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pBabe H-Ras 12V

Plasmid

#12545

Depositor

Channing Der

Publication

Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

Insert

H-Ras 12V (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

12V constituitively activated.

Available since

Sept. 8, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
LL - hOCT4i -2

Plasmid

#12197

Depositor

George Daley

Publication

Zaehres et al Stem Cells. 2005 Mar . 23(3):299-305.

Insert

OCT4 - RNAi (POU5F1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available since

Sept. 6, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHW394 (15xUAS::GFP::let-858 3'UTR)

Plasmid

#85584

Purpose

cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans

Depositor

Paul Sternberg

Publication

Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

15xUAS-delta pes-10-GFP

Expression

Worm

Mutation

GFP(S65C) with three introns for expression in C.…

Promoter

15xUAS-delta pes-10-GFP

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA-HA-ER Y537S

Plasmid

#49499

Purpose

Expresses HA-tagged ER Y537S mutant in mammalian cells

Depositor

Sarat Chandarlapaty

Publication

Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

Insert

ESR1 (ESR1 Human)

Tags

HA

Expression

Mammalian

Mutation

Tyrosine 537 to Serine

Promoter

CMV

Available since

Dec. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHM6-Q23

Plasmid

#40263

Depositor

David Rubinsztein

Publication

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HTT partial exon 1 Q23 (HTT Human)

Tags

6x His and HA

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pEGFP-MRLC1 T18D, S19D

Plasmid

#35682

Depositor

Tom Egelhoff

Publication

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MRLC1 (MYL9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T18D and S19D

Promoter

CMV

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles
pPaxillin-PSmOrange

Plasmid

#31923

Depositor

Vladislav Verkhusha

Publication

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PSmOrange

Tags

Paxillin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

Plasmid

#86827

Purpose

Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

Depositor

Carl Novina

Publication

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

FLAG-NLS-MS2-eGFP

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pET/ELP-I-CAT

Plasmid

#71461

Purpose

ELP-intein purification system

Depositor

David Wood

Publication

Banki et al Nat Methods. 2005 Sep;2(9):659-61.

Type

Empty backbone

Tags

ELP ((VPGXG)110) and intein

Expression

Bacterial

Available since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Flag-FOXA1

Plasmid

#153109

Purpose

Expression of Flag tagged human FOXA1 in mammalian cells

Depositor

Stefan Koch

Publication

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXA1 (FOXA1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-HA-RIPK3

Plasmid

#78804

Purpose

To overexpress RIPK3 in Mammalian Cells

Depositor

Jaewhan Song

Publication

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pSCAR_sgRNA_puro-mKate-lox2272

Plasmid

#162076

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

Depositor

Robert Manguso

Publication

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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hSyn1-sypHy-RGECO

Plasmid

#84078

Purpose

Combined fluorescent reporter for both presynaptic calcium influx and vesicle excoytosis

Depositor

Juan Burrone

Publication

Jackson et al Front Synaptic Neurosci. 2016 Jul 26;8:2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sypHy-RGECO

Expression

Mammalian

Promoter

hSyn1

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-ALK5 T204D

Plasmid

#80877

Purpose

mammalian expression of constitutively active ALK5 T204D

Depositor

Aristidis Moustakas

Publication

Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ALK5 (TGFBR1 Human)

Tags

HA

Expression

Mammalian

Mutation

T204D (constitutively active)

Promoter

CMV

Available since

Aug. 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pRK-flag-USP19

Plasmid

#78597

Purpose

Express flag-USP19 in mammalian cells

Depositor

Yihong Ye

Publication

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

flag-USP19 (USP19 Human)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1 3xFlag IFIT1

Plasmid

#53554

Purpose

mammalian expression of IFIT1

Depositor

Kathleen Collins

Publication

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT1 (IFIT1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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GFP-ERK2

Plasmid

#37145

Depositor

Rony Seger

Publication

Rubinfeld et al J Biol Chem. 1999 Oct 22;274(43):30349-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ERK2 (MAPK1 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

aa1-6 deleted as a result of cloning strategy

Promoter

CMV

Available since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pJMK004

Plasmid

#34616

Purpose

Viral expression of Arch (D95N mutant with greater sensitivity but slower response) voltage indicator

Depositor

Adam Cohen

Publication

Kralj et al Nat Methods. 2011 Nov 27. doi: 10.1038/n

Insert

Arch D95N

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Changed Aspartic Acid 95 to Asparagine

Promoter

CaMKII

Available since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLNCX2-Scrib

Plasmid

#24600

Depositor

Senthil Muthuswamy

Publication

Zhan et al Cell. 2008 Nov 28. 135(5):865-78.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Scribble (SCRIB Human)

Expression

Mammalian

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p3xnls-mScarlet3_N1

Plasmid

#189775

Purpose

Construct for labelling cell nuclei in mammalian cells using nuclear targeted mScarlet3

Depositor

Dorus Gadella

Publication

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

3xnls-mScarlet3

Expression

Mammalian

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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