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Showing: 8481 - 8520 of 8661 results
  1. pCDNA3.1-nAChr-beta3-YFP

    Plasmid
    #50484
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    YFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
  2. pCAG-tdTHC

    Plasmid
    #112621
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2
    Depositor
    Inserts
    H2B-tdTomato (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Cre/Lox
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pCAG-VHC

    Plasmid
    #112618
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2
    Depositor
    Inserts
    H2B-Venus (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pAAV-hSyn-DIO {mCAR}off-{ChETA-mRuby2}on-W3SL

    Plasmid
    #111390
    Purpose
    AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)
    Depositor
    Inserts
    mCAR (Cxadr Mouse)
    ChETA-mRuby2
    Use
    AAV
    Tags
    Myc and mRuby2
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pLH_Scr16

    Plasmid
    #100538
    Purpose
    CEN/ARS plasmid with PhyBNT-NLS-CreN and PIF3-NLS-CreC expression cassettes for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.
    Inserts
    Phytochrome B (PHYB Mustard Weed)
    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Cdc1 NLS, CreC, CreN, and SV40 NLS
    Expression
    Yeast
    Mutation
    AA 1-621 of PhyB/ Mutation nucleotide 576 C-->A
    Promoter
    FBA1 and TDH3
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pAAV-hSyn-DIO {hCAR}off-{ChETA-mRuby2}on-W3SL

    Plasmid
    #111391
    Purpose
    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)
    Depositor
    Inserts
    hCAR (CXADR Human)
    ChETA-mRuby2
    Use
    AAV
    Tags
    Myc and mRuby2
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. pCAG-mTHC

    Plasmid
    #112620
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2
    Depositor
    Inserts
    H2B-mTFP1 (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Tags
    mTeal (mTFP1)
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pEN527 - Rad21-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

    Plasmid
    #156452
    Purpose
    Targeting vector to introduce an AID-eGFP cassette at the mouse RAD21 (SCC1) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA CCACGGTTCCATATTATCTG
    Depositor
    Insert
    RAD21, AID (Rad21 Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    none
    Available Since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. pLKO_S.AU1.VSVg_NGFR

    Plasmid
    #158351
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    S.AU1.VSVg
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pSin-EF2-DMD53-GFP

    Plasmid
    #138353
    Purpose
    Expressing a GFP reporter cassette, whose GFP reading frame is disrupted by insertion of partial DMD exon 53 sequences
    Depositor
    Insert
    DMD exon 53 partial sequence to disrupt GFP reading frame (DMD Human)
    Use
    CRISPR and Lentiviral
    Tags
    No
    Expression
    Mammalian
    Promoter
    EF2
    Available Since
    Nov. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. pAAV-hSyn-DIO {hCAR}off-{ChETA-EYFP}on-W3SL

    Plasmid
    #111392
    Purpose
    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-EYFP (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)
    Depositor
    Inserts
    hCAR (CXADR Human)
    ChETA-EYFP
    Use
    AAV
    Tags
    EYFP and Myc
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
  12. pLKO_V5.AU1.VSVg_NGFR

    Plasmid
    #158241
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    V5.AU1.VSVg
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. ptdTHC

    Plasmid
    #112617
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2
    Depositor
    Inserts
    H2B-tdTomato (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    No Promoter
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
  14. pLKO_HA.AU1.VSVg_NGFR

    Plasmid
    #158232
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.AU1.VSVg
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  15. pLKO_NWS.AU1.VSVg_NGFR

    Plasmid
    #158239
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    NWS.AU1.VSVg
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  16. pVHC

    Plasmid
    #112614
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2
    Depositor
    Inserts
    H2B-Venus (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    No Promoter
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
  17. pLKO_V5.AU1.FLAG_NGFR

    Plasmid
    #158240
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    V5.AU1.FLAG
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  18. pLKO_NWS.V5.VSVg_NGFR

    Plasmid
    #158246
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    NWS.V5.VSVg
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  19. pLKO_V5.FLAG.VSVg_NGFR

    Plasmid
    #158247
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    V5.FLAG.VSVg
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  20. pLKO_NWS.V5.FLAG_NGFR

    Plasmid
    #158251
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    NWS.V5.FLAG
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  21. pLKO_S.V5.HA_NGFR

    Plasmid
    #158339
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    S.V5.HA
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  22. pLKO_Ollas.AU1.C_NGFR

    Plasmid
    #158314
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    Ollas.AU1.C
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  23. Drd1a->M71-IRES-tauGFP ACNF TV

    Plasmid
    #105073
    Purpose
    Targeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-447 of Drd1a and an IRES-tauGFP followed by ACNF cassette
    Depositor
    Insert
    Drd1a->M71-IRES-tauGFP-ACNF (Drd1, Olfr151 Mouse)
    Use
    Mouse Targeting
    Mutation
    Drd1a coding sequence followed by IRES-tauGFP ACNF
    Available Since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  24. pLKO_HA.AU1.V5_NGFR

    Plasmid
    #158235
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.AU1.V5
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  25. pGG369

    Plasmid
    #165606
    Purpose
    Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)
    Depositor
    Insert
    ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…
    Expression
    Bacterial
    Mutation
    Recoding of BsaI site in the AmpR cassette, two B…
    Promoter
    lacUV2 driving ω-dCas9 and UV5 driving sgRNA
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
  26. pAJ1

    Plasmid
    #138379
    Purpose
    pEcRNAP6 derivative encoding Escherichia coli core RNA polymerase subunits: RpoA, RpoB, RpoC(His)10, RpoZ
    Depositor
    Inserts
    RpoA (rpoA E. coli)
    RpoB (rpoB E. coli)
    RpoC (rpoC E. coli)
    rpoZ (rpoZ E. coli)
    Tags
    His10
    Expression
    Bacterial
    Mutation
    Clamp toe deletion: Δ(Tyr144-Lys179) ::(G144-S145…
    Promoter
    T7
    Available Since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
  27. pCAG-EHC

    Plasmid
    #112619
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2
    Depositor
    Inserts
    H2B-Emerald (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  28. pEHC

    Plasmid
    #112615
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2
    Depositor
    Inserts
    H2B-Emerald (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    Emerald
    Expression
    Mammalian
    Promoter
    No Promoter
    Available Since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
  29. pmTHC

    Plasmid
    #112616
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2
    Depositor
    Inserts
    H2B-mTFP1 (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    mTeal (mTFP1)
    Expression
    Mammalian
    Promoter
    No Promoter
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
  30. pDONR P2R-P3-IRES_ECFP

    Plasmid
    #48354
    Purpose
    Contributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules.
    Insert
    IRES_ECFP
    Use
    Gateway entry vector
    Mutation
    ECFP translation is mediated by an IRES. Contains…
    Promoter
    none
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
  31. pCDNA3.1-nAChr-beta3-CFP

    Plasmid
    #50485
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    CFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 8481 - 8520 of 8661 results