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Showing: 401 - 420 of 1089 results

  1. pCI-SMN2-skipping-GLuc

    Plasmid
    #218670
    Purpose
    Alternative splicing signaling: signal decrease with Ex7 inclusion increasing
    Depositor
    Jingxin Wang
    Insert
    SMN2 (SMN2 )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    218670-plasmid-map-sequence-id-450976

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  2. pKP1469

    Plasmid
    #160815
    Purpose
    Expression of ExbB protein; the exbB gene replaces tonB gene in BamHI site of pKP325; includes pBAD promoter and optimized RBS from pBAD24-derived pKP660 (ExbB, ExbD)
    Depositor
    Kathleen Postle
    Insert
    exbB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    160815-plasmid-map-sequence-id-316117

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  3. pENTR CMVie-Lifeact-mTagBFP2 L1-R5

    Plasmid
    #84388
    Purpose
    Multisite Gateway entry vector for expression of mTagBFP2-tagged Lifeact in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Gateway
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV immediate early
    Available since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    84388-plasmid-map-sequence-id-169692

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  4. pUPD2 MS2 (GB2665)

    Plasmid
    #160597
    Purpose
    CDS of MS2 protein with B3 nomenclature
    Depositor
    Diego Orzaez
    Insert
    MS2
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160597-plasmid-map-sequence-id-322069

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  5. 2xUAS-1xhsp70-attB

    Plasmid
    #220317
    Purpose
    includes 2 UAS cassettes and 1 heat-shock inducible hsp70 casette
    Depositor
    Erdem Bangi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    220317-plasmid-map-sequence-id-442487

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  6. pENTR CMVie-Lifeact-mCardinal L1-R5

    Plasmid
    #84392
    Purpose
    Multisite Gateway entry vector for expression of mCardinal-tagged Lifeact in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Gateway
    Tags
    mCardinal
    Expression
    Mammalian
    Mutation
    Promoter
    CMV immediate early
    Available since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    84392-plasmid-map-sequence-id-169693

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  7. pUPD2 dCas9 (GB2664)

    Plasmid
    #160596
    Purpose
    CDS of dCas9 with B3 nomenclature
    Depositor
    Diego Orzaez
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160596-plasmid-map-sequence-id-322070

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  8. mP_ILB_Vector 1

    Plasmid
    #232193
    Purpose
    The plasmid vector designed for the expression of four genes in oleaginous yeasts, including Yarrowia lipolytica and Rhodotorula toruloides.
    Depositor
    Matrika Bhattarai
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 3, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    232193-plasmid-map-sequence-id-463610

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  9. pBABEneo mt T Ag

    Plasmid
    #133713
    Purpose
    Large T antigen mutant form in pBABEneo vector for mammalian expression.
    Depositor
    James A. DeCaprio
    Insert
    SV40gp6 (SV40gp6 SV40)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    aa 1-354 of T antigen (including aa 1-130); no p5…
    Promoter
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    133713-plasmid-map-sequence-id-276425

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  10. pGR595

    Plasmid
    #213785
    Purpose
    Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.
    Depositor
    Chang Liu
    Insert
    BadBoy2 TP-DNAP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    213785-plasmid-map-sequence-id-424581

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  11. Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)

    Plasmid
    #51656
    Purpose
    hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrange
    Depositor
    Adam Cohen
    Insert
    Quasar2-mOrange-Chr64-GFP
    Use
    Lentiviral
    Tags
    GFP and mOrange
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    March 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    51656-plasmid-map-sequence-id-319739

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  12. pNHA_OROV_NCP

    Plasmid
    #208937
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus nucleocapsid protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    Nucleocapsid
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted start codon so HA included at N-terminus
    Promoter
    CMV
    Available since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208937-plasmid-map-sequence-id-418963

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  13. PPT1

    Plasmid
    #25205
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PPT1_02 (PPT1 Human)
    Use
    Tags
    His
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pTipi2.1-AZU_SP

    Plasmid
    #229144
    Purpose
    Allows for expression of recombinant antibodies. Includes human azurocidin signal peptide.
    Depositor
    Institute for Protein Innovation (IPI)
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 9, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    229144-plasmid-map-sequence-id-457154

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  15. pGR596

    Plasmid
    #213787
    Purpose
    Integrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette
    Depositor
    Chang Liu
    Insert
    BadBoy2 TP-DNAP1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    See manuscript
    Promoter
    Available since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213787-plasmid-map-sequence-id-424625

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  16. pGR618

    Plasmid
    #213786
    Purpose
    Integrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette
    Depositor
    Chang Liu
    Insert
    BB-Tv TP-DNAP1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213786-plasmid-map-sequence-id-424624

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  17. p5E-mitfa2.1

    Plasmid
    #81234
    Purpose
    zebrafish mitfa promoter entry vector for multisite Gateway cloning
    Depositor
    James Lister
    Insert
    mitfa promoter (mitfa Zebrafish)
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    mitfa, 2.1kb including 5' UTR
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    81234-plasmid-map-sequence-id-157888

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  18. p202 Dync1li1 wt

    Plasmid
    #26450
    Depositor
    Elizabeth Fisher
    Insert
    cytoplasmic dynein light intermediate chain 1 (Dync1li1 Mouse)
    Use
    Subcloning and sequencing
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Nov. 8, 2010
    Availability
    Academic Institutions and Nonprofits only

  19. pFB-CT10HF-LIC

    Plasmid
    #39191
    Purpose
    SGC Baculovirus transfer vector with C-terminal His tag and FLAG tag, preceded by a TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection
    Depositor
    Nicola Burgess-Brown
    Type
    Empty backbone
    Use
    Baculovirus
    Tags
    TEV-His-FLAG
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39191-plasmid-map-sequence-id-136169

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  20. pMSP1D1Q1

    Plasmid
    #97326
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional Q residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Insert
    MSP1D1Q1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of Q residue after T25
    Promoter
    T7
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97326-plasmid-map-sequence-id-182622

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Showing: 401 - 420 of 1089 results