We narrowed to 22,191 results for: his

1. wt HIV-1 integrase in pET15b

   Plasmid

   #61668

   Purpose

   wild type HIV-1 integrase in pET-15b

   Depositor

   Robert Craigie

   Article

   Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

   Insert

   wild type HIV-1 integrase
   

   Use

   Tags

   His

   Expression

   Mutation

   Promoter

   T7 promotor

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

2. enz.083-NMT1

   Plasmid

   #154047

   Purpose

   Expresses NMT1 in E. coli

   Depositor

   Christopher Voigt

   Article

   Wallace et al Advanced Functional Materials 2020

   Insert

   NMT1 (N-myristoyltransferase 1) (NMT1 Human)
   

   Use

   Tags

   His, MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pmAmetrine1.2

   Plasmid

   #42171

   Depositor

   Robert Campbell

   Article

   Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

   Insert

   mAmetrine1.2
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available Since

   Feb. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. YeNL_pRSETb

   Plasmid

   #89520

   Purpose

   Yellow color variant of bright luminescent protein for bacterial expression

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   YeNL
   

   Use

   Tags

   His X 6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1(+)-deltaFlincG

   Plasmid

   #19734

   Depositor

   Wolfgang Dostmann

   Article

   Nausch et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

   Insert

   delta-FlincG
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   aa 77-356

   Promoter

   Available Since

   March 26, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pET28_mouse-Caspase-11_enzymatic-domain

   Plasmid

   #183382

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-11 (AA100-373) (Casp4 Mouse)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-gSTEP1

   Plasmid

   #159376

   Purpose

   Expresses gSTEP1 in E. coli

   Depositor

   Roberto Chica

   Article

   Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

   Insert

   gSTEP1
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDEST26-hOPTN

   Plasmid

   #23050

   Depositor

   Jon Ashwell

   Article

   Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43.

   Insert

   Optineurin (OPTN Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available Since

   Jan. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pNR64

   Plasmid

   #79540

   Purpose

   Input plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.

   Depositor

   Timothy Lu

   Article

   Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

   Inserts

   TP901
   BxbI
   

   Use

   Tags

   His tag and LAA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCRISPRed pRSETB

    Plasmid

    #159454

    Purpose

    Expresses the pH-resistant long Stokes shift red fluorescent protein mCRISPRed

    Depositor

    Oliver Griesbeck

    Article

    Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-62

    Insert

    mCRISPRed
    

    Use

    Tags

    10 x His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGWB701NL3F10H

    Plasmid

    #141288

    Purpose

    No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, Tunicamycin R for plant)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Type

    Empty backbone

    Use

    Luciferase; Gateway

    Tags

    FLAG (x3), HIS (x10), and NLUC

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYES2.1-pomMet8

    Plasmid

    #107449

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    Met8
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET30b-7Y-SrtA

    Plasmid

    #105601

    Purpose

    expresses 7Y sortase A variant in E.coli

    Depositor

    Stephanie Dougan

    Article

    Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

    Insert

    S.aureus Sortase A, 7Y mutant
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-Neq2X

    Plasmid

    #182365

    Purpose

    Expresses Neq2X DNA polymerase

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

    Insert

    Neq2X
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAD6

    Plasmid

    #134349

    Purpose

    E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

    Depositor

    Seth Darst

    Article

    Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

    Insert

    mfd (mfd E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIA1414

    Plasmid

    #172402

    Purpose

    Expression of SARS-COV-2 Nsp9 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp9 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCellFree_G03 PC

    Plasmid

    #67132

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PC (PC Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pYES2.1-cerMET8

    Plasmid

    #107439

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET8 (MET8 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. OeNL_pRSETb

    Plasmid

    #89529

    Purpose

    Orange color variant of bright luminescent protein for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    OeNL
    

    Use

    Tags

    His X 6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS-PSD95-GCaMP2

    Plasmid

    #18931

    Depositor

    Karel Svoboda

    Article

    Mao et al PLoS ONE. 2008 . 3(3):e1796.

    Insert

    PSD95-GCaMP2 (Dlg4 Rat)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

21. pfMDH_WT

    Plasmid

    #67721

    Purpose

    Expresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pCMV-P-RAN-GFP26

    Plasmid

    #106406

    Purpose

    P-RAN-GFP26 (RANbody GFP26::vHRP)

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

    Insert

    P-RAN-GFP26
    

    Use

    Tags

    HA and His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET24b-BR1.0

    Plasmid

    #199435

    Purpose

    expresses BAmRed1.0 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMRed
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pIA1400

    Plasmid

    #172519

    Purpose

    Expression of SARS-COV-2 Nsp12 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-51b-HaloTag10

    Plasmid

    #175520

    Purpose

    Expression of HaloTag10 in bacterial cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag10
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    HaloTag10 = HaloTag7-Q165W

    Promoter

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-Y-RAN-GFP26

    Plasmid

    #106423

    Purpose

    Y-RAN-GFP26 (RANbody GFP26::Chicken IgY)

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

    Insert

    Y-RAN-GFP26
    

    Use

    Tags

    HA and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ZAK (ZAKA-c057)

    Plasmid

    #98225

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ZAKA (MAP3K20 Human)
    

    Use

    Baculovirus

    Tags

    His-6-TEV

    Expression

    Mutation

    pfam00069:Pkinase 16-257

    Promoter

    polyhedrin

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCellFree_G03 CYP2A13

    Plasmid

    #67103

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CYP2A13 (CYP2A13 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pGBHPS-SortA

    Plasmid

    #21930

    Depositor

    Fuyuhiko Inagaki

    Article

    Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

    Insert

    sortase
    

    Use

    Tags

    GB1, HRV3C cleagage site, and His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    Available Since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBbA5k-EPL14

    Plasmid

    #45405

    Depositor

    Aindrila Mukhopadhyay

    Article

    Dunlop et al Mol Syst Biol. 2011 May 10;7:487. doi: 1

    Insert

    mexF, PP_3426
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Mutation

    Promoter

    LacUV5

    Available Since

    Jan. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRset-OppA

    Plasmid

    #207869

    Purpose

    Bacterial expression of poly-his-tagged L. lactic OppA

    Depositor

    Mathew Tantama

    Article

    Norcross et al ACS Omega. 2019 Feb 28;4(2):2812-2822. d

    Insert

    OppA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGWB601NL3F10H

    Plasmid

    #141287

    Purpose

    No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, BASTA R for plant)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Type

    Empty backbone

    Use

    Luciferase; Gateway

    Tags

    FLAG (x3), HIS (x10), and NLUC

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMCSG7-dNmeCas9

    Plasmid

    #71475

    Purpose

    bacterial pMCSG7 expression vector expressing catalytically inactive Nme cas9 with T7 promoter, N-terminal His tag and TEV site

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

    Insert

    pMCSG7-Inactive-NmeCas9
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    D16A H588A

    Promoter

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. GFP S2TAG

    Plasmid

    #68296

    Purpose

    S2TAG GFP reporter

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    GFP S2TAG
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. TAF1

    Plasmid

    #39118

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TAF1 (TAF1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

36. pYES2.1-pomMet26

    Plasmid

    #107448

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    Met26 (met26 Fission Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pYES2.1-pomMet16

    Plasmid

    #107452

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    Met16 (met16 Fission Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pYOY178

    Plasmid

    #23108

    Depositor

    Linda Wordeman

    Article

    Andrews et al Dev Cell. 2004 Feb . 6(2):253-68.

    Insert

    Mitotic Centromere Associated Kinesin
    

    Use

    Tags

    GFP and His

    Expression

    Mammalian

    Mutation

    S92A, S106A, S108A, S112A, S186A

    Promoter

    Available Since

    Feb. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. JARID1BA

    Plasmid

    #74646

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JARID1BA (KDM5B Human)
    

    Use

    Baculovirus

    Tags

    His-6-TEV

    Expression

    Mutation

    Leu101-(Gly)4-Ala374 deletion mutant

    Promoter

    PH

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

40. pIA1364

    Plasmid

    #172401

    Purpose

    Expression of SARS-COV-2 Nsp9 (extra GS at the N-terminal) in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp9 (ORF1ab Synthetic)
    

    Use

    Tags

    His-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge