We narrowed to 11,977 results for: pcDNA

1. pcDNA3.1+CMV-Ins-GLuc

   Plasmid

   #89929

   Purpose

   Expresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.

   Depositor

   Melanie Cobb

   Article

   Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E

   Insert

   human insulin-Gaussia-Luciferase (INS Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   DNA sequence for Gaussia luciferase was humanized…

   Promoter

   CMV

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1+_FH-AGO2-5xE

   Plasmid

   #92008

   Purpose

   Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   S824E, S828E, T830E, S831E, S834E (5xE)

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA5/FRT-ChemoL-CaM

   Plasmid

   #193830

   Purpose

   CMV overexpression of ChemoL-CaM in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoL-CaM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP:A206K, E299D; HT7: L271E

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA4-TO-Puromycin-mVenus-MAP

   Plasmid

   #44118

   Depositor

   Dannel McCollum

   Article

   Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

   Type

   Empty backbone

   Use

   Tags

   mVenus-MAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Tat101(AD8)-Flag

   Plasmid

   #115777

   Purpose

   pCMV-Tat101AD8-Flag allows the expression of Tat101(AD8) protein with a C-terminal flag-tag.

   Depositor

   Damian Purcell

   Article

   Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

   Insert

   HIV-1 Tat protein (AD8)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.3−3xFLAG−AGO2(D669A)

   Plasmid

   #220461

   Purpose

   Express catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Human AGO2 (AGO2 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Aspartate 669 to alanine

   Promoter

   CMV

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. human gamma-catenin pcDNA3

   Plasmid

   #16827

   Depositor

   Eric Fearon

   Article

   Caca et al Cell Growth Differ. 1999 Jun . 10(6):369

   Insert

   gamma-catenin (JUP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. mScarletI-T2A-mTq2 pcDNA3.1

   Plasmid

   #230048

   Purpose

   Expresses mScarletI-T2A-mTq2 in human cells

   Depositor

   Bas Teusink

   Article

   Botman et al bioRxiv 2024.11.29.626064

   Insert

   mScarletI-T2A-mTq2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNAintron-CHIKV-Envelope protein

   Plasmid

   #215700

   Purpose

   Produces the Chikungunya virus envelope protein E3-E2-6K-E1

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV E3-E2-6K-E1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNAintron-CHIKV-capsid protein

    Plasmid

    #215701

    Purpose

    Produces the Chikungunya virus capsid protein

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV capsid
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCDNA3-Flag-SPRTN-E112A

    Plasmid

    #110215

    Purpose

    Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.

    Depositor

    Kristijan Ramadan, Ivana Marinovic-Terzic

    Article

    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    E112A, P296L (see depositor comments below)

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT miniAID-EGFP

    Plasmid

    #101713

    Purpose

    For amplification of a miniAID-EGFP for C-terminal tagging

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    miniAID-EGFP
    

    Use

    Tags

    miniAID-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-HA-CUL1 delta N53

    Plasmid

    #19950

    Depositor

    Yue Xiong

    Article

    Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

    Insert

    cullin 1 delta N53 (CUL1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deletion of 53 residues from the NH2-terminus(de…

    Promoter

    Available since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-HA-CUL1 delta C22

    Plasmid

    #19942

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

    Insert

    cullin 1 delta C22 (CUL1 Human)
    

    Use

    Tags

    HA2

    Expression

    Mammalian

    Mutation

    Mutation of 22 amino acids from the C terminus

    Promoter

    Available since

    April 17, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1-TAT-1-101-FLAG

    Plasmid

    #35981

    Depositor

    Susana Valente

    Article

    Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

    Insert

    transactivating protein (tat )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    tat from HIV1 101 amino acids

    Promoter

    Available since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 deltaN47 Beta-catenin

    Plasmid

    #19287

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N-terminal truncation, deleted aa 1-47

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. Yellow Cameleon-Nano65/pcDNA3

    Plasmid

    #51965

    Purpose

    mammalian expression of Yellow Cameleon-Nano65, an ultrasensitive Ca2+ indicator with Kd = 65 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano65
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1 Hygro EYFP H148Q

    Plasmid

    #25873

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with H148Q mutation

    Promoter

    Available since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3. Flag-CSL R192H

    Plasmid

    #167005

    Purpose

    Mammalian expression of CSL R192H

    Depositor

    Gian-Paolo Dotto

    Article

    Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

    Insert

    CSL (RBPJ Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R192H

    Promoter

    CMV

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1/opto-b2AR-EYFP

    Plasmid

    #20948

    Purpose

    Mammalian expression of opto-b2AR fused to EYFP for optical control of GPCR signaling

    Depositor

    Karl Deisseroth

    Article

    Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

    Insert

    opto-b2AR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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