We narrowed to 24,461 results for: nes

1. pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR)

   Plasmid

   #178985

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCRE-iRFP670

   Plasmid

   #82696

   Purpose

   Expresses CRE and iRFP670 under two separate PGK promoters.

   Depositor

   Alan Mullen

   Article

   Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

   Insert

   CRE, iRFP670
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pETM6-RgTALsyn-HpaBC

   Plasmid

   #100949

   Purpose

   Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Inserts

   RgTALsyn
   HpaB
   HpaC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   T7

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CMV-T7-hOsa1

   Plasmid

   #17986

   Depositor

   Naoko Tanese

   Article

   Inoue et al J Biol Chem. 2002 Nov 1. 277(44):41674-8

   Insert

   Osa1 (ARID1A Human)
   

   Use

   Tags

   T7 epitope

   Expression

   Mammalian

   Mutation

   Missing 5' end

   Promoter

   Available since

   Aug. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

5. KCC2B_HUMAN_D0

   Plasmid

   #79718

   Purpose

   This plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   KCC2B
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pSLIK 3xFLAG-KIF20A zeo

   Plasmid

   #192322

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-KIF20A

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   KIF20A (KIF20A Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pB-RTP2-Integration

   Plasmid

   #129459

   Purpose

   piggyBac transposon vector that Inducibly (Tet-On) expresses RTP2

   Depositor

   Sriram Kosuri

   Article

   Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

   Insert

   RTP2 (RTP2 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE (Tet-On)

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)

   Plasmid

   #178989

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p2xp53_RE

   Plasmid

   #118053

   Purpose

   GBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   2 copies of the p53 DNA binding motif
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p4xSMAD_RE

    Plasmid

    #118051

    Purpose

    GBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    4 copies the SMAD DNA binding motif
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MK14_HUMAN_D0

    Plasmid

    #79680

    Purpose

    This plasmid encodes the kinase domain of MK14. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    MK14
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pgRGFP

    Plasmid

    #82695

    Purpose

    Expresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.

    Depositor

    Alan Mullen

    Article

    Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK, U6

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET15b-eGO-Matryoshka-LE-LP

    Plasmid

    #100017

    Purpose

    Bacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    cpEGFP-LSSmOrange
    

    Use

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Leu and Glu N-term linker and Leu and Pro C-term …

    Promoter

    T7

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MSCV FLIPi P2G_Thy1.1 PTEN

    Plasmid

    #19749

    Depositor

    Richard Hynes

    Article

    Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

    Insert

    oligo targeting PTEN (Pten Mouse)
    

    Use

    Cre/Lox, RNAi, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAEL007097-Cas9

    Plasmid

    #100705

    Purpose

    The promoter of AAEL007097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL007097-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Mutation

    Promoter

    AAEL007097 and Opie2

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LMP FFB

    Plasmid

    #89491

    Purpose

    Firefly hairpin

    Depositor

    Russell Jones

    Article

    Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

    Insert

    Firefly luciferase hairpin, clone B
    

    Use

    Mouse Targeting and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (The GFP-Puro is from a PGK promoter; the shRN…

    Available since

    May 25, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pLX302 IL10RA-V5 puro

    Plasmid

    #47552

    Purpose

    Lentiviral expression of V5-tagged IL10RA in mammalian cells

    Depositor

    Kevin Janes

    Article

    Kang et al Sci Signal. 2013 Aug 6;6(287):rs13. doi:

    Insert

    IL10RA (IL10RA Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pEN_TT 3xFLAG-KIF20A

    Plasmid

    #192297

    Purpose

    Gateway entry vector for an inducible 3xFLAG-KIF20A

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    KIF20A (KIF20A Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-4T-1 3xHA-MKK6-EE

    Plasmid

    #47579

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MKK6-EE (MAP2K6 Human)
    

    Use

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Ser207 to Glu, Thr211 to Glu

    Promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

20. MSCV FLIPi P2G_Thy1.1 p53

    Plasmid

    #19748

    Depositor

    Richard Hynes

    Article

    Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

    Insert

    oligo targeting p53 (Trp53 Mouse)
    

    Use

    Cre/Lox, RNAi, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge