We narrowed to 7,704 results for: alp

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pAAV-Syn1-DIO-Snca (human A53T)

Plasmid

#214201

Purpose

For Cre-dependent expression of alpha synuclein (human gene containing A53T mutation)

Depositor

Thomas Hnasko

Article

Steinkellner et al Brain. 2022 Apr 29;145(3):879-886. doi:

Insert

Snca (A53T) (SNCA Human)

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

A53T

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFPC2-gephyrin S268E/270E

Plasmid

#199609

Purpose

Encodes N-terminal eGFP-tagged P1-gephyrin S268/270E for expression in mammalian cells.

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

Insert

Gephyrin (Gphn Rat)

Tags

EGFP

Expression

Mammalian

Mutation

S268E/S270E

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-H1DBD-H2

Plasmid

#200205

Purpose

Expression of HSF2 with HSF1 DBD

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

Halo-H1DBD-H2 (Hsf1, Hsf2 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

HSF1 (1-399); HSF2 (366-1554)

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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H2DBD-H1-Halo

Plasmid

#200193

Purpose

Expression of HSF1 with HSF2 DBD

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

H2DBD-H1-Halo (Hsf1, Hsf2 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

HSF2 (1-366); HSF1 (399-1576)

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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IL2-mCherry-rGBD in pmCherryN1

Plasmid

#187286

Purpose

Express IL2-Rhotekin GTPase-binding domain-mCherry

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, Rhotekin (IL2, Rtkn Human, Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMIR218-2_nt5G>U

Plasmid

#174009

Purpose

miR218 nt5 point mutation in SLIT3

Depositor

Andrew M. Siedlecki

Article

Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

Insert

SLIT3 (SLIT3, MIR218-2 Human)

Expression

Mammalian

Mutation

NT5 G>U point mutation in SLIT3

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-734pUC19-tNGFR-P2A-PIK3R1

Plasmid

#186107

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

PIK3R1 (PIK3R1 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b MOSPD2 [315-445]

Plasmid

#186474

Purpose

Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Tags

6HIS

Expression

Bacterial

Mutation

MSP domain [315-445]

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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NSS-GFP

Plasmid

#163661

Purpose

Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from TNF and TMD and luminal domain from ST6GAL1 in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)

Tags

GFP

Expression

Mammalian

Mutation

This chimera contains the cytosolic tail from TNF…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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SNN-GFP

Plasmid

#163664

Purpose

Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, and TMD and luminal domain from TNF in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)

Tags

GFP

Expression

Mammalian

Mutation

This chimera contains the cytosolic tail from ST,…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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NNS-GFP

Plasmid

#163666

Purpose

Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail and TMD from TNF, and luminal domain from ST6GAL1 in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)

Tags

GFP

Expression

Mammalian

Mutation

This chimera contains the cytosolic tail from TNF…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-EGFP-Bin1b

Plasmid

#176021

Purpose

Leishmania cell free expression of zebrafish EGFP-Bin1b. Parton lab clone GRS

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1b (bin1b Zebrafish)

Use

Leishmania cell free expression

Tags

EGFP

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Cavin4a-mCherry

Plasmid

#176010

Purpose

Mammalian expression of zebrafish Cavin4a-mCherry. Parton lab clone HYM

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4a (cavin4a Zebrafish)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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QuasAr-P2A-hKvb1

Plasmid

#154083

Purpose

Independent expression of QuasAr and human Kvbeta 1 in pFCK vector

Depositor

Michael Hoppa

Article

Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

Insert

QuasAr-P2A-human Kvb1 (KCNAB1 Human, Synthetic)

Use

Lentiviral

Tags

myc-Flag

Expression

Mammalian

Promoter

CaMKII

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2(delta)KL-VSV

Plasmid

#139352

Purpose

Expression of TYK2 protein missing the kinase-like (pseudokinase) domain

Depositor

Sandra Pellegrini

Article

Velazquez et al J Biol Chem. 1995 Feb 17;270(7):3327-34.

Insert

TYK2 cDNA deleted of aa594-877 with a Tyr inserted after aa593 (TYK2 Human)

Tags

VSV

Expression

Mammalian

Mutation

V362F-deletion of aa 594-877

Promoter

CMV

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pZIP-hCMV-ZsGreen-P2A-Puro-IKZF3 sh1

Plasmid

#123326

Purpose

Lentiviral vector for expression of shRNA targeting IKZF3 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

shRNA targeting IKZF3 embedded in miR30 backbone (IKZF3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pZIP-hCMV-ZSGreen-P2A-Puro-IKZF3 sh2

Plasmid

#123327

Purpose

Lentiviral vector for expression of shRNA targeting IKZF3 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

shRNA targeting IKZF3 embedded in miR30 backbone (IKZF3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLuc-Optop38i-lsm

Plasmid

#89747

Purpose

Expression of light-regulated p38 inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor MK3BD3-13F

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

Optop38i lit-state mutant

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

I539E mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLuc-OptoJNKi-lsm

Plasmid

#89746

Purpose

Expression of light-regulated JNK inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi lit-state mutant

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

I539E mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLuc-OptoJNKi-dsm

Plasmid

#89745

Purpose

Expression of light-regulated JNK inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi dark-state mutant

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

C450A mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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