We narrowed to 58,587 results for: ran

1. pHDM-SARS-CoV-2 Δ18

   Plasmid

   #164434

   Purpose

   Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   SARS-CoV-2 Spike truncated to remove 18 amino acids (S )
   

   Expression

   Mammalian

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Plasmid

   #176534

   Purpose

   Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associated IscB (Delaware Bay metagenome)
   

   Tags

   14xHis-MBP-bdSUMO and TwinStrep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEXP5-aHL_spytag_his

   Plasmid

   #107334

   Purpose

   Monomeric SpyTag-6×-His alpha-hemolysin

   Depositor

   George Church

   Article

   Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

   Insert

   alpha-hemolysin-SpyTag-6xHis
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

   Plasmid

   #171090

   Purpose

   CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Sth1 dCas9
   Chloramphenicol acetyltransferase
   TagBFP
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

   Mutation

   D9A, H599A

   Promoter

   TUB1 (Toxoplasma gondii), transcriptionally linke…

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57.1_SPTLC1_C133W

   Plasmid

   #181921

   Purpose

   3rd generation, Inducible lentiviral plasmid for expression of human mutant SPTLC1 C133W

   Depositor

   Christian Metallo

   Article

   Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10

   Insert

   Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1) mutant C133W (SPTLC1 Human)
   

   Use

   Lentiviral; Mammalian expression, , doxycycline i…

   Expression

   Mammalian

   Mutation

   cysteine at position 133 of the coding region of …

   Promoter

   tight TRE promoter

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-FLAG-puro-PTPIP51(1-150_175-470)

   Plasmid

   #170537

   Purpose

   Expresses PTPIP51 FFAT-like motif (151-175) deletion mutant (1-150_176-470) in human HeLa cells after transfection

   Depositor

   Byung Il Lee

   Article

   Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

   Insert

   Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
   

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   deleted amino acids 151-175

   Promoter

   CAG promoter

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

7. pJT410 AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA

   Plasmid

   #202059

   Purpose

   Inducible AAV activator

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA
   

   Use

   AAV; Aav with reporter

   Expression

   Mammalian

   Mutation

   ITRs intact

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1a-FLuc-WPRE-HGHpA

   Plasmid

   #87951

   Purpose

   Recombinant single-stranded AAV transfer vector expressing Firefly Luciferase under the EF1a promoter

   Depositor

   Mark Kay

   Article

   Paulk et al Mol Ther. 2017 Sep 25. pii: S1525-0016(1

   Insert

   Firefly Luciferase
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pROS11

   Plasmid

   #107925

   Purpose

   amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y, gRNA-ADE2.Y
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAR010 Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD

    Plasmid

    #195543

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-STAT3

    Plasmid

    #195575

    Purpose

    Expresses STAT3

    Depositor

    Kevin Park

    Article

    Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi

    Insert

    Signal transducer and activator of transcription 3 (Stat3 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV enhancer and promoter

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer

    Plasmid

    #176589

    Purpose

    Endogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacer

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    CRISPR-associate IscB (Chesapeake Bay) locus
    

    Expression

    Bacterial

    Mutation

    Truncated CRISPR array to two direct repeats, rep…

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pROS16

    Plasmid

    #107930

    Purpose

    HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Flt3-Y591F/Y919F

    Plasmid

    #74497

    Purpose

    Overexpression of FLT3 Y591F/Y919F mutant in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

    Insert

    FLT3 (FLT3 Human)
    

    Expression

    Mammalian

    Mutation

    Y591F/Y919F, T227M

    Promoter

    CMV

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pPB-cT3G-cERP2-ELK1

    Plasmid

    #192918

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of ELK1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    ELK1 (ELK1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTwist-SARS-CoV-2 Δ18 K417N+E484K+N501Y

    Plasmid

    #169469

    Purpose

    Encodes SARS-CoV-2 K417N+E484K+N501Y Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 K417N+E484K+N501Y Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. KCC2-pHext

    Plasmid

    #104076

    Purpose

    To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

    Depositor

    Igor Medyna

    Article

    Friedel et al eNeuro. 2017 Jul 21;4(4). pii: ENEURO.00

    Insert

    KCC2 (b isoform) (Slc12a5 Rat)
    

    Tags

    pHluorine tag inserted in the second putative tra…

    Expression

    Mammalian

    Mutation

    EcoR1 site in rat KCC2 was removed by silent muta…

    Promoter

    UbC

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. phBG-P53DD

    Plasmid

    #149707

    Purpose

    In vitro transcription of dominant negative form of P53 mRNA for RNA transfection into mammalian cells

    Depositor

    Erika Sasaki

    Article

    Watanabe et al Genes Cells. 2019 Jul;24(7):473-484. doi

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    Rna transcription

    Mutation

    deleted amino acid 14-301

    Promoter

    T7

    Available Since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV-Flt3-Y591F/Y919F

    Plasmid

    #74498

    Purpose

    Retroviral expression of FLT3 Y591F/Y919F

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Y591F/Y919F, T227M

    Promoter

    unknown

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pROS12

    Plasmid

    #107926

    Purpose

    hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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