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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,400 results for: Coli

Showing: 6271 - 6300 of 9400 results

  1. pMZ1001

    Plasmid
    #204398
    Purpose
    A pC-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.
    Depositor
    Lauren Andrews
    Article
    Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102
    Inserts
    quorum sensing regulator RpaR (rpaR )
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PftsH and Synthetic promoter PRpa0002
    Available Since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    204398-plasmid-map-sequence-id-405813

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  3. pMH-macroHC

    Plasmid
    #82337
    Purpose
    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli
    Depositor
    Michael Hothorn
    Article
    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p
    Insert
    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    Use
    Tags
    6xHis non-cleavable and macro domain of human his…
    Expression
    Bacterial
    Mutation
    Truncated protein, amino acids 1-178
    Promoter
    Available Since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    82337-plasmid-map-sequence-id-155830

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  12. pRL1124

    Plasmid
    #70690
    Purpose
    provides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.
    Depositor
    Peter Wolk
    Article
    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.
    Inserts
    avaiM
    eco47iiM
    ecot22iM
    Use
    Bacterial shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    70690-plasmid-map-sequence-id-126465

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  15. pET28a-HsSENP7

    Plasmid
    #71470
    Purpose
    Encodes human full length SENP7. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 7 (SENP7 Human)
    Use
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available Since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  24. pSB1A2QS

    Plasmid
    #138268
    Purpose
    MINPA Gene circuit expression in E. coli. GFP serves as a reporter for Plux/tet promoter activity. mCherry serves as reporter for Para/lac promoter activity
    Depositor
    Xiao Wang
    Article
    Wu et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife
    Inserts
    AraC-TetR operon
    LuxR-LacI operon
    mCherry
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Para/lac and Plux/tet
    Available Since
    April 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    138268-plasmid-map-sequence-id-288132

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  28. pPD3C

    Plasmid
    #86971
    Purpose
    Expresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA3 fused to phosphite dehydrogenase and P450cam
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, N106R in PCNA3, silent mutat…
    Promoter
    T7 promoter
    Available Since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86971-plasmid-map-sequence-id-173928

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  30. pfMDH_H303A

    Plasmid
    #67730
    Purpose
    Expresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed histidine 303 to alanine
    Promoter
    T7
    Available Since
    Sept. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 6271 - 6300 of 9400 results