We narrowed to 5,494 results for: SPI

1. pST_DrHD_dom3_k5_0 (pBS0758)

   Plasmid

   #185242

   Purpose

   For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k5_0. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   DrHD_dom3_k5_0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pST_DrHD_dom3_k3_7 (pBS0755)

   Plasmid

   #185241

   Purpose

   For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k3_7. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   DrHD_dom3_k3_7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pST_DrHD_dom3_k3_1 (pBS0754)

   Plasmid

   #185240

   Purpose

   For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k3_1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   DrHD_dom3_k3_1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pST_CI016_HUMAN (pBS0747)

   Plasmid

   #185238

   Purpose

   For the mammalian expression of the human protein CI016_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   CI016_HUMAN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pST_AdAPOA4-44 (pBS0745)

   Plasmid

   #185236

   Purpose

   For the mammalian expression of the Chinese giant salamander protein AdAPOA4-44. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   AdAPOA4-44
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMSCV-IRES-GFP-Hybrid-caspase_mouse-caspase-11-CARD+canine-Caspase-1/4-catalytic domain

   Plasmid

   #183398

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   CARD domain of mouse caspase-11 fused to catalytic domain of canine caspase-1/4
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMSCV-IRES-GFP-human-Caspase-1-H340D

   Plasmid

   #183360

   Purpose

   Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1 H340D (CASP1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   H340D

   Promoter

   MESV

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMSCV-IRES-GFP-human-caspase-1/4b

   Plasmid

   #183394

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   CARD domain of human caspase-1 fused to human caspase-4 (CASP1 Synthetic, Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDK616

   Plasmid

   #134202

   Purpose

   Bacterial expression of human Ska1 microtubule binding domain (MTBD) mutant

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-Precission-human Ska1 MTBD (SKA1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   K223A K226A

   Promoter

   Available since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIW040

    Plasmid

    #134127

    Purpose

    Bacterial expression of human Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-Precission-human Ska1 MTBD (SKA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    aa 132-255 R236A

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIW039

    Plasmid

    #134126

    Purpose

    Bacterial expression of human Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-Precission-human Ska1 MTBD (SKA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    aa 132-255 and K203A K206A

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIW028

    Plasmid

    #134125

    Purpose

    Bacterial expression of human Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-Precission-human Ska1 MTBD (SKA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    aa 132-255 and K183A K184A

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIW070

    Plasmid

    #134138

    Purpose

    Retroviral expression of GFP-Ska1

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    GFP-human Ska1 delta MTBD CRISPR/Cas9 hardened (SKA1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    residues 1-131, CRISPR resistant

    Promoter

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIGc-Xph18

    Plasmid

    #133029

    Purpose

    Expresses Xph18 in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph18
    

    Use

    Tags

    His10

    Expression

    Bacterial

    Mutation

    S63K

    Promoter

    T7

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIGc-Xph20

    Plasmid

    #133024

    Purpose

    Expresses Xph20 in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph20
    

    Use

    Tags

    His10

    Expression

    Bacterial

    Mutation

    S63K

    Promoter

    T7

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIGc-Xph15

    Plasmid

    #133030

    Purpose

    Expresses Xph15 in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph15
    

    Use

    Tags

    His10

    Expression

    Bacterial

    Mutation

    S63K

    Promoter

    T7

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5-LAP-BUB1

    Plasmid

    #115903

    Purpose

    Used for expression of siRNA-resistant BUB1-WT from the FRT site of FlpIn cells upon addition of doxycyclin.

    Depositor

    Geert Kops

    Article

    Vleugel et al J Cell Sci. 2015 Aug 15;128(16):2975-82.

    Insert

    BUB1 (BUB1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5-hygro-eGFP-STOP-HEC1

    Plasmid

    #114026

    Purpose

    Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-STOP-HEC1 (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Start codon of Hec1 is mutated to stop codon

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBac-LacI-LAP-HEC1

    Plasmid

    #114033

    Purpose

    Production of baculovirus with LacI-LAP-HEC1

    Depositor

    Geert Kops

    Article

    Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

    Insert

    LacI-LAP-HEC1 (NDC80 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-shScn4b-268

    Plasmid

    #99031

    Purpose

    Expresses a shRNA targeting the mouse Scn4b gene

    Depositor

    Amy Lasek

    Article

    Ji et al Front Cell Neurosci. 2017 Feb 13;11:17.

    Insert

    Scn4b shRNA (Scn4b Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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