We narrowed to 11,766 results for: nsf

1. TFRC-His-bio

   Plasmid

   #52338

   Purpose

   Expresses full-length Transferrin receptor protein 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   TFRC (TFRC Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-syn-jYCaMP1

   Plasmid

   #135423

   Purpose

   Yellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfection

   Depositor

   Kaspar Podgorski

   Article

   Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

   Insert

   jYCaMP1
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-PRMT6(V86K/D88A)

   Plasmid

   #164698

   Purpose

   Expression of PRMT6(V86K/D88A) with an N-terminal FLAG tag

   Depositor

   Cheryl Arrowsmith

   Article

   Eram et al ACS Chem Biol. 2016 Mar 18;11(3):772-781

   Insert

   PRMT6 (PRMT6 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   V86K, D88A

   Promoter

   CMV

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-EF1-TfR20-SNAP-IRES-Puro

   Plasmid

   #171018

   Purpose

   Lentiviral expression of a transferrin receptor-SNAP-tag fusion in mammalian cells

   Depositor

   Angus Johnston

   Article

   FitzGerald et al Nat Commun. 2020 Sep 8;11(1):4482. doi:

   Insert

   Transferrin receptor (Human) - SNAP-tag (TFRC Human)
   

   Use

   Lentiviral

   Tags

   SNAPtag and TfR

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 EWS-myc-HIS

   Plasmid

   #46386

   Purpose

   expresses myc, HIS tagged EWSR1 in mammalian cells

   Depositor

   Heinz Gehring

   Article

   Pahlich et al Proteins. 2008 Sep;72(4):1125-37. doi: 1

   Insert

   EWSR1 (EWSR1 Human)
   

   Tags

   His and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

6. AAV-short_BrokenHeart (no BC)

   Plasmid

   #86950

   Purpose

   AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.

   Depositor

   Rob Mitra, Joseph Dougherty

   Article

   Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   Brokenheart construct
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC-BAR-YR

   Plasmid

   #61766

   Purpose

   Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).

   Depositor

   Ken Haynes

   Article

   Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

   Inserts

   Bar gene
   2 micron origin or replication and URA3 gene for S. cerevisiae
   

   Use

   Ternary vector for agrobacterium mediated transfo…

   Promoter

   trpC promoter

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-TdT

   Plasmid

   #126450

   Purpose

   Expresses human TdT in mammalian cells. (pTBL206)

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   TdT (DNTT Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFPV25-cR-2iG

   Plasmid

   #204618

   Purpose

   Fluorescent reporter for SPI2 induction in Salmonella

   Depositor

   Jacques Neefjes

   Article

   Stévenin et al Cell Rep. 2024 Nov 1;43(11):114931. doi:

   Inserts

   rpsM promoter
   sseA promoter
   

   Tags

   GFP and dsRed

   Expression

   Bacterial

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti_EGFP-P2A-HAUS6_IRES_Blast

    Plasmid

    #182886

    Purpose

    Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (WT full length)

    Depositor

    Daniel Gerlich

    Article

    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168

    Insert

    HAUS 6 (HAUS6 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Bacterial and Mammalian

    Promoter

    EF1alpha core

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-EF1a-SPdCas9-DNMT3A-2A-Blast

    Plasmid

    #71216

    Purpose

    dCas9 fused to human DNMT3A catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3A catalytic domain
    

    Use

    Lentiviral

    Tags

    dSpCas9-DNMT3A

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pD40-His/V5-c-MycS62A

    Plasmid

    #45599

    Depositor

    Rosalie Sears

    Article

    Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

    Insert

    c-Myc S62A (Myc Mouse)
    

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    S62A

    Promoter

    CMV

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pBabe puro H-Ras 61L

    Plasmid

    #13485

    Depositor

    Channing Der

    Article

    Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

    Insert

    HRas 61L (HRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    61L

    Available Since

    Dec. 20, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pLB052 - deAct - SpVB

    Plasmid

    #136286

    Purpose

    entry vector for GreenGate cloning method, D module, open reading frame of the mono(ADP-ribosyl) transferase domain of the Salmonella enterica SpvB

    Depositor

    Alexis Maizel

    Article

    Vilches Barro et al Curr Biol. 2019 Aug 5;29(15):2443-2454.e

    Insert

    deAct - SpvB
    

    Expression

    Bacterial

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-syn-jYCaMP1s

    Plasmid

    #135424

    Purpose

    Yellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfection, slow variant

    Depositor

    Kaspar Podgorski

    Article

    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

    Has Service

    AAV1

    Insert

    jYCaMP1s
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW57.1-Cdx2-3xT7

    Plasmid

    #200891

    Purpose

    Inducible lentiviral expression of Cdx2

    Depositor

    Kai Ge

    Article

    Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

    Insert

    Cdx2 (Cdx2 Mouse)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    3xT7

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. LV-hHOXA13

    Plasmid

    #135561

    Purpose

    Express hHOXA13 and EGFP in mammalian cells

    Depositor

    Jose Teixeira

    Article

    George et al Cell Rep. 2019 Dec 17;29(12):4069-4085.e

    Insert

    Homeobox A13 (HOXA13 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CBH

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-IpgD(C439S)

    Plasmid

    #183667

    Purpose

    N-terminally tagged Shigella flexneri IpgD (catalytically inactive) for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    IpgD
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C439S

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXPE

    Plasmid

    #15284

    Depositor

    Ron Vale

    Article

    Navone et al J Cell Biol. 1992 Jun . 117(6):1263-75.

    Insert

    ubiquitous kinesin heavy chain (KIF5B Human)
    

    Expression

    Bacterial

    Mutation

    The insert is missing the 1st 180 bp of 5' u…

    Available Since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pGem-nHyPer:cAPX

    Plasmid

    #84736

    Purpose

    Simultaneous expresion of stromal HyPer2 and cytosolic Ascorbate peroxidase (APX) in plant cells

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Inserts

    APX1 (APX1 Mustard Weed)
    HyPer2 targeted to nucleus
    

    Tags

    NLS-SV40

    Expression

    Plant

    Promoter

    pFMV/p35S

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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