We narrowed to 1,130 results for: ncl
-
Plasmid#40728PurposeModified pcDNA3.1 vector for ligation independent cloning, including a ccdB cassette for eliminating no-insert backgroundDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only
-
pOpen-TthRT
Plasmid#165566PurposeDNA polymerase that efficiently reverse-transcribes RNA in the presence of MnCl2DepositorInsertThermus thermophilus (Tth) RT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUAST-3x attB
Plasmid#220329Purposeincludes 3 standard UAS cassettesDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-mTagBFP2 PuroR
Plasmid#101893PurposeLentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsmTagBFP2ExpressionMutationPromoterCMV immediate earlyAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-mRuby2 PuroR
Plasmid#84386PurposeLentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsmRuby2ExpressionMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLfa[Tc'hsp5'3'UTR]fa
Plasmid#86450PurposeHeat shock-inducible promoter including hsp-UTRs, ubiquitous expression upon heat-shock of any inserted gene of interest in Tribolium; (Shuttle plasmid)DepositorInsertTc'hsp5'3'UTR
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI-SMN2-skipping-GLuc
Plasmid#218670PurposeAlternative splicing signaling: signal decrease with Ex7 inclusion increasingDepositorInsertSMN2 (SMN2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKP1469
Plasmid#160815PurposeExpression of ExbB protein; the exbB gene replaces tonB gene in BamHI site of pKP325; includes pBAD promoter and optimized RBS from pBAD24-derived pKP660 (ExbB, ExbD)DepositorInsertexbB
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR CMVie-Lifeact-mTagBFP2 L1-R5
Plasmid#84388PurposeMultisite Gateway entry vector for expression of mTagBFP2-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGatewayTagsmTagBFP2ExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 MS2 (GB2665)
Plasmid#160597PurposeCDS of MS2 protein with B3 nomenclatureDepositorInsertMS2
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
2xUAS-1xhsp70-attB
Plasmid#220317Purposeincludes 2 UAS cassettes and 1 heat-shock inducible hsp70 casetteDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR CMVie-Lifeact-mCardinal L1-R5
Plasmid#84392PurposeMultisite Gateway entry vector for expression of mCardinal-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGatewayTagsmCardinalExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 dCas9 (GB2664)
Plasmid#160596PurposeCDS of dCas9 with B3 nomenclatureDepositorInsertdCas9
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
mP_ILB_Vector 1
Plasmid#232193PurposeThe plasmid vector designed for the expression of four genes in oleaginous yeasts, including Yarrowia lipolytica and Rhodotorula toruloides.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMarch 3, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBABEneo mt T Ag
Plasmid#133713PurposeLarge T antigen mutant form in pBABEneo vector for mammalian expression.DepositorInsertSV40gp6 (SV40gp6 SV40)
UseRetroviralTagsExpressionMutationaa 1-354 of T antigen (including aa 1-130); no p5…PromoterAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGR595
Plasmid#213785PurposeIntegrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.DepositorInsertBadBoy2 TP-DNAP1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)
Plasmid#51656Purposehsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrangeDepositorInsertQuasar2-mOrange-Chr64-GFP
UseLentiviralTagsGFP and mOrangeExpressionMammalianMutationPromoterhuman synapsinAvailable sinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNHA_OROV_NCP
Plasmid#208937PurposeExpresses N-terminally HA-tagged Oropouche orthobunyavirus nucleocapsid protein from CMV promoter in mammalian cellsDepositorInsertNucleocapsid
UseTagsHAExpressionMammalianMutationDeleted start codon so HA included at N-terminusPromoterCMVAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTipi2.1-AZU_SP
Plasmid#229144PurposeAllows for expression of recombinant antibodies. Includes human azurocidin signal peptide.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits