We narrowed to 29,541 results for: REP

1. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

   Plasmid

   #170186

   Purpose

   This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

   Depositor

   Bradley Bernstein

   Article

   Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

   Insert

   CreERT2-T2A-eBFP2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1 alpha

   Available since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV sMTase (eGFP)

   Plasmid

   #89930

   Purpose

   Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.

   Depositor

   Carl Novina

   Article

   Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

   Insert

   dCas9-MC-IRES-MN
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   deactivated Cas9

   Promoter

   CMV

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 3xFLAG-CUL1-pCMV7.1

   Plasmid

   #155019

   Purpose

   N-terminal 3xFLAG-tagged CUL1 in a pCMV7.1 vector

   Depositor

   Andre Catic

   Article

   Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

   Insert

   Cullin 1 (CUL1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   pCMV7.1

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 3xFLAG-CUL3-pCMV7.1

   Plasmid

   #155021

   Purpose

   N-terminal 3xFLAG-tagged CUL3 in a pCMV7.1 vector

   Depositor

   Andre Catic

   Article

   Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

   Insert

   Cullin 3 (CUL3 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   pCMV7.1

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRK172-6xHis-0N4R-P301S-tau-HiBiT

   Plasmid

   #187835

   Purpose

   Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

   Depositor

   William McEwan

   Article

   Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

   Insert

   6XHis-0N4R-P301S-tau-HiBiT
   

   Use

   Tags

   6xHistidine and HiBiT

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPBbsr-mKO-MK2

   Plasmid

   #115492

   Purpose

   Expression vector for the p38 activity reporter mKO-MK2 in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

   Insert

   mKO-MK2 (MAPKAPK2 Human)
   

   Use

   Tags

   monomeric Kusabira Orange (mKO)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBS-KS-attB2-SA(2)-T2A-Gal4DBD-Hsp70

   Plasmid

   #62904

   Purpose

   Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4DBD-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBS-KS-attB2-SA(2)-T2A-LexA::QFAD-Hsp70

   Plasmid

   #62949

   Purpose

   Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 2

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-LexA::QFAD-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-FLEX-SaCas9-U6-sgSlc17a6

   Plasmid

   #124847

   Purpose

   Mutagenesis of Slc17a6 with SauCas9

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a6 gRNA (Slc17a6 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BCAM promoter-pRB-Puro-TurboRFPmyc-T2A-Luciferase

    Plasmid

    #188391

    Purpose

    Reporter for BCAM expression

    Depositor

    Markus Frank

    Article

    Sasamoto et al Cell Rep. 2022 Aug 9;40(6):111166. doi:

    Insert

    BCAM reporter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. phage UbiC NLS HA stdMCP stdHalo

    Plasmid

    #104999

    Purpose

    Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labeling

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    stdMCP-stdHalo
    

    Use

    Lentiviral

    Tags

    HA tag, NLS, Two synonymous copies of the Halo-ta…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    UbiC

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-VTKS2-TVA-eGFP-N2cG

    Plasmid

    #175439

    Purpose

    Cre-on (DIO) helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

    Depositor

    Gordon Fishell

    Article

    Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

    Insert

    TVA-P2A-eGFP-P2A-N2cG
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FLAG-FullLength-TET1-WT(21)

    Plasmid

    #124396

    Purpose

    Full-length TET1 used to PiggyBAC rescue the function of TET1/2 DKO cells

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    FLAG-FullLength-TET1 (TET1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    full length protein is 2136 aa

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pmScarlet-HSP70

    Plasmid

    #163790

    Purpose

    Expresses mScarlet-Hsp70 in mammalian cells

    Depositor

    Vincent Timmerman

    Article

    Adriaenssens et al Sci Rep. 2020 May 29;10(1):8755. doi: 10

    Insert

    HSP70 (HSPA1A Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. psigma2-EGFP

    Plasmid

    #53610

    Purpose

    Expresses sigma2 of adaptor protein of AP2

    Depositor

    Tom Kirchhausen

    Article

    Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

    Insert

    sigma2 (Ap2s1 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.2-Cx43-HA

    Plasmid

    #49851

    Purpose

    Encodes human connexin 43 with a C-terminal HA epitope fusion tag

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Contains several wobble mutations to confer resis…

    Promoter

    CMV

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENTR221-JUN

    Plasmid

    #79512

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human JUN CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    JUN (JUN Human)
    

    Use

    Multisite gateway entry clone

    Tags

    HA Tag

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-FLEX-SaCas9-U6-sgGrin2b

    Plasmid

    #124851

    Purpose

    Mutagenesis of Grin2b

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin2b (Grin2b Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pK168.AAV-EF1α-DIO-tTA-P2A-RFP-WPRE (Supernova)

    Plasmid

    #85039

    Purpose

    For AAV-TRE-Cre based Supernova-RFP, pK168 should be used with pK170.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    RFP-P2A-tTA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-eCas9

    Plasmid

    #140237

    Purpose

    Lentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffold

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a core

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge