169,691 results
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Plasmid#143702PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only
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PX458_3XHA_dFnCas9_KRAB_T2A_eGFP
Plasmid#200389Purpose3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expressionDepositorInsertPX458_3XHA_dFnCas9_KRAB_eGFP
UseCRISPRTags3xHA-NLS and SV40NLS-KRAB-T2A-EGFPExpressionMammalianPromoterCBHAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-sCAG-GluClα.CreON
Plasmid#196995PurposeCre recombinase dependent expression of GluClv2.0 alpha subunit. GluClα contains a Cerulean tag. When co-expressed with GluClv2.0 beta subunit, agonist (Ivermectin) induces neuronal silencing.DepositorInsertGluClα -Cerulean
UseAAV and Cre/LoxTagsCeruleanPromotershort CAGAvailable SinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGA-blue
Plasmid#196336PurposeEasy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
L1P38
Plasmid#195932PurposeLevel 1 PhiC31 cassette with estradiol inducible promoter: p35S_XVE-PhiC31-tUBQ1DepositorInsertp35S_XVE-PhiC31-tUBQ1
ExpressionPlantAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
T7-TetO-GFP_ColE1
Plasmid#173193PurposeExpresses GFP under the control of a T7-TetO promoter. Expression can be repressed via TetR, and repression can be relieved via addition of tetracycline. Contains the ColE1 origin of replication.DepositorInsertT7-TetO
TagsssrA degradation tag (DAS+4)ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF688_isoform1
Plasmid#88779PurposeDonor Vector containing ZNF688 transcription factor, part of the Human TFome CollectionDepositorInsertZNF688 (ZNF688 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow foā¦Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTJK438
Plasmid#138004Purpose3xMYC-SIX1 expression. Vector also expresses GFP under control of hUBC promoterDepositorAvailable SinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-G-S10
Plasmid#127829PurposeProduction of recombinant human ribosomal protein S10DepositorAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBP-BBa_B0015
Plasmid#72998PurposeBBa_B0015 iGEM terminator (5' TCGA/ 3' TGTT fusion)DepositorInsertBBa_B0015 terminator
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesiā¦Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRPa-iSL(MCS3+4)
Plasmid#69246PurposeTet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiPromoterRRNA promoterAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGLO-Blue
Plasmid#220782PurposeExpresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)DepositorInsertblue chromoprotein (aeBlue)
Tags8xHisExpressionBacterialAvailable SinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tn7 helper plasmid
Plasmid#141161PurposeTemperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoterDepositorInsertTn7 transposon machinery
ExpressionBacterialPromoterArabinose-inducible pBADAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE3
Plasmid#90337PurposeAHR - Aryl hydrocarbon receptor gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterAHRAvailable SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-AUP1-FLAG
Plasmid#185336PurposeMammalian expression of AUP1DepositorAvailable SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNX32-Dest
Plasmid#105083PurposeYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloningDepositorTypeEmpty backboneTagsNubGExpressionYeastAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-Tol2 transposase
Plasmid#158774PurposeExpression of Tol2 transposaseDepositorInsertTol2 tranposase
ExpressionMammalianMutationTol2 codon usage frequency well suitable for muriā¦Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-NLS-HA-TurboID
Plasmid#215075PurposeExpresses NLS-HA-TurboID in mammalian cells from a lentiviral vector.DepositorAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-dTAG-Puro
Plasmid#207727PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
M13cp-dg2 hp
Plasmid#218094PurposeHelper plasmid that allows phagemid production upon complementation with a phagemid expressing pIIDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only