We narrowed to 17,384 results for: Ret;

1. pMSCV-FKBP-TRIF(Nterm)

   Plasmid

   #221244

   Purpose

   Expression of TRIF N-terminus (1-222) in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   TRIF N-terminus (1-222) (Ticam1 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG-4xFKBP

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Sema3e(L)-AP-His

   Plasmid

   #72019

   Purpose

   Expresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3e (Sema3e Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MP28445 – pFUSE2ss-aRD114_CLIg-mIgK

   Plasmid

   #110556

   Purpose

   pFUSE backbone with an IL2 signal sequence, expressing the variable light chain of anti-RD114 binder fused to murine Kappa constant light chain.

   Depositor

   Martin Pule

   Article

   Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

   Insert

   anit-RD114 light chain
   

   Use

   Tags

   mKappa

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF-1 alpha core promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEIAV-DIO-TLoop-CHR2YFP

   Plasmid

   #66798

   Purpose

   expression of channelrhodopsin-2-YFP, a light-gated cation channel that can depolarize cells via sodium influx when blue light changes its conformation, using a DIO-based TLoop EIAV vector

   Depositor

   Edward Callaway

   Article

   Cetin et al J Neurophysiol. 2014 May;111(10):2150-9.

   Insert

   Floxed Chr2-YFP
   

   Use

   Lentiviral

   Tags

   citrine

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CMVTetO7 Promoter

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. NPY-mApple

   Plasmid

   #83498

   Purpose

   Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

   Depositor

   Sebastian Barg

   Article

   Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

   Insert

   NPY (NPY Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBEG R2-iHygro-L3 #BGV206

   Plasmid

   #48985

   Purpose

   Supplies the selection marker (hygromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   hygromycin phosphotransferase
   

   Use

   Gateway cloning vector

   Tags

   IRES (weak)

   Expression

   Mutation

   Promoter

   na

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

7. CMV_Npu-ABEmax C-terminal

   Plasmid

   #137174

   Purpose

   Expresses the C-terminal split-intein fragment of ABEmax from the CMV promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   Npu-ABEmax_C-terminal
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CMV_Npu-ABEmax N-terminal

   Plasmid

   #137173

   Purpose

   Expresses the N-terminal split-intein fragment of ABEmax from the CMV promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   Npu-ABEmax_N-terminal
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 D10A

   Promoter

   CMV

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT7-EGFP-C1-HsNXF-1-5xmut_O

   Plasmid

   #147116

   Purpose

   Mammalian Expression of HsNxf-1-5xmut

   Depositor

   Elisa Izaurralde

   Article

   Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

   Insert

   HsNxf-1-5xmut (NXF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments Below

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOCC304

    Plasmid

    #118897

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with TEVprotease and TwinStrep-MB…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV.CMV.VTN.C-HA

    Plasmid

    #154849

    Purpose

    Expresses murine Vitronectin (VTN) protein with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Vitronectin (Vtn Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-2)

    Plasmid

    #159660

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-3)

    Plasmid

    #159661

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-1)

    Plasmid

    #159659

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Plxna4-AP-His

    Plasmid

    #71999

    Purpose

    Expresses the extracellular region of the PlexinA4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxna4 (Plxna4 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMXs-GLIS1 (FLJ33315SAAN)

    Plasmid

    #33274

    Purpose

    Retroviral expression of canonical human GLIS1

    Depositor

    Shinya Yamanaka

    Article

    Maekawa et al Nature. 2011 . 474(7350):225-9.

    Insert

    GLIS1, Gateway casette A (GLIS1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3 Flag MKK7a1 (glu)

    Plasmid

    #14540

    Depositor

    Roger Davis

    Article

    Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

    Insert

    MKK7a1(Glu) (Map2k7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser-198Glu Thr-202Glu

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pLPC-NMYC-mTRF1

    Plasmid

    #64162

    Purpose

    Retroviral vector expressing mouse TRF1 with N-terminal Myc tag

    Depositor

    Titia de Lange

    Article

    Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

    Insert

    mTRF1 (Terf1 Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pKG139

    Plasmid

    #69755

    Purpose

    Retroviral expression of CENP-C

    Depositor

    Iain Cheeseman

    Article

    Gascoigne et al Cell. 2011 Apr 29;145(3):410-22. doi: 10

    Insert

    CENP-C (CENPC Human)
    

    Use

    Retroviral

    Tags

    mCherry-TEV-S

    Expression

    Mammalian

    Mutation

    L341F, N570D relative to NP_001803.2 (numbering b…

    Promoter

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSCL.175

    Plasmid

    #184996

    Purpose

    Express -Eco1 HEK3 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    HEK3 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEasyG1_hph

    Plasmid

    #184912

    Purpose

    Template to generate via PCR a single gRNA for expression in S. cerevisiae

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET15b-sfGO-Matryoshka-LE-LP

    Plasmid

    #100018

    Purpose

    Bacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    cpsfGFP-LSSmOrange
    

    Use

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Leu and Glu N-term linker and Leu and Pro C-term …

    Promoter

    T7

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Beta1-GFP pFB-Neo

    Plasmid

    #69767

    Purpose

    Expresses Beta1-GFP integrin for retroviral transfection

    Depositor

    Martin Humphries

    Article

    Parsons et al J Cell Sci. 2008 Feb 1;121(Pt 3):265-71.

    Insert

    Beta1 integrin (ITGB1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    IRES

    Available since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

24. Str-STIM1-NN_ST-SBP-mCherry

    Plasmid

    #65263

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-SARS-CoV-2 Nsp3TM

    Plasmid

    #204206

    Purpose

    Mammalian expression of SARS-CoV-2 Nsp3

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
    

    Use

    Tags

    FLAGx1

    Expression

    Mammalian

    Mutation

    none, from obtained clone

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSCL.177

    Plasmid

    #184998

    Purpose

    Express -Eco1 EMX1 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    EMX1 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGL4.10-VEGFprom(-500 to -1)

    Plasmid

    #66130

    Purpose

    Luciferase-based reporter for VEGF promoter region (-500 to -1 bp)

    Depositor

    David Mu

    Article

    Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

    Insert

    Vascular Endothelial Growth Factor-A promoter region (VEGFA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    VEGF -500 to -1

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMSCV-IRES-hCD2-CD14

    Plasmid

    #133061

    Purpose

    Retroviral expression of mouse CD14

    Depositor

    Jonathan Kagan

    Article

    Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

    Insert

    CD14 (Cd14 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FUW-ubiquitin-SV40-GFP

    Plasmid

    #65445

    Purpose

    Expression of GFP in mammalian cells. Control vector for FUW-ubiquitin-EphB-SV40-GFP vectors

    Depositor

    Eduard Batlle

    Article

    Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

    Insert

    SV40-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBQ and SV40

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pEF1a-2xSV40_NLS-NLuc

    Plasmid

    #135953

    Purpose

    Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    NanoLuciferase
    

    Use

    Tags

    2xSV40-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge