nano3

Depositor

Vladislav Verkhusha, Axel Nimmerjahn

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3

Use

AAV

Tags

Expression

Mutation

Promoter

hSYN

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
HC20 pEYFPN1 anti-Homer1 nanobody

Plasmid

#135220

Purpose

Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody HC20 (Homer1 L. glama (llama))

Use

Tags

6xHis-HA-EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
IC65 pEGFPN1 anti-IRSp53 nanobody

Plasmid

#135227

Purpose

Mammalian expression of anti-IRSp53 (Human) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-IRSp53 (Human) recombinant llama nanobody IC65 (BAIAP2 L. glama (llama))

Use

Tags

6xHis-HA-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-pTau nanobody (A2) with sortase tag

Plasmid

#233218

Purpose

Mammalian epression of anti-pTau nanobody (A2) with a sortase tag for direct dye conjugation.

Depositor

Jeff Lichtman

Article

Han et al bioRxiv 2023.10.24.563674

Insert

Anti-pTau nanobody (A2)

Use

Tags

Sortase tag, His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Nano-lantern(cAMP-1.6)/pRSETB

Plasmid

#53591

Purpose

Nano-lantern-based luminescent cAMP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern-based luminescent cAMP indicator

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc

Plasmid

#117728

Purpose

Integration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc gene

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

NanoLuc

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
AC50 pEGFPN1 anti-AMIGO-1 nanobody

Plasmid

#135225

Purpose

Mammalian expression of anti-AMIGO-1 (Mouse) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-AMIGO-1 (Mouse) recombinant llama nanobody AC50 (Amigo1 L. glama (llama))

Use

Tags

6xHis-HA-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pVQ CMV NanoV1-2a-EGFP ferritin

Plasmid

#79649

Purpose

Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein

Depositor

Jeffrey Friedman

Article

Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

Inserts

transient receptor potential vanillin 1 (Trpv1 Rat)
camelid anti-GFP nanobody
Enhanced green fluorescent protein
mouse ferritin light chain (Ftl1 Mouse)
mouse ferritin heavy chain (Fth1 Mouse)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Nano-lantern(cAMP-1.6)/pcDNA3

Plasmid

#53594

Purpose

Nano-lantern-based luminescent cAMP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern-based luminescent cAMP indicator

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
GFP-CaM-NanoLuc-15aa-uTEVp

Plasmid

#163034

Purpose

FLiCRE protease with luciferin-gating for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

GFP-CaM-NanoLuc-15aa-uTEVp

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge