We narrowed to 9,412 results for: pho;

1. sgRNA3_NANOG

   Plasmid

   #64155

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA3_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgRNA2_NANOG

   Plasmid

   #64154

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA2_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUG-PtenN276S

   Plasmid

   #140737

   Purpose

   Lentiviral vector to overexpress the PTEN mutation N276S as a GFP-PTEN fusion protein

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   PTEN – phosphatase and tensin homolog (PTEN Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N276S

   Promoter

   hUbiC

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNeonGreen-C1-Nir1-LNS2

   Plasmid

   #220083

   Purpose

   High affinity PA biosensor with green fluorophore (also known as NeonGreen-PILS-Nir1)

   Depositor

   Gerry Hammond

   Article

   Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

   Insert

   PITPNM3 (PITPNM3 Human)
   

   Tags

   NeonGreen-GGSGGM

   Expression

   Mammalian

   Mutation

   amino acids 613-897

   Promoter

   CMV

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. mClY-N1

   Plasmid

   #90457

   Purpose

   A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

   Depositor

   Joseph Santos-Sacchi

   Article

   Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

   Insert

   mClY-N1
   

   Expression

   Mammalian

   Mutation

   EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF0144

   Plasmid

   #141536

   Purpose

   Lentiviral vector for overexpressing the PHOX2A transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PHOX2A (PHOX2A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCherry-C1-Nir1-LNS2

   Plasmid

   #220084

   Purpose

   high affinity PA biosensor with red fluorophore (also known as mCherry-PILS-Nir1)

   Depositor

   Gerry Hammond

   Article

   Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

   Insert

   PITPNM3 (PITPNM3 Human)
   

   Tags

   mCherry-GGSGGM

   Expression

   Mammalian

   Mutation

   amino acids 613-897

   Promoter

   CMV

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pF4Ag NanoLuc (ATG-42)

   Plasmid

   #137777

   Purpose

   Expresses NanoLuc in multi promoter expression vector (CMV andT7 promoters)

   Depositor

   Lance Encell

   Article

   Hall et al ACS Chem Biol. 2012 Nov 16;7(11):1848-57

   Insert

   NanoLuc (Nluc)
   

   Expression

   Bacterial and Mammalian

   Mutation

   NanoLuc contains 16 amino acid changes compared t…

   Promoter

   T7 and CMV

   Available Since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. opto-E-cad GFP

   Plasmid

   #203327

   Purpose

   Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells

   Depositor

   Seraphine Wegner

   Article

   Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi:

   Insert

   Photoswitchable - E-cadherin with GFP tag (CDH1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Addition of Aslov2 domain at T133 of human E-cadh…

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTriEx-mCherry-PA-Rac1

    Plasmid

    #22027

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    

    Tags

    6xHis and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJM656

    Plasmid

    #53086

    Purpose

    A non-Phosphatidylserine binding mutant form of Lactadherin(sGFP::LactC1C2mut) to be used as a control

    Depositor

    Ding Xue

    Article

    Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

    Insert

    Lactaherin (Mfge8 Mouse)
    

    Tags

    GFP (w/ introns) and signal sequence

    Expression

    Worm

    Mutation

    deleted native signal sequence, deleted C-termina…

    Promoter

    hsp-16.41

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTriEx-PA-Rac1

    Plasmid

    #22024

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    

    Tags

    6X His

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-ALK3 wt

    Plasmid

    #80873

    Purpose

    mammalian expression of ALK3 WT

    Depositor

    Aristidis Moustakas

    Article

    Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

    Insert

    ALK3 (BMPR1A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSFDuet-v2 RsTAL At4CL

    Plasmid

    #126524

    Purpose

    expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

    Depositor

    Andrew Woolley

    Article

    Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

    Inserts

    tyrosine ammonia lyase
    p-coumaroyl-CoA ligase (4CL1 Mustard Weed)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21a-C2m2-SNAP

    Plasmid

    #208773

    Purpose

    To express C2m2-SNAP in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2m2-SNAP (Mfge8 Mouse)
    

    Tags

    SNAP-tag

    Expression

    Bacterial

    Mutation

    Mutated 24 and 45 lysine to asparagine in C2 doma…

    Promoter

    T7 promoter

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTriEx-mVenus-PA-Rac1

    Plasmid

    #22007

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    

    Tags

    6X His and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV(Leu404-Leu546), Rac1 starts at I4 and contain…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-FLAG-HA-CAD S1859A

    Plasmid

    #46239

    Purpose

    FLAG-HA-CAD with S1859 phosphorylation site mutated to alanine

    Depositor

    Brendan Manning

    Article

    Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

    Insert

    carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Serine 1859 changed to Alanine (S1859A)

    Promoter

    cmv

    Available Since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pLKO.1-shLuc.mKO2

    Plasmid

    #85224

    Purpose

    shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21a-C2m2-mKate

    Plasmid

    #208765

    Purpose

    To express C2m2-mKate in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2m2-mKate (Mfge8 Mouse)
    

    Tags

    mKate

    Expression

    Bacterial

    Mutation

    Mutated 24 and 45 lysine to asparagine in C2 doma…

    Promoter

    T7 promoter

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEB1N-LZ-LOV2(wt)

    Plasmid

    #107614

    Purpose

    N-terminal half of π-EB1 with wild-type LOV2, no fluorescent tag

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 (MAPRE1 Human)
    

    Tags

    A. sativa phototropin 1 LOV2 domain and GCN4 leuc…

    Expression

    Mammalian

    Mutation

    EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

    Promoter

    CMV

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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