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pCMV-HA-GASC1

Plasmid

#24214

Purpose

Mammalian expression of human JMJD2C with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

GASC1 (KDM4C Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pCMVTAG-NEMOL329P

Plasmid

#11971

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMOL329P (IKBKG Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Leucine329 to Proline

Promoter

Available since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only
pCMV3Tag8li-hDDX41-HA

Plasmid

#165469

Purpose

Human DDX41 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

DEAD-box helicase 41 (DDX41 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_Ubiquitin

Plasmid

#131258

Purpose

Mammalian expression of ubiquitin with an N-terminal HA tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin C (UBC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV enhancer + CMV promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hDDX50-HA

Plasmid

#165465

Purpose

Human DDX50 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

DExD-box helicase 50 (DDX50 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hHP1BP3-HA

Plasmid

#165468

Purpose

Human HP1BP3 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Heterochromatin protein 1 binding protein 3 (HP1BP3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT2-mApple

Plasmid

#180319

Purpose

mammalian expression of human SEPT2 fused to monomeric Apple

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN2 (SEPTIN2 Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-msfGFP

Plasmid

#180328

Purpose

mammalian expression of human SEPT9_i1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-msfGFP

Plasmid

#180322

Purpose

mammalian expression of human SEPT9_i3 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i5-msfGFP

Plasmid

#180327

Purpose

mammalian expression of human SEPT9_i5 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v7 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_RTN3E-mScarlet3

Plasmid

#216255

Purpose

mammalian expression vector for RTN3E-mScarlet3

Depositor

Edward Avezov

Article

Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

Insert

reticulon 3 isoform e (RTN3 Human)

Use

Tags

mScarlet3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV14_NHP2L1(15.5kD)

Plasmid

#73065

Purpose

expression clone for human NHP2L1 (15.5kD) with C-terminal 3xFLAG-tag

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

NHP2L1 (15.5kD) (SNU13 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_NOPLight-ctr

Plasmid

#195579

Purpose

Expresses the control sensor NOPLight-ctr in mammalian cells

Depositor

Tommaso Patriarchi

Article

Zhou et al Nat Commun. 2024 Jun 25;15(1):5353. doi:

Insert

NOPLight-ctr

Use

Tags

Expression

Mammalian

Mutation

D110A, D130A

Promoter

CMV

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 D1-25-msfGFP

Plasmid

#180333

Purpose

mammalian expression of human SEPT9_i1 D1-25 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 D1-25

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-i5-msfGFP

Plasmid

#180332

Purpose

mammalian expression of human SEPT9_i1-i5 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v7 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1-i5

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-HA Set1

Plasmid

#192219

Purpose

It expresses HA-fused mouse Set1 isoform protein in mammalian cells

Depositor

Hisakazu Ogita

Article

Molla et al Commun Biol. 2022 Oct 7;5(1):1071. doi:

Insert

Set isoform 1 (Set Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-HA Set2

Plasmid

#192220

Purpose

It expresses HA-fused mouse Set2 isoform protein in mammalian cells

Depositor

Hisakazu Ogita

Article

Molla et al Commun Biol. 2022 Oct 7;5(1):1071. doi:

Insert

Set isoform 2 (Set Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_NESPylRS(AF)_hU6tRNAPyl

Plasmid

#223508

Purpose

PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

Depositor

Edward Lemke

Article

Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

Inserts

NES Pyrrolysyl-tRNA Synthetase (AF) (pylS Methanosarcina mazei)
tRNA Pyl

Use

Tags

Expression

Mammalian

Mutation

Y306A; Y384F

Promoter

CVM, SP6 and U6

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_NESPylRS(WT)_hU6tRNAPyl

Plasmid

#223509

Purpose

PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

Depositor

Edward Lemke

Article

Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

Inserts

NES Pyrrolysyl-tRNA Synthetase (pylS Methanosarcina mazei)
tRNA Pyl

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV, SP6 and U6

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7

Plasmid

#180315

Purpose

mammalian expression of human SEPT7 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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