We narrowed to 65,473 results for: nin

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xCas9(3.6)-BE4

Plasmid

#108386

Purpose

Mammalian expression vector for xCas9(3.6)-BE4

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.6)-BE4

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-Akt1-K179M

Plasmid

#73409

Purpose

Expresses kinase-inactive mutant of Akt with HA tag

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Tags

HA tag

Expression

Mammalian

Mutation

changed K179M to make Kinase inactive mutant

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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Notch1 intracellular domain-pcw107

Plasmid

#64621

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Mutation

intracellular domain *

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pLX304-YAP1 _TA

Plasmid

#59143

Purpose

YAP1 mutants that harbor a deletion of the transcriptional activation domain

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

TA (transactivation) domain deleted; aa291-497

Promoter

CMV

Available Since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Flag mHMGB2

Plasmid

#31610

Depositor

Yasuhiko Kawakami

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2009 Sep 29. 1

Insert

HMGB2 (Hmgb2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-FGR

Plasmid

#23877

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FGR (FGR Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-ABEmax-NRTH

Plasmid

#136922

Purpose

Expresses ABEmax-NRTH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

ABEmax-NRTH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pJZC78

Plasmid

#62339

Purpose

sgRNA + 1x COM with COM-KRAB effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1x COM binding module
COM-KRAB

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…

Promoter

CMV and U6

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pDESTN-MTp-p53-Venus

Plasmid

#53736

Purpose

Expresses Venus tagged p53 under the metallothionein promoter

Depositor

Galit Lahav

Article

Batchelor et al Mol Cell. 2008 May 9;30(3):277-89. doi:

Insert

p53 (TP53 Human)

Tags

Venus

Expression

Mammalian

Promoter

metallothionein promoter

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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DDB1

Plasmid

#25246

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

DDB1_1 (DDB1 Human)

Use

Baculovirus expression

Tags

His

Expression

Insect

Promoter

polyhedrin

Available Since

Aug. 13, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-N-Flag-HA-GFP

Plasmid

#41034

Purpose

Retroviral expression of GFP in mammalian cells

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

GFP

Use

Gateway Cloning and Retroviral

Tags

Flag, HA, and IRES Puro

Expression

Mammalian

Promoter

LTR-driven expression

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only
pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

Plasmid

#191797

Purpose

vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

Depositor

Huawei Tong

Article

Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

Insert

humanized dCas13d

Use

CRISPR

Expression

Mammalian

Mutation

R239A, H244A, R858A, H863A

Promoter

CBh, SV40, hU6

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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miniCGBE1 (pBM1219)

Plasmid

#140253

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

BE4max(R33A)-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in Cas9

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-mt-mKeima-P2A-FRB-Fis1

Plasmid

#135295

Purpose

stably express both mKeima and FRB-Fis1 for CID induced mitophagy in mammalian cells

Depositor

Richard Youle

Article

Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

Insert

mt-mKeima-P2A-FRB-Fis1 (FIS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

mt-mKeima is fused with FRB-Fis1 via P2A seqeunce

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTRANS_221

Plasmid

#91115

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)

Plasmid

#71485

Purpose

Base plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon arms

Depositor

Richard Sherwood

Article

Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

Type

Empty backbone

Promoter

U6

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-Calr-KDEL-IRES-Puromycin

Plasmid

#134664

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

calreticulin (CALR Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 18-413

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV/myc/mito-mutant-hogg1

Plasmid

#18708

Depositor

David Sidransky

Article

Chatterjee et al BMC Cancer. 2006 Oct 3;6:235. doi: 10.11

Insert

8-oxoguanine DNA glycosylase (OGG1 Human)

Expression

Mammalian

Mutation

Amino acid 229, Arginine to Glutamine

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
PB-TRE3G-MYCN

Plasmid

#104542

Purpose

PiggyBac vector encoding doxycycline-inducible human MYCN

Depositor

David Vereide

Article

Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

Insert

MYCN proto-oncogene, bHLH transcription factor (MYCN Human)

Expression

Bacterial and Mammalian

Promoter

TRE3G

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Smad1 (AAVA)

Plasmid

#14957

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

Insert

Smad1 (AAVA) (SMAD1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Three Serine to Alanine mutations in the C-termin…

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only